Molecular Biology Reports

, Volume 46, Issue 6, pp 6495–6500 | Cite as

A potential marker in brucellosis, long non coding RNA IFNG-AS1

  • Reza Gheitasi
  • Sanaz Jourghasemi
  • Iraj Pakzad
  • Vahid Hosseinpour Sarmadi
  • Yazdan Samieipour
  • Zamberi Sekawi
  • Farid Azizi JalilianEmail author
Original Article


Brucellosis is the most common bacterial zoonotic infection. This pathogen may survive and sustain in host. The aim of this study is to define relationship between long noncoding (lnc) RNA-IFNG-AS1 and interferon gamma (IFN-γ) in different groups of patients with brucellosis compared to control group. In this study, associations of lncRNA IFNG-AS1 expression with secretion of IFN-γ level in Sixty patients with brucellosis, which were divided into 3 groups (acute, chronic and relapse groups), as a case group were compared with 20 subjects with negative serological tests and brucellosis clinical manifestation as a control group. In this regard, RNA were extracted from isolated peripheral blood mononuclear cells (PBMCs). LncRNA IFNG-AS1, T-box transcription factor (T-bet) and IFN-γ expressions were detected using quantitative polymerase chain reaction (qPCR). Serum level IFN-γ was assessed using enzyme linked immunosorbent assay (ELISA). The results showed that expression level of LncRNA IFNG-AS1, T-bet and IFN-γ increased significantly in all patient groups in compared to healthy subjects (P < 0.0001, P < 0.01, P < 0.001). However, there was no significant difference in T-bet expression between chronic and healthy groups (P = 0.98). Additionally, further analysis revealed that the serum level of IFN-γ in acute and relapsed groups were higher than control group (P < 0.0001, P < 0.001). The effective role of IFNG-AS1 in many protective actions, including enhancing the expression of INF-γ in the immune response of brucellosis patients, revealed new potential marker, LncRNA IFNG-AS1 in screening, diagnosis or treatment of brucellosis.


Brucellosis Cytokine Interferon-gamma T-bet lncRNA IFNG-AS1 



We would like to thank our colleagues in Clinical Microbiology Research Center, Ilam University of Medical Sciences for their encouragement, Prof. Nourkhoda Sadeghifard and Mrs Ali Hematian.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest to the publication of this article.

Ethics approval

This study was proved by Ilam University of Medical Sciences (No. IR_MEDILAM.REC.1397.064). All participants received questionnaire and written informed consent in accordance with ethical committee requirements.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Reza Gheitasi
    • 1
  • Sanaz Jourghasemi
    • 1
  • Iraj Pakzad
    • 2
  • Vahid Hosseinpour Sarmadi
    • 3
  • Yazdan Samieipour
    • 4
  • Zamberi Sekawi
    • 5
  • Farid Azizi Jalilian
    • 6
    Email author
  1. 1.Department of Immunology, School of MedicineHamadan University of Medical SciencesHamadanIran
  2. 2.Department Microbiology, Faculty of Medicine and Clinical Microbiology Research CenterIlam University of Medical SciencesIlamIran
  3. 3.Department of Immunology, Faculty of MedicineIran University of Medical SciencesTehranIran
  4. 4.Institute of VirologyTechnical University of MunichMunichGermany
  5. 5.Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health SciencesUniversity Putra MalaysiaSerdangMalaysia
  6. 6.Department of Medical Virology, Faculty of MedicineHamadan University of Medical SciencesHamadanIran

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