A simple and effective method to obtain high DNA quality and quantity from Cerrado plant species

  • Diego Cerveira de SouzaEmail author
  • Terezinha Aparecida Teixeira
Short Communication


Despite the importance in conservation and breeding purposes, molecular studies using Cerrado plant species are still rare, mainly because of their high amounts of secondary compounds, impeding DNA extraction to downstream applications, such as PCR amplification. To date, the DNA extraction methods described are sometimes inadequate for these species, expensive, time-intensive and/or use very toxic reagents. Here, we present a simple and effective method, based on SDS and Triton X-100, to obtain high DNA quality and quantity from Cerrado plant species for molecular biological techniques. The DNA obtained by our protocol was free of contaminants and excellent for enzymatic restriction and PCR amplification. The concentration of extracted DNA across all tested species ranged from 156 to 1166 ng µL−1 (1.56–11.66 µg g−1 of dry tissue), with an A260/A280 ratio from 1.78 to 1.92. The new DNA extraction protocol described here provides high DNA quality and quantity from Cerrado plant species in a fast, simple and less toxic way. Thus, the use of our method will allow ecologists, geneticists and breeders to rapidly obtain high-quality and -quantity DNA from Cerrado plant species for any molecular biology study.


DNA extraction SDS Triton X-100 PCR amplification Enzymatic restriction 



  1. 1.
    Durigan G, Ratter JA (2016) The need for a consistent fire policy for Cerrado conservation. J Appl Ecol 53:11–15CrossRefGoogle Scholar
  2. 2.
    Klink CA (2013) Policy intervention in the Cerrado savannas of Brazil: changes in land-use and effects on conservation. In: Consorte-Mccrea AG, Santos EF (eds) Ecology and conservation of the maned wolf: multidisciplinary perspectives. CRC Press Taylor & Francis Group, Boca Raton, pp 293–308CrossRefGoogle Scholar
  3. 3.
    Strassburg BBN, Brooks T, Feltran-Barbieri R et al (2017) Moment of truth for the Cerrado hotspot. Nat Ecol Evol 1:1–3CrossRefGoogle Scholar
  4. 4.
    Mittermeier CG, Turner WR, Larsen FW, Brooks TM, Gascon C (2011) Global biodiversity conservation: the critical role of hotspots. In: Zachos FE, Habel JC (eds) Biodiversity hotspots: distribution and protection of priority conservation areas. Springer, Berlin, pp 3–22CrossRefGoogle Scholar
  5. 5.
    Silva MN (2010) Extração de DNA genômico de tecidos foliares maduros de espécies nativas do cerrado. Rev Arvore 34(6):973–978CrossRefGoogle Scholar
  6. 6.
    Moreira PA, Oliveira DA (2011) Leaf age affects the quality of DNA extracted from Dimorphandra mollis (Fabaceae), a tropical tree species from the Cerrado region of Brazil. Genet Mol Res 10(1):353–358CrossRefGoogle Scholar
  7. 7.
    Bressan EA, Rossi ML, Gerald LTS, Figueira A (2014) Extraction of high-quality DNA from ethanol-preserved tropical plant tissues. BMC Res Notes 7:268CrossRefGoogle Scholar
  8. 8.
    Barzegari A, Vahed SZ, Atashpaz S, Khani S, Omidi Y (2010) Rapid and simple methodology for isolation of high quality genomic DNA from coniferous tissues (Taxus baccata). Mol Biol Rep 37:833–837CrossRefGoogle Scholar
  9. 9.
    Dang PM, Chen CY (2013) Modified method for combined DNA and RNA isolation from peanut and other oil seeds. Mol Biol Rep 40:1563–1568CrossRefGoogle Scholar
  10. 10.
    Raimundo J, Reis CMG, Ribeiro MM (2018) Rapid, simple and potentially universal method for DNA extraction from Opuntia spp. fresh cladode tissues suitable for PCR amplification. Mol Biol Rep 45(5):1405–1412CrossRefGoogle Scholar
  11. 11.
    Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissues. Phytochem Bull 19:11–15Google Scholar
  12. 12.
    Tamari F, Hinkley CS, Ramprashad N (2013) A comparison of DNA extraction methods using Petunia hybrida tissues. J Biomol Tech 24:113–118Google Scholar
  13. 13.
    Kotchoni SO, Gachomo EW (2009) A rapid and hazardous reagent free protocol for genomic DNA extraction suitable for genetic studies in plants. Mol Biol Rep 36:1633–1636CrossRefGoogle Scholar
  14. 14.
    Ahmed I, Islam M, Arshad W, Mannan A, Ahmad W, Mirza B (2009) High-quality plant DNA extraction for PCR: an easy approach. J Appl Genet 50(2):105–107CrossRefGoogle Scholar
  15. 15.
    Faleiro FG, Faleiro ASG, Cordeiro MCR, Karia CT (2003) Metodologia para operacionalizar a extração de DNA de espécies nativas do Cerrado visando a análises moleculares. Embrapa, PlanaltinaGoogle Scholar
  16. 16.
    White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322Google Scholar
  17. 17.
    Martínez-González CR, Ramírez-Mendoza R, Jiménez-Ramírez J, Gallegos-Vázquez C, Luna-Veja I (2017) Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae). Plant Methods. Google Scholar
  18. 18.
    Healey A, Furtado A, Cooper T, Henry RJ (2014) Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species. Plant Methods. Google Scholar
  19. 19.
    Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1(4):19–21CrossRefGoogle Scholar
  20. 20.
    Jhala VM, Mandaliya VB, Thaker V (2015) Simple and efficient protocol for RNA and DNA extraction from rice (Oryza sativa L.) for downstream applications. Int Res J Biol Sci 4(2):62–67Google Scholar
  21. 21.
    Fang GH, Hammar S, Grumet R (1992) A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques 13(1):52–56CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Diego Cerveira de Souza
    • 1
    Email author
  • Terezinha Aparecida Teixeira
    • 1
  1. 1.Laboratory of Molecular GeneticsFederal University of Uberlandia (UFU)Patos de MinasBrazil

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