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Cytotoxic, antimicrobial and nitric oxide inhibitory activities of supercritical carbon dioxide extracted Prunus persica leaves

  • Halil KoyuEmail author
  • Aslihan Kazan
  • Ayse Nalbantsoy
  • Husniye Tansel Yalcin
  • Ozlem Yesil-Celiktas
Original Article
  • 44 Downloads

Abstract

Different parts of Prunus persica as fruits, flowers, leaves and kernels have been consumed with dietary and therapeutic purposes traditionally. During fruit production, remarkable amount of leaves which can hold important bioactive groups as phenolics, have been left unutilized. The aim of this study was to investigate cytotoxic, antimicrobial and nitric oxide inhibitory activities of supercritical carbondioxide extracts of Prunus persica leaves. Among studied cell lines, supercritical carbon dioxide extract which was processed at 150 bar, 60 °C, and 6% co-solvent ethanol, exhibited remarkable cytotoxic activity against HeLa, MPanc-96 and MCF-7 cell lines with IC50 values of 12.22 µg/ml, 28.17 µg/ml and 35.51 µg/ml respectively, whereas IC50 value of conventional solvent extract was above 50 µg/ml. Minimum inhibitory concentration values determined for antibacterial and antifungal activities against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium and Candida albicans were found as 62.50 µg/ml. Strong nitric oxide inhibition was achieved with IC50 of 9.30 µg/ml. The promising results revealed that Prunus persica leaves may have remarkable potential as supplement both for drug and food industries. This study is the first report revealing cytotoxic, antimicrobial and nitric oxide inhibitory activity of supercritical carbon dioxide extract of Prunus persica leaves.

Keywords

Prunus persica Peach leaves Supercritical fluid extraction Cytotoxic Antimicrobial Nitric oxide inhibitory 

Notes

Acknowledgements

Access to the facilities of Novel Fluidic Technologies Laboratory at Department of Bioengineering, Pharmaceutical Sciences Research Center (FABAL) and IKCU EFAL at Faculty of Pharmacy are highly appreciated.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11033_2019_5163_MOESM1_ESM.docx (763 kb)
Supplementary material 1 (DOCX 763 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Pharmaceutical Botany, Faculty of PharmacyIzmir Katip Celebi UniversityIzmirTurkey
  2. 2.Department of Bioengineering, Faculty of EngineeringEge UniversityIzmirTurkey
  3. 3.Department of Biology, Faculty of ScienceEge UniversityIzmirTurkey
  4. 4.Biomedical Technologies Graduate Programme, Graduate School of Natural and Applied SciencesEge UniversityIzmirTurkey

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