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Molecular Biology Reports

, Volume 46, Issue 4, pp 4437–4441 | Cite as

Addressing the impact of different fetal bovine serum percentages on mesenchymal stem cells biological performance

  • Ramada R. KhasawnehEmail author
  • Ahmed Hesham Al Sharie
  • Ejlal Abu-El Rub
  • Abdullah Omar Serhan
  • Hayam Nizar Obeidat
Original Article

Abstract

Human mesenchymal stem cells (MSCs) are presently on the top of hierarchy in the field of stem cell therapy, due to their miraculous therapeutic abilities in diminishing the symptoms of many chronic diseases and initiating regeneration and repair for various damaged tissues and organs. The foremost initial step to reach high success rate in any MSCs based study is the optimization of culture growth media by establishing a suitable fetal bovine serum (FBS) percentage that suits the purpose of MSCs based experiment. Choosing the suitable FBS percentage is a controversial issue and merely depends on the researchers experience and suggested recommendations by the suppliers. Despite the huge improvements in overall MSCs investigating approaches, there are no definite protocols that set up a range of FBS percentages that can be followed. Toward achieving this objective, we evaluate in the present report the effect of using various FBS percentages (5–20%) added to DMEM low glucose media, on the biological behaviour of MSCs. Growing MSCs in high FBS percentages containing culture media (15% and 20% FBS) increase the proliferation and expansion rate of MSCs, although it decreases the immunosuppressive properties. On the other hand, adding low FBS percentage (7% FBS) to MSCs culture media enhanced the immunosuppression characteristics of MSCs, even though the proliferation rate was moderately reduced. 7% FBS is the cut off percentage that can be used without negatively altering major MSCs biological properties in which using 5% FBS will cause a tremendous decrease in the proliferation capacity and immunosuppressive properties. This report may assist other researchers in choosing appropriate FBS percentage when preparing MSCs culture media that serve the purpose of their MSCs based studies.

Keywords

Human BM-MSCs Fetal bovine serum (FBS) Passages IL-10 IDO Proliferation Immunosuppression 

Notes

Acknowledgements

This study was funded by a grant from the deanship of Research at Yarmouk University.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Basic Medical Sciences, Faculty of MedicineYarmouk UniversityIrbidJordan
  2. 2.Faculty of MedicineJordan University of Science and TechnologyIrbidJordan
  3. 3.Department of Physiology and PathophysiologyUniversity of ManitobaWinnipegCanada

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