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Molecular Biology Reports

, Volume 46, Issue 6, pp 5821–5829 | Cite as

Analysis of VEGF gene polymorphisms and serum VEGF protein levels contribution in polycystic ovary syndrome of patients

  • Lei Bao
  • Rabbani Syed
  • Mustafa Sawsan AloahdEmail author
Original Article

Abstract

Vascular endothelial growth factor (VEGF) is a well-known factor in reproductive function and contributes to the pathogenesis of polycystic ovary syndrome (PCOS). Genetic variations in VEGFA gene were suggested to contribute alterations in VEGF secretion and PCOS. This study evaluated the association of VEGFA SNPs with altered VEGF secretion level and PCOS among ethnically-matched control women. This prospective case–control study was conducted from 2016 to 2018 and comprised of 55 women with PCOS and 52 control subjects. ELISA was used to measure VEGF levels; and various other related bio chemicals whereas the genotyping of VEGFA variants was performed through the analysis of nine SNPs of VEGF. PRL, E2, PRGE testosterone and glucose level were found to be insignificantly different. The levels of FSH, LH, LH/FSH, TT, insulin, SHBG and HOMA-IR were significantly higher in the study group. Among the nine tested variants of VEGF SNPs, two SNPs rs3025020 and rs833061, consisted of TT (Recessive and Dominant homozygous, respectively) which were marginally higher in test. The SNP rs1570360 had significantly higher GG allele (32.73%) which was recessive homozygous. There was no significant difference observed in genotype frequencies related to higher value of VEGF. The genotype frequencies for the studied SNPs were in alignment with Hardy–Weinberg equilibrium (HWE). The mean serum VEGF levels got significantly increased in PCOS group. No significant association was found between VEGF genotypes and its serum levels. VEGF levels in rs699947 (AA-major homozygous), rs3025039 (CC-major homozygous) and rs833061 (TT & CC-major & minor homozygous) genotypes were significantly higher in PCOS. The study results evidently proved that the allelic variants in genes may be a factor for PCOS and VEGF serum levels with respect to few SNP variants only. These findings indicated that VEGF may be involved in PCOS status and confirmed the previous association between genetic variants in VEGF, serum level of VEGF protein and PCOS.

Keywords

Vascular endothelial growth factor (VEGF) Polycystic ovary syndrome (PCOS) genetic variation Single nucleotide polymorphism Serum VEGF levels 

Notes

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Informed consent

Written informed consent was obtained from the participants and in case of the minor study participant, informed consent was granted from the legally authorized representatives of the minors with the option to withdraw them from the study at any time.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.The International Peace Maternity and Child Health Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Shanghai Key Laboratory of Embryo Original DiseasesShanghaiChina
  3. 3.Shanghai Municipal Key Clinical SpecialtyShanghaiChina
  4. 4.Department of Pharmaceutics, College of PharmacyKing Saud UniversityRiyadhSaudi Arabia
  5. 5.College of Life ScienceMaulana Azad College of Arts and ScienceAurangabadIndia

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