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Molecular Biology Reports

, Volume 46, Issue 4, pp 4409–4421 | Cite as

Maize transcriptomic repertoires respond to gibberellin stimulation

  • Yali Wang
  • Xin Wang
  • Dexiang Deng
  • Yijun WangEmail author
Original Article

Abstract

Phytohormone gibberellin (GA) serves as hub modulator of diverse biological events. Understanding the transcriptomic features of GA-mediated processes has scientific significance. The transcriptomic landscapes of cereal crops upon GA stimulation remains largely unknown. Herein, to reveal the transcriptomic changes in cereal crop maize under GA treatment, we first selected normal height and GA-sensitive maize dwarf plants from advanced backcross population for GA treatment. RNA-seq analysis discovered multiple protein-coding transcripts that were differentially expressed in GA-treated samples compared to distilled water-treated ones. Some differentially expressed transcripts, namely GA-responsive transcripts in this study, encoded the components of GA pathway, including CPS, KS, and KO enzymes for GA biosynthesis, GA2ox enzymes for GA degradation, DELLA repressors and GID1 receptor for GA signaling. A total of 214 shared GA-responsive transcripts were identified both in GA3-treated normal height and GA-sensitive dwarf samples. Shared GA-responsive transcripts were involved in GA signaling, auxin biosynthesis, ethylene response, the composition and structure of cell wall, chlorophyll biogenesis, and sugar homeostasis. In addition, the convergence and divergence in expression of shared GA-responsive transcripts were observed in GA3-treated normal height and GA-sensitive dwarf plants. Interaction network modeling indicated that some shared GA-responsive transcripts tended to be co-regulated, which increases the complexity of GA-triggered regulation at transcriptomic layer. Results presented here will extend our knowledge of GA-mediated regulatory cascade, and enhance our ability to apply hormone GA knowledge in agricultural practice.

Keywords

Gibberellin Transcriptomic dynamics Interaction network Advanced backcross population Maize (Zea mays L.) 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31571671), the National Key Research and Development Program of China (2016YFD0101002), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (18KJA210002), the High-end Talents Support Plan of Yangzhou University (18HTYZU12), the Qing Lan Project of Yangzhou University (QLYZU201809), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11033_2019_4896_MOESM1_ESM.png (80 kb)
Supplementary material 1 (PNG 79 kb). Fig. S1 GO enrichment analysis of GA-responsive transcripts in GA3–treated WT. The 10 most enriched GO terms in each ontology are shown
11033_2019_4896_MOESM2_ESM.png (80 kb)
Supplementary material 2 (PNG 80 kb). Fig. S2 GO enrichment analysis of GA-responsive transcripts in GA3–treated D11. The 10 most enriched GO terms in each ontology are shown
11033_2019_4896_MOESM3_ESM.docx (15 kb)
Supplementary material 3 (DOCX 15 kb)
11033_2019_4896_MOESM4_ESM.xlsx (3 mb)
Supplementary material 4 (XLSX 3031 kb). Table S2 Information of expressed protein-coding transcripts in WT and D11 under distilled water and GA3 treatment
11033_2019_4896_MOESM5_ESM.xlsx (583 kb)
Supplementary material 5 (XLSX 583 kb). Table S3 Information of GA-responsive transcripts with different expression pattern
11033_2019_4896_MOESM6_ESM.xlsx (15 kb)
Supplementary material 6 (XLSX 15 kb). Table S4 Information of shared GA-responsive transcripts between GA3-treated WT and D11

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular BreedingAgricultural College of Yangzhou UniversityYangzhouChina
  2. 2.Jiangsu Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhouChina
  3. 3.College of AgricultureAnhui Science and Technology UniversityFengyangChina

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