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The protective effect of quercetin in the alcohol-induced liver and lymphoid tissue injuries in newborns

  • Erdal InceEmail author
Original Article
  • 69 Downloads

Abstract

Recently published experimental and clinical studies indicate that oxidative stress leads to the pathogenesis and progression of alcohol-induced tissue injuries. Quercetin is a type of flavonoid compound that influences antioxidant and anti-inflammatory activities have protective and therapeutic effects for treating various diseases including diabetes mellitus and neuro-degenerative diseases. In this study, fetal alcohol syndrome was tested in rat models, with the aim of verifying the protective effect of quercetin in preventing alcohol-induced liver and lymphoid tissue (thymus, spleen, and lymph nodes) injuries on the 21st day for the offspring of alcohol treated mother rats. The pregnant rats were randomly assigned into four groups. The control group (C) (n = 3) of pregnant rats received only physiological saline intraperitoneally (i.p.) throughout the pregnancy (1 to 21 days gestation) and during lactation until postnatal day 21. The quercetin positive control group (QT) of pregnant rats (n = 3) received quercetin at 50 mg/kg/days i.p. for the same period. The ethanol treatment group (E) (n = 3) of pregnant rats received 1 ml/day of 40% v/v ethanol (4 g/kg) intragastrically (i.g) for the same period. The model group of pregnant rats (EQ) received ethanol + quercetin (n = 3) with a dose of 1 ml/day of v/v ethanol (4 g/kg i.g.) and quercetin at 50 mg/kg body weight per day i.p. for the same period. Ten offspring were used in each of the C, QT, E and EQ groups. Malondialdehyde (MDA), protein carbonyl content (PC) and chemiluminescence levels (CL) in liver and lymphoid tissues significantly increased in group E versus the C group (P < 0.05–P < 0.001) whereas glutathione levels (GSH), glutathione reductase (GR), glutathione peroxidase (GP), superoxide dismutase (SOD), and catalase (CAT) activities significantly decreased in group E compared to the C group (P < 0.05–< 0.001). However, tissue MDA, PC, and CL levels decreased in the EQ group compared to group E. GSH level, GP, GR, SOD, and CAT activity were significantly increased by quercetin (P < 0.05–P < 0.001). The plasma TNFα, IL-1β, and IL-6 levels and NF-κB activation significantly increased in group E compared to the C and QT groups, but IL-10 significantly decreased in group E compared to the C and QT groups. The TNFα, IL-1β, and IL-6 levels and NF-κB activation significantly decreased in group EQ compared to group E. In conclusion, quercetin has a protective effect against maternal alcohol-induced oxidative and inflammatory damage in the liver and lymphoid tissues of newborn rats.

Keywords

Maternal alcohol Oxidative stress Quercetin Inflammatory responses Lymphoid tissues 

Abbreviations

FASD

Fetal alcohol spectrum disorder

ROS

Reactive oxygen species

LP

Lipid peroxidation

PC

Protein carbonyl content

CL

Chemiluminescence assay

GSH

Reduced glutathione

GP

Glutathione peroxidase

GR

Glutathione reductase

SOD

Superoxide dismutase

CAT

Catalase

MDA

Malondialdehyde

NF-κB

Nuclear factor kappa B

PIC

Proinflammatory cytokine

Notes

Compliance with ethical standards

Conflict of interest

The author has no financial or any other conflict of interest in this manuscript with third parties. The author is responsible for the content and writing of this paper.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Medical Science Biology, Cerrahpasa Medical FacultyIstanbul University—CerrahpasaFatih/IstanbulTurkey

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