Markers of pancreatic cancer stem cells and their clinical and therapeutic implications
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Pancreatic cancer (PC) is the fourth most common cause of death among all cancers. Poor prognosis of PC may be caused by a prevalence of cancer stem cells (CSCs). CSCs are a population of cancer cells showing stem cell-like characteristics. CSCs have the ability to self-renew and may initiate tumorigenesis. PC CSCs express markers such as CD133, CD24, CD44, DCLK1, CXCR4, ESA, Oct4 and ABCB1. There is a wide complexity of interaction and relationships between CSC markers in PC. These markers are negative prognostic factors and are connected with tumor recurrence and clinical progression. Additionally, PC CSCs are resistant to treatment with gemcitabine. Thus, most current therapies for PC are ineffective. Numerous studies have shown, that targeting of these proteins may increase both disease-free and overall survival in PC.
KeywordsPancreatic cancer Cancer stem cells Prognostic factors
The diagnosis and treatment of pancreatic cancer (PC), which is the fourth most common cause of death among all cancers, is a current and important medical problem. The low survival rate for PC has been confirmed by data from 185 countries analyzed in the GLOBOCAN study of 2018, which clearly showed that the number of new cases of PC was similar to the number of deaths (458,918 vs. 432,242) . According to the WHO, the most common type of PC is adenocarcinoma . The main factor contributing to such a low survival rate is the diagnosis of PC in its advanced stages (52% for advanced stages vs. 9% for the initial stages) for whom the 5-year-survival rate is only 2–5%. The cancer mortality is caused predominantly by dysfunction of organs due to metastasis. This process is related to epithelial–mesenchymal transition (EMT), which is the transformation of cells from the epithelial to the mesenchymal-like phenotype. Cells which undergo EMT have increased motility, are more invasive, and are resistant to apoptosis . The most important contributory factors in PC are smoking (strong correlation) and the presence of Helicobacter pylori infection (moderate correlation), although diet and type 2 diabetes also play an important role [4, 5]. In addition, a recent study emphasized the role of chronic ethanol consumption on the transformation of normal human pancreatic ductal epithelial cells into altered cells with cancer stem cell phenotypes (CSCs) .
CSCs are a specific population of cancer cells showing stem-cell like characteristics and, according to increasing evidence, they may initiate the development of PC as is the case with other neoplasms   . CSCs seem to generate daughter cells through the process of cell division, which may occur next to ordinary tumor cells representing the major component of the tumor, or self-renew, maintaining the full ability to differentiate and divide just as the parent stem cell does  . The possibility of detecting specific markers identifying PC CSCs was investigated. There are several specific proteins that belong to this group, including CD133, CD24, CD44, CXCR4, EpCAM, Oct4, ABCB1, ABCG2, c-Met, ALDH-1, and nestin   . A unique feature of CSCs is their resistance to drugs. Without the elimination of this subpopulation during chemo- or radiotherapy of PC, it may induce tumor recurrence and subsequent clinical progression . This may be one of the reasons for the unsatisfactory outcomes in the cases of PC treatment .
Five-year-survival accounts for no more than 32%, even among patients who are diagnosed during the early stages of this disease. The classical approach to treatment is gemcitabine-based chemotherapy as the first line option, however this approach shows only limited benefit on the overall survival rate of patients with locally advanced or metastatic PC  . Currently, monotherapy with gemcitabine is recommended as an adjuvant treatment, in locally advanced cancer for 6 months, and as a palliative procedure among patients with ECOG (Eastern Cooperative Oncology Group)  performance scores of 2 and/or bilirubin levels higher than 1.5 × ULN . Fluorouracil (5-FU) with folinic acid, is another method used in addition to surgery . To improve the outcome in cases of advanced PC, new cycles of chemotherapy were investigated in individuals with this disease, which led to new regimens in clinical practice, such as FOLFIRINOX (Leucovorin + Fluorouracil + Irinotecan + Oxaliplatin) or nab-paclitaxel plus gemcitabine   . However, these regimens are recommended for palliative care, in cases of metastatic disease, only for patients with ECOG performance status scores of 0 or 1 and a bilirubin level below 1.5 × ULN, because of the increased toxic side effects . Nonetheless, the effectiveness of the new treatments remain limited.
The aim of this review was to summarize the most current and available information about the diverse subpopulation of CSCs in cases of PC, strictly in connection with their influence on the clinical features of the disease, prognostic features of specific CSC markers, and with the challenges related to the treatment process and behavior of these cells. Another aim was to collect data about the future possibility of targeting CSC markers in order to increase the efficacy of chemotherapy in PC.
Markers of cancer stem cells in pancreatic cancer as independent prognostic factors
CD24 is a small mucin-like glycosyl phosphatidylinositol (GPI)- linked cell surface protein involved in cell adhesion. It is present in the developing brain and kidneys [23, 24]. CD24 seems to be involved in the development of PC. Jacob et al. revealed diminished or absent expression of CD24 in normal pancreatic tissue and noticeable cytoplasmatic levels in PanIN (pancreatic intraepithelial neoplasia). Increasing expression of CD24 was observed in cells during the progression from normal ductal epithelium to invasive intraductal papillary mucinous carcinoma. Expression levels increased in line with increasing grade of atypia in intraductal papillary mucinous neoplasms. Smith et al. showed that CD24 expression is regulated by Ral GTPases. The Ral family initiates transcription through TCF downstream pathways and activation of transcription factors, such as Jun, NF-κB and AFX, which regulate the expression of CD24  . Moreover, Moghadam et al. observed more intensive oncogenic activity of the Ras-related protein Ral-A (RalA) in CD24+ cancer cells in comparison to CD24− cancer cells. Overexpression and increased RalA activity is predominant in PC and it is associated with tumorigenic growth  . CD24 also seems to be involved in integrin-associated cell to matrix adhesion and regulation of cellular morphology through stress fibers, processes of rounding, and nuclear condensation, and therefore protects cancer cells from apoptosis . In primary PC, CD24 was overexpressed in high-grade tumors and more advanced PC stages, suggesting its role in the progression of PC. According to Ikenaga et al. higher tumor stage, nodal metastasis, higher-grade tumors, microscopic lymphatic and venous and neural invasion was more frequently observed in their CD24+ group than in the group with CD24-negative PC. The shorter survival period was also associated with higher levels of CD24 expression, 12 months in the group with strong expressions, 19 in those with weak expression, and 23 in those with none [29, 30].
CD133 is a transmembrane protein that is found in lipid rafts, with a ganglioside-binding extracellular domain and a cytoplasmic domain, with the capability of tyrosine phosphorylation [31, 32, 33, 34]. The process of CD133 transcription is regulated by five promoters (P1, P2, P3, P4 and P5) . According many studies P5 seems to play crucial role by CD133 expression in PC CSCs. Its activation could be performed by a downstream signaling pathway initiated by one of the extracellular signal–regulated kinases (ERKs), mainly by ERK1 or ERK2 . In CSCs of PC ERKs are inducted by MAPK/ERK pathway triggered through the TrkA receptor in response to auto- or paracrine secreted Nerve growth factor (NGF) [36, 37, 38, 39]. On the other hand P5 appears to be a target for heat shock proteins such as HIF-1α and HIF-2α .Their expression increases during carcinogenesis in pancreatic tissue due to oxygen deficiency in the PC region, leading to increased CD133 transcription [40, 41, 42]. Other studies showed that Wnt/β-catenin signaling has been closely associated with regulating PC development and has promoted the self-renewal of CD133+ cancer cells [43, 44, 45]. Moreover, a study by Nagathihalli et al. showed that inhibition of Janus kinases (JAK) or Signal Transducer and Activator of Transcription protein 3 (STAT3) cause downregulation of CD133 + . Recent studies have revealed significantly lower expression of CD133 in normal pancreatic tissue (in under 0.01% of cancer cells) in regards to PC  . PCs are characterized by populations of 0.5–1% of CD133+ cells in less aggressive cell lines to more than 9% of CD133+ cells in clones with increased rates of migration . CD133 is a substrate for Src-family tyrosine kinases such as the proto-oncogene tyrosine-protein kinases Src and Fyn, which phosphorylate its cytoplasmic domain at tyrosine-828 and tyrosine-852 [31, 48].The result of this is the triggering of downstream regulatory signals for stemness properties (such as enhanced telomerase reverse transcriptase expression, increased Akt phosphorylation and ligand-independent EGFR activation) and EMT . EMT has been reported to be regulated in PC by the NF-κB signaling pathway, which is activated by CD133  . The overexpression of CD133 increases the expression and secretion of IL1β (IL1B) by CSCs, activating an autocrine signaling loop that upregulates NF-κB signaling in cancer cells . Studies have suggested that CD133 expression is significantly associated with metastases to the lymph nodes, clinical TNM stage and tumor differentiation in patients with PC  . Moreover, high CD133 expression has been described as an independent prognostic factor for lower disease-free survival . On the other hand, Durko et al. concluded that there was no significant influence of CD133 expression on overall patient survival .
The microtubule regulator DCLK1 marks a subpopulation of morphologically and functionally distinct pancreatic cells identified at the earliest stages of pancreatic tumorigenesis, which could function as CSC [56, 57, 58]. The upstream regulators of DCLK1 have not yet been satisfactorily investigated. The upregulation of DCLK1 could be promoted by the overexpression of immune cell-derived IL17 which is produced by mesenchymal inflammatory cells, increasing DCLK1 expression through activation of nuclear factor kB (NF-kB) via the canonical pathway to promote stemness . Overexpression of DCLK1 is responsible for the regulation of many intracellular pathways during malignant transformation of pancreatic tissue, mostly due to microRNA-related mechanisms [60, 61, 62]. It promotes tumorigenesis through the upregulation of important drivers of pancreatic tumorigenesis, such as cMyc and KRAS through a let-7a microRNA(let-7a)-dependent mechanism [62, 63, 64]. KRAS is mutated in more than 95% of all PC cases [65, 66]. The increasing level of DCLK1 cells could be an early event in KRAS-induced pancreatic tumorigenesis and its overexpression is significant related with presence of activated mutant Kras in PC tissues [58, 62, 67]. Another commonly overexpressed PC oncogene is epidermal growth factor receptor (EGFR) . According to the findings of Sureban et al. DCLK1 may upregulated by EGF signaling . It was reported that miR-145 and miR-143 inhibit cell proliferation by targeting EGFR in various types of cancers, [69, 70] and through their suppression, dependent on DCLK1, cell cycle arrest could be overcome. The NOTCH pathway (especially Notch-1) is a target of suppressor MicroRNAs, such as the miR-200 family (ZEB1 and miR-200) and the miR-144 family, whose deregulation could be caused by DCLK and lead to increased proliferative signaling in PC [61, 62, 71]. DCLK1 is also associated with the pluripotency of PC due to the repression of other tumor suppression factors including miR-143, miR-145 and let-7a, resulting in overexpression of pluripotency agents such as OCT4, SOX2, KLF4, NANOG and LIN28B [61, 62, 72, 73].It has also been reported that the influence of DCLK1 on apoptosis is due to the stimulation of 14-3-3 σ expression, which is an inhibitor of pro-apoptotic activity in PC [62, 74, 75]. DCLK1 induces deregulation of VEGF-inhibitors and leads to neovascularization of PC and, as a consequence, tumor growth [61, 76, 77, 78, 79, 80, 81]. Clinically, the DCLK1+ expression correlates with the histological grade and preoperative CA19-9 level . Furthermore, the overexpression of DCLK1 was observed in metastatic PCs, and the population of DCLK1+ cells in metastatic tissues was larger than that of primary tumors . DCLK1 may promote the metastatic capability of PC through its impact on EMT. One of the functions of the miR-200 family is the prevention of EMT in several cancers by inhibiting the transcription factors ZEB1 and ZEB2 [84, 85]. DCLK1 decreases the levels of these MicroRNAs and, in consequence, leads to the activation of processes by upregulation of EMT proteins such as Snail, Slug and Twist [61, 62]. The overexpression of DCLK1indirectly stimulates the oncogene BMI1 with upregulation of the Polycomb complex protein Bmi-1 expression in PC, which leads to the triggering of EMT through upregulation of mesenchymal markers such as Snail and Vimentin, as well as by downregulation of the epithelial marker E-cadherin . The study also found that DCLK1 is an unfavorable prognostic factor in PC, with median overall survival after resection being 18.7 or 49.5 months in tumors with positive and negative DCLK1 expression respectively. Additionally, the median disease free survival time has been found to be shorter among PCs with DCLK1 expression (10.2 months) in comparison to PCs without DCLK1 surface expression (25.5 months) .
Another important marker is CD44. It is a membrane receptor for hyaluronic acid (HA), osteopontin, chondroitin, collagen, fibronectin and serglycin/sulphated proteoglycan. Therefore, CD44 is involved in processes such as cellular adhesion, angiogenesis, the release of cytokines and muscle repair  .There is evidence that this protein might have an important role in the development of PC cancer. The CD44 gene is encoded by 20 exons. The standard CD44 isoform (CD44s) is composed of exons 1–5 and 16–20, thus it is called the constant form. The variant CD44 isoform (CD44v) consists of the middle exons, which can be spliced and assembled with the ten exons present in CD44s. Therefore thousands of CD44 isoforms could possibly be generated [87, 88, 89]. In healthy cells p53 binds to a noncanonical p53-binding sequence of the CD44 promoter, which inhibits its expression. It has been shown that loss-of-function mutations of p53 may be associated with up-regulation of CD44, leading to the promotion of tumor growth . The study of Li et al. showed increased CD44v and decreased CD44s expression in metastatic PC. Furthermore, CD44s and CD44v were found to be associated with metastases to the lymph nodes and liver.  . Probably, the role of CD44v in invasion is associated with its interaction with the oncogenic transcription factor c-Myc. c-Myc activates the MEK and Erk pathways which leads to increased antiapoptotic activity due to decreased Fas signalling  . Studies have shown that HA-CD44 binding also leads to upregulation of other transcription factors such as Nanog, Sox2 and Oct4. This interaction promotes miR-302 and miR-21 overexpression resulting in increased spheroid and clone formation, as well as cell growth/self-renewal in CD44vhigh cancer cells [93, 94, 95, 96]. High invasiveness and the increased ability to metastasise of CD44+ PC is also associated with EMT. Overexpression of CD44 leads to an increase in EMT-related mesenchymal cancer cell phenotypes. What is more, switching between CD44v and CD44s is crucial in EMT and the CD44s isoform seems to be the most prevalent in EMT [87, 97, 98]. A study by Brown et al. showed that CD44s upregulation correlated with the levels of mesenchymal markers and high grade of cancer. The authors also observed that CD44s activated the Akt pathway inhibiting E-cadherin expression and thus introducing EMT. According to their evidence, epithelial cell-type-specific splicing regulator (ESRP1) is connected with this process. It stimulates CD44 posttranscriptional changes and is commonly expressed in epithelial cells. ESRP1 knockdown in CD44v cells was found to be associated with switching to CD44s and to the suppression of metastasis. ESRP1 overexpression prevented cells from undergoing EMT by stimulating CD44 splicing, which led to high levels of CD44v and blocked the switch from CD44v to CD44s   . There are clinical consequences of these alternations and several studies have demonstrated the correlation between CD44 expression and poor prognosis in PC. CD44 overexpression has been correlated with advanced clinical stage and lymph metastases . Hong et al. revealed that median survival of patients with CD44 positive PC was shorter (20.3 months) than for patients with CD44 negative PC (25.3 months) .
CXCR4 is a G-protein-coupled receptor for stromal-derived-factor-1 (SDF-1). CXCR4 expression occurs mainly on hematopoietic cells but its presence can be found on other cells such as endothelial cells, stromal cells or on the surface of mature blood cells [103, 104]. CXCR4 and SDF-1 are currently playing an increasingly important role in the pathogenesis of PC. In the work of Koshiba et al. CXCR4 expression was shown to be present in most cases [105, 106, 107]. The expression of CXCR4 is regulated in healthy cells by Nuclear Respiratory Factor 1 (NRF1) and Ying Yang 1. NRF1is a second messengers of many cellular signaling pathway and growth factors promote transcription of CXCR4 whereas Ying Yang 1 and proinflammatory factors have the opposite effect CXCR4, by joining CXC12, is involved in the migration of immune cells within the bone marrow and lymph nodes [110, 111]. In the case of the neoplastic process, of PC, a similar principle may lead to metastasis. Expression of CXCR4 on the surface of tumor cells can direct the tumor metastases towards SDF-1 expressing tissues via SDF-1-CXCR4 axis [112, 113]. Other studies have suggested the involvement of CXCR4 in metastases as a result of increased NK cell apoptosis. A number of mechanisms have been proposed in which CXCR4 interacts with the tumor microenvironment. Burger and Kipps have presented the influence of tissue hypoxia on the expression of CXCL12 by fibroblasts which directly activates CXCR4 and, at the same time, stimulates the attraction of cancer cells that contain this receptor on their surface . Maréchal et al. suggested a positive correlation between high CXCR4 expression and a shorter 5-year overall survival and a greater risk of lymph node and liver metastases . One of the possible mechanisms by which CXCR4 may affect the development and progression of PC is its interaction with CXCL12, which may lead to increased angiogenesis and the formation of new blood and lymphatic vessels. In the study of Cui et al. a higher microlymphatic vessel density was found in patients with higher CXCR4 expression, but with no apparent effect on microvessel density, which was more dependent on higher CXCL12 expression .
Oct4 is a protein belonging to the POU family and is responsible for differentiation and pluripotency [117, 118]. Oct4 is a transcription factor, whose gene is located on chromosome 6 (6p21.31). This protein is the main factor in pluripotency, participating in cell differentiation, reprogramming and renewal . Oct4 fulfills its role by binding an octameric sequence motif (ATGCAAAT) which allows for the regulation of gene expression . In the initial stage of development, this protein is present in all parent pluripotent cells whereas in later stages its expression can be demonstrated in germinal stem cells . The highest expression of Oct4 occurs in undifferentiated cells and decreases as the cells are differentiated. Oct4, combining with other factors such as SOX2 and NANOG, participates not only in the regulation of gene expression responsible for cell renewal and differentiation but also affects the expression of other factors such as fibroblast growth factor 4 (FGF4) [122, 123]. Statistically significant increased expression of Oct4 can be found in metaplastic ducts (79.2%) compared to malignant (19.4%) and nonmalignant (16.7%) tissue and high Oct4 expression is correlated with tumor differentiation [124, 125]. In order to explain the role of Oct4 in pancreatic tumors, Lu et al. knocked down both Oct4 and Nanog, which contributed to a reduction in proliferation, migration and invasion of PC stem cells and to increased sensitivity to chemotherapy with gemcitabine. The probable mechanism, as postulated by the authors, is based on the regulation of Caspase-3 and Bcl-2 expression .
ESA (EpCAM) is a type I transmembrane glycoprotein expressed on most epithelia. A study by Maetzel et al. showed that EpCAM intracellular domains form a complex with β-catenin and LEF-1 which activates the transcriptions of genes such as c-Myc and cyclins A and E. Its overexpression increased the phosphorylation of AKT, mTOR, p70S6K and 4EBP1 as well as decreased PTEN expression. EpCAM regulates EMT and metastasis in vivo  . The study of Fong et al. revealed that ESA was overexpressed in PC and that it was related to shorter survival of patients with advanced PC. Patients with advanced PC and ESA overexpression had an overall survival period of 48 months, whereas without this marker, the period was more than 70 months .
ABCB1 (MDR1 P-glycoprotein) is a member of the ABC superfamily of drug transporters, and is involved in the resistance of pancreatic cells due to increased drug efflux . Histopathological studies indicate that 63% of pancreatic tumors express glycoprotein, which is related to multidrug resistance of these cancers . ABCB1 is a transcriptional targets of WNT/β-catenin signaling and, in the case of gain-of-function CTNNB1 mutations, leads to overexpression of ABCB1 in PC . Moreover, transcription of ABCB1 is also increased by the activation of cells by leptins in the PC microenvironment . It has been reported that microRNA-21, detected in PC tissue, promotes upregulation of ABCB1 in cancer cells [134, 135, 136]. ABCB1 expression is directly activated by the demethylation of CpG islands in the ABCB1/MDR1 promoter region . Patients with high ABCB1 immunostaining had a shorter postoperative survival time (7.5 months) when compared with patients with weak to moderate expression of this protein .
Co-expression of CSC markers in pancreatic cancer
CSC as a cause of resistance to standard chemotherapy in pancreatic cancer
CD133+ cells show increased expression of anti-apoptotic genes such as Bcl-2 . Another study suggested that the reoccurrence of PC after treatment with gemcitabine was composed of mainly CD44+ cells, compared to the number of CD133+ and ESA+ cells . This observation add weight to the previously described supposition that CD44 not only upregulates cancer cell survival pathways, but also accelerates the proliferation of CSC. In summary, clinical studies in genetics suggest that CD44+ cells are able to proliferate at a faster rate, which enables their dominancy in tumor recurrence. Not only gemcitabine, but also other standard anticancer drugs are ineffective in PC with CSCs. Studies revealed that CD133+ cells also seem to be resistant to 5FU and nab-paclitaxel, which only reduce the population of CD133− cells but without having any effect on CD133+ cells [42, 47]. These effects seems to be caused by ABC transporters, which have the ability, in the case of CD133+ cells, to dispose not only of Gemcitabine, as is the case with CD44+ cells, but also Paclitaxel and 5FU . To explain why this phenomenon has been observed only in DC133+ cells requires additional studies, however.
The molecular observations described above may be suitable for use in clinical practice. A retrospective multi-center study including 1056 patients with metastatic PC showed that the median overall survival is dependent on the palliative chemotherapeutic cycle used, and on the use of FOLFIRINOX for up to 9.9 months, nab-paclitaxel with gemcitabine for 7.9 months, and 4.9 months of gemcitabine monotherapy . Similarly, an adjuvant treatment study among a population of 493 patients, by Conroy et al. showed that this kind of therapy with a FOLFIRINOX regimen led to significantly longer survival (54.4 months) than gemcitabine (35.0 months) . Furthermore, the overall survival rate at 3 years was 63.4% in the modified-FOLFIRINOX group and 48.6% in the gemcitabine group. These results could be explained by the tendency of CSCs, and above all CD44+ CSCs, to be insensitive to gemcitabine treatment. The visibly shorter survival period of patients who were treated with this drug, in comparison to remaining options, may be associated with the drug related selection of CD44+ cells and their unlimited proliferation. In the case of combined nab-paclitaxel and gemcitabine treatment, the longer survival rate in comparison to gemcitabine alone might arise from the sensitivity of CD44+ cells to nab-paclitaxel, whose proliferation is stopped with this drug. However, given that the PC resistance to gemcitabine might be explained by well described CSCs features, in a mostly CD44+ subpopulation, the sensitivity of these cells population to FOLFIRINOX needs further studies. The progression of disease during chemotherapeutic treatment may be the result of CD133+ proliferation, which could indicate resistance to both nab-paclitaxel and gemcitabine, and could become dominant in the CSC population, while CD44+ cells are eliminated.
New possibility of pancreatic cancer treatment based on CSC-targeting factors
The relationship between PC chemotherapy resistance and CSC markers described above leads us to conclude that the targeting of these proteins may increase both disease-free and overall survival periods for patients with this neoplasm. A study revealed that CD24 monoclonal antibodies inhibited the growth of human high CD24+ PCs in relation to both time and dose of exposure . Furthermore, the group’s next study showed that anti-CD24 antibodies might enhance the effects of oxaloplatin, 5-fluorouracil, doxorubicin, irinotecan, and paclitaxel on the reduction of CD24+ cells . Moreover, Shah et al. observed that CD24+ , CD44+ , and ESA+ cells were more invasive and exhibited increased activation of c-Met, which is one of the potential CSC targets . The c-Met inhibitor with gemcitabine reduced the population of CD24+ , CD44+ , and ESA+ cells, decreased tumor sphere formation in these cells and prevented the growth of the tumor for 6 weeks after the therapy. The authors suggested that the combined use of c-Met inhibitor and gemcitabine might be an effective treatment of PC, due to effects on CSC and bulk PC cell populations . Furthermore, the research of Mueller et al. showed that a combination of cyclopamine/CUR, rapamycin, and gemcitabine may cause tumor regression and abolishes CD24+ CD44+ ESA+ and CD133+ CSC subpopulations with a better survival rate after a 100 days of observation in comparison to gemcitabine only . Moreover, their studies suggested that metformin could inhibit the proliferative activity of CD133+ PC cells leading to decreased pancreatic tumor weights by as much as 34–49% according to drug concentration [54, 171, 172]. On the other hand, the influence of metformin did not affect PC stem cells with other CSC markers, such as CD24+ , CD44+ , ESA+ and co-expression of them . Gupta et al. also showed significantly lower tumor volume among PCs with an initially high population of CD133+ cells (10–25% of cells) in mice treated with lovastatin in combination with paclitaxel when compared to a control group, which suggests that lovastatin may sensitize these cells to paclitaxel . In the Rangarajan et al. study it was found that crocetinic acid suppressed the growth of the tumor and the expression of CD133 and DCLK1 . Another interesting result observed during a study with the kinase inhibitor XMD8-92, showed that injecting this drug caused a reduction in xenograft volume and weight, as well as significant downregulation of DCLK1 expression . In a recent study, significantly reduced cell survival in cases of combined treatment of PC with gemcitabine and DCLK1 inhibitor was demonstrated, compared to individual treatment with gemcitabine in a cell line model . Interestingly, a study by Cioffi et al. described finding that EpCAM/CD3-Bispecific T cell Engaging Antibody MT110 may eliminate primary human pancreatic CSCs in vivo and in vitro, due to the redirection of cytotoxic T lymphocytes against human pancreatic CSCs. As a result, it induced apoptosis, inhibited sphere formation and reduced tumorigenicity of PC in mice. Additionally, the authors observed that the addition of gemcitabine caused no further reduction of tumor size . Another substance, namely acetyl-11-keto-b-boswellic, could be useful in the suppression of progression in CXCR4+ cancer due to downregulation of the CXCR4 receptor and by reduction of the invasiveness of PC . On the other hand, Wang and al. have shown that a blocking ligand binding to CXCR4 had positive effects, not only by reducing PC growth, but also by increasing the sub-G1 apoptotic compartment, thus affecting the G0–G1 phase and, by extension, slowing the G2 and S phases . Moreover, because knockdown of CXCR4 and OCT4 decreases the invasiveness of PC, these factors may also be a promising point in the future of PC therapy   Equally interesting are the results of Gao et al. in which it was shown that there is less microRNA-335 to be found in OTC4 (+) PC cells than in OCT4 (−) cells and that miR-335 may cause OCT4 suppression, which in turn results in slower development and decreased tumor expansion .
Cancer stem cells in pancreatic cancer
Resistance to gemcitarabine
Role in PC
Features of PC with high expression
– Integrin-associated cell to matrix adhesion
– Regulation of cellular morphology
– Protection from apoptosis 
– High-grade tumors
– Advanced pT stages
– Nodal metastasis
Cyclopamina/CUR, rapamycin, gemcitabine—reduction of CD24+ PC cell in vivo and in vitro 
– Upregulation of EMT
– Gaining the properties of stemness
– Autocrine signaling
– Lymph node metastasis
– Tumor differentiation
Cyclopamina/CUR, rapamycin, gemcitabine—reduction of CD133+ PC cell in vivo and in vitro ;
Lovastatin, paclitaxel—lower tumor volume in PCs with high population of CD133+ cells in mice ; metformin—inhibition of the proliferation of CD133+ CSCs in clinical trials ; crocetinic acid—inhibition of PC in vivo 
– Upregulation c-myc and KRAS
– Suppression of miR-145 and miR-143, overexpression of EGRF
– Increased proliferative signaling
– Repression of s miR-143 and miR-145 and let-7a, overexpression of pluripotency agents OCT4, SOX2, KLF4, NANOG and LIN28B
– Antiapoptotic activity
– Downregulation of VEGF-inhibitors, neovascularization
– Histological grade
– Preoperative CA19-9
– Lymph node metastasis, advanced clinical stage 
Cyclopamina/CUR, rapamycin, gemcitabine—reduction of CD44+ PC cell in vivo and in vitro 
– Interaction with CXCL12
– Increased angiogenesis and formation of new blood and lymphatic vessels 
– Lymph node and liver metastases 
Acetyl-11-keto-b-boswellic—suppression of PC cells in vivo 
– Forming a complex with β-catenin and LEF-1
– Activation transcriptions of genes such as c-Myc, cyclins A and E
– Increased phosphorylation of AKT, mTOR, p70S6K and 4EBP,
– Decreased PTEN expression
– Regulation of EMT and metastasis
Cyclopamina/CUR, rapamycin, gemcitabine—reduction of ESA + PC cell in vivo and in vitro ;
MT110 elimination of pancreatic CSCs in vivo and in vitro ;
– Drug efflux 
Compliance with the ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
For this type of study formal consent is not required.
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