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Co-inheritance of alpha globin gene deletion lowering serum iron level in female beta thalassemia patients

  • Sayed AbdulAzeez
  • Noor B. Almandil
  • Zaki A. Naserullah
  • Sana Al-Jarrash
  • Ahmed M. Al-Suliman
  • Huda I. ElFakharay
  • J. Francis BorgioEmail author
Original Article
  • 36 Downloads

Abstract

In the Eastern province of Saudi Arabia, thalassemia is highly common. Data on the effect of alpha globin gene variation on the concentration of iron on transfusion dependent Saudis are scanty. A total of 166 transfusions dependent β-thalassemia were included in this study to understand association between the alpha globin gene variation and concentration of iron. Using multiplex PCR, the alpha globin gene deletions were identified. Also, HBA1 and HBA2 genes were sequenced by Sanger sequencing. Saudi transfusion dependent female β-thalassemia patients with wild alpha globin genotype (αα/αα) were observed with iron level beyond the normal range. However, normal range of iron was observed in transfusion dependent Saudi female beta thalassemia patients co-inherited with double (-α3.7/-α3.7, or --Fil/αα or --MED/αα or - (α) 20.5/αα) or double heterozygosity (- -/-α3.7) alpha globin gene deletions, which is significantly (p < 0.0001) less compared to the Saudi transfused female with wild alpha globin genotype (αα/αα). The co-inheritance alpha globin gene deletions in female beta thalassemia patients were significantly lowering serum iron. Detailed studies can be taken forward to identify the molecular pathways involved in globin gene deletion as modulator.

Keywords

3.7 deletion Alpha globin gene Thalassemia Saudi Arabia, iron load 

Notes

Acknowledgements

The authors thank The Dean, Institute for Research and Medical Consultations (IRMC), for her continuous support and encouragement and the administrative staff and facilities provided at IRMC, especially the technical assistance from Mr. Ranilo M. Tumbaga, Mr. Horace T. Pacifico, and Ms. Jee E. Aquino. This study was supported by The Deanship of Scientific Research, Imam Abdulrahman Bin Faisal University (To JFB: 2012186 and 2014024; To SAA 2016-077-IRMC; 2014051), King Abdulaziz City for Science and Technology (To JFB LGP-35-204; and LGP-32-3) and National Science Technology and Innovation Plan (To JFB, Grant No: 12-MED 2798-46).

Compliance with ethical standards

Conflicts of interest

The authors declare that they do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Genetic Research, Institute for Research and Medical Consultation (IRMC)Imam Abdulrahman Bin Faisal UniversityDammamSaudi Arabia
  2. 2.Department of Clinical Pharmacy Research, Institute for Research and Medical Consultation (IRMC)Imam Abdulrahman Bin Faisal UniversityDammamSaudi Arabia
  3. 3.Dammam Maternity and Child HospitalDammamSaudi Arabia
  4. 4.King Faisal UniversityAl-AhssaSaudi Arabia
  5. 5.Almana General HospitalAl-KhobarSaudi Arabia

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