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Isoquercetin regulates SREBP-1C via AMPK pathway in skeletal muscle to exert antihyperlipidemic and anti-inflammatory effects in STZ induced diabetic rats

  • Muthukumaran Jayachandran
  • Tongze Zhang
  • Ziyuan Wu
  • Yinhua Liu
  • Baojun XuEmail author
Original Article
  • 20 Downloads

Abstract

Diabetes mellitus (DM) is a cluster of metabolic diseases that exhibits high blood glucose levels accompanied by hyperlipidemia and inflammation. DM is the primary risk factor contributes majorly to cardiovascular disease (CVD) mediated morbidity and mortality. The incidence of dyslipidemia seems to attribute considerably to the initiation of CVDs. The beneficial action of isoquercetin on hyperlipidemia and related signaling pathways are not documented yet, hence we decide to carry out this study. The experimental rats were divided into five groups: Group 1, control rats; group 2, isoquercetin control (40 mg/kg b.w); group 3, diabetic rats (STZ-40 mg/kg b.w); group 4, diabetic + isoquercetin (40 mg/kg b.w); and group 5, diabetic + glibenclamide (600 µg/kg b.w). The animals were sacrificed at the end of the experimental duration of 45 days. Results of our analysis reveal that isoquercetin have a major impact on the tissue lipid profile, isoquercetin strongly regulates the expression of various lipid-metabolizing enzymes, C-reactive protein, expression of various inflammatory genes, SREBP-1C genes and proteins and AMP-activated protein kinase-α (AMPK) signaling pathway genes and proteins. Results recommend that isoquercetin can be effective in mitigating the consequences of hyperlipidemia and DM.

Graphic Abstract

Keywords

Streptozotocin Hyperlipidemia HMG-Co-A AMPK-α SREBP-1C 

Abbreviations

ACC

Acetyl-CoA carboxylase

AMPK-α

AMP-activated protein kinase-α

DM

Diabetes mellitus

FAS

Fatty acid synthase

HDL

High-density lipoprotein

HMG Co-A

3-Hydroxy 3-methyl glutaryl coenzyme A

LCAT

Lecithin cholesterol acyltransferase

LDL

Low-density lipoprotein

LPL

Lipoprotein lipase

PL

Phospholipids

STZ

Streptozotocin

SREBP-1C

Sterol regulatory element-binding protein-1

TC

Total cholesterol

T2DM

Type 2 diabetes mellitus

TG

Triglycerides

Notes

Acknowledgements

Authors thank Ms. Yinhua Liu in Zhuhai Campus of Zunyi Medical University for her technical assistance on the use of the animal facility.

Author contributions

BX and SSMC designed, written and proofread the manuscript, MJ designed, carried out the experiments, written and proofread the manuscript, TZ helped in feeding the animals and dissection, ZW helped in carrying out few molecular studies.

Funding

The work was jointly supported by two grants R201714 and R201914 from Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, Guangdong, China.

Compliance with ethical standards

Conflict of interest

Authors declare no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Food Science and Technology ProgramBeijing Normal University-Hong Kong Baptist University United International CollegeZhuhaiChina
  2. 2.Biomedicine Research and Development CenterZhuhai Campus of the Zunyi Medical UniversityZhuhaiChina

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