Assessment of alpha glucosidase inhibitors produced from endophytic fungus Alternaria destruens as antimicrobial and antibiofilm agents
Diabetes is considered as a major health concern worldwide and patients with diabetes are at high risk for infectious diseases. Therefore, α-glucosidase inhibitors possessing antibacterial activity along with the ability to inhibit biofilms would be better therapeutic agents for diabetic patients. In the present study, two fractions (AF1 and AF2) possessing α-glucosidase inhibitory activity were purified from an endophytic fungus Alternaria destruens (AKL-3) isolated from Calotropis gigantea. These were evaluated for their antimicrobial and antibiofilm potential against human pathogens. AF1 exhibited broad spectrum antimicrobial activity against all the tested pathogens. It also significantly inhibited biofilm formation and dispersed the preformed biofilm at sub-optimal concentrations. AF2 possessed lesser activity as compared to AF1. The active compounds were purified using semi preparative HPLC. Some of the active compounds were identified to be phenolic in nature. The active fractions were also determined to be non-mutagenic and non-cytotoxic in safety analysis. The study highlights the role of endophytic fungi as sources of α-glucosidase inhibitors with antimicrobial potential which can have application in management of diabetes.
KeywordsEndophytes Alternaria destruens α-Glucosidase inhibitors Antibacterial Biofilm inhibition Biofilm dispersion
Brain heart infusion
Chinese hamster ovary
Fourier transform infrared spectroscopy
High performance liquid chromatography
(3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazolium bromide
Thin layer chromatography
Authors acknowledge the grant of fellowship under UPE (University with Potential for Excellence) scheme of University Grants Commission and Council of Scientific and Industrial Research (CSIR), New Delhi, India for providing financial assistance.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
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