Evaluation of urinary 8-hydroxy-2-deoxyguanosine level in experimental Alzheimer’s disease: Impact of carvacrol nanoparticles
The present study aimed to compare the effect of carvacrol essential oil and carvacrol nanoemulsion against experimental Alzheimer’s (AD). Forty male albino rats were used and divided into four groups as follow: control, AlCl3 induced AD, carvacrol oil treated and carvacrol nanoemulsion treated groups. Brain nor-epinephrine, serotonin and dopamine were analyzed by high performance liquid chromatography (HPLC). Levels of brain Thiobarbituric acid-reactive substances (TBARS), Superoxide dismutase (SOD), reduced glutathione (GSH), cholinesterase, and advanced oxidation protein product (AOPP) were evaluated. Urinary 8-hydroxyguanosine (8-OHdG) level was evaluated by HPLC. Brain Cyclooxygenase 1 and 2 (COX 1and 2) were analyzed by immunohistochemistry. AD induced by AlCl3 in rats was depicted by the significant increase in the neurotransmitters levels which is accompanied with high degree of oxidative stress that was revealed in the elevated level of urinary 8-OHdG along with significant elevation in AOPP, TBARS, and cholinesterase levels and a significant decrease in SOD and GSH; these results are confirmed by immunohistochemistry analysis of COX 1 and 2. On the other hand, the treatment with carvacrol oil and carvacrol nanoemulsion were capable of mitigate effects mediated by AlCl3 administration in treated rats. While the treatment with both approached succeeded to retract the negative impact of AlCl3; but the effect of carvacrol nanoemulsion was more notable than the essential oil. Carvacrol oil and carvacrol nanoemulsion were eminent to overturn AlCl3 induced brain AD which could be imputed to antioxidant and anti-inflammatory capabilities of carvacrol to alter oxidative stress effect. In extension; carvacrol nanoemulsion were evident to give more effective and efficient way in carvacrol delivery to pass through blood brain barriers and ameliorate brain changes.
KeywordsAlzheimer’s disease 8-hydroxy-2-deoxyguanosine Neurotransmitters Advanced oxidation protein product Cyclooxygenases Carvacrol nanoemulsion
Authors are grateful to National Research Centre, Egypt for supporting this work.
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Compliance with ethical standards
Conflict of interest
The Authors declares that there is no conflict of interest.
- 2.Korolev IO (2014) Alzheimer’s disease: a clinical and basic science review. Med Stud Res J 4:24–33Google Scholar
- 4.Tan ZS, Spartano NL, Beiser AS, DeCarli C, Auerbach SH, Vasan RS, Seshadri S (2016) Physical activity, brain volume, and dementia risk: the Framingham study. J Gerontol Ser A 72(6):789–795Google Scholar
- 7.Padurariu M, Ciobica A, Lefter R, Lacramioara Serban I, Stefanescu C, Chirita R (2013) The oxidative stress hypothesis in Alzheimer’s disease. Psychiatr Danub 25(4):0–409Google Scholar
- 11.Kawahara M, Kato-Negishi M (2011) Link between aluminum and the pathogenesis of Alzheimer’s disease: the integration of the aluminum and amyloid cascade hypotheses. Int J Alzheimer’s Dis 2011:276393Google Scholar
- 21.Hussein J, Refaat E, Morsy S, Medhat D, Oraby F (2013) Green tea attenuates experimental hepatitis in context of oxidative stress. J Appl Pharm Sci 3(12):124Google Scholar
- 22.Mannaa F, Ahmed HH, Estefan SF, Sharaf HA, Eskander EF (2005) Saccharomyces cerevisiae intervention for relieving flutamide-induced hepatotoxicity in male rats. Die Pharm Int J Pharm Sci 60(9):689–695Google Scholar
- 27.Hussein J, El-Khayat Z, Abdel Latif Y, Medhat D, Morsy S, Oraby F (2014) Evaluation of brain monoamines in diabetic rats treated with quercetin. J Chem Pharm Res 6(10):384–390Google Scholar
- 28.Hussein J, Abo Elmatty D, Medhat D, Mesbah N, Abdel Razik F, Fahmy H (2016) Flaxseed oil attenuates experimental liver hepatitis. Der Pharm Lett 8(8):142–150Google Scholar
- 32.Suganthi RU, Manpal S (2013) Biological and pharmacological of actions carvacrol and its effects on poultry: an updated review. World J Pharm Pharm Sci 2(2013):3581–3595Google Scholar
- 33.Medhat D, Hussein J, El-Naggar ME, Attia MF, Anwar M, Latif YA, Booles HF, Morsy S, Farrag AR, Khalil WK, El-Khayat Z (2017) Effect of Au-dextran NPs as anti-tumor agent against EAC and solid tumor in mice by biochemical evaluations and histopathological investigations. Biomed Pharmacother 91:1006–1016CrossRefPubMedGoogle Scholar
- 38.Moustafa YM, Medhat D, Zaitone SA, Ei-Khayat Z, Abdel-Salam OM, Abdalla AA (2018) Amyloid beta-peptide (1–42) induced neurotoxicity in experimental rats: effect of Donepezil. Biosci Res 15(3):1931–1942Google Scholar
- 40.Pocernich CB (1822) Butterfield DA (2012) Elevation of glutathione as a therapeutic strategy in Alzheimer disease. Biochim et Biophys Acta 5:625–630Google Scholar
- 44.Oksidatif KKY, Stres N, Derleme EB (2006) Oxidative/nitrosative stress in chronic heart failure: a critical review. Türk Biyokimya Dergisi 31(2):86–95Google Scholar
- 46.Isobe C, Abe T, Terayama Y (2010) Levels of reduced and oxidized coenzyme Q-10 and 8-hydroxy-2′-deoxyguanosine in the CSF of patients with Alzheimer’s disease demonstrate that mitochondrial oxidative damage and/or oxidative DNA damage contributes to the neurodegenerative process. J Neurol 257(3):399–404CrossRefPubMedGoogle Scholar