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Molecular Biology Reports

, Volume 46, Issue 4, pp 3777–3789 | Cite as

Comparative transcriptome profiling of resistant and susceptible sugarcane genotypes in response to the airborne pathogen Fusarium verticillioides

  • Zeping Wang
  • Yijie Li
  • Changning Li
  • Xiupeng Song
  • Jingchao Lei
  • Yijing Gao
  • Qiang LiangEmail author
Original Article

Abstract

Fusarium verticillioides is the pathogen associated with pokkah boeng disease (PBD), the most significant airborne disease of sugarcane. The molecular mechanisms that regulate the defense responses of sugarcane towards this fungus are not yet fully known. Samples of ‘YT 94/128’ (resistant, R) and ‘GT 37’ (susceptible, S) inoculated with F. verticillioides on the 14 days post-inoculation were used to analyze the transcriptome to screen R genes. In total, 80.93 Gb of data and 76,175 Unigenes were obtained after assembling the sequencing data, and comparisons of Unigenes with NR, Swiss-prot, KOG, and KEGG databases confirmed 42,451 Unigenes. The analysis of differentially expression genes (DEGs) in each sample revealed 9092 DEGs in ‘YT 94/128,’ including 8131 up-regulated DEGs and 961 down-regulated DEGs; there were 9829 DEGs in ‘GT 37,’ including 7552 up-regulated DEGs and 2277 down-regulated DEGs. The identified DEGs were mainly involved in catalytic enzyme activity, cell protease, hydrolytic enzymes, peptide enzyme, protein metabolism process of negative regulation, phenylpropanoid metabolism, extracellular region, aldehyde dehydrogenase, endopeptidase, REDOX enzyme, protein kinases, and phosphoric acid transferase categories. KEGG pathway clustering analysis showed that the DEGs involved in resistance were significantly related to metabolic pathways of phenylpropanoid biosynthesis, cutin, suberine and wax biosynthesis, nitrogenous metabolism, biosynthesis of secondary metabolites, and plant-pathogen interactions. This application of transcriptomic data clarifies the mechanism of interactions between sugarcane and F. verticillioides, which can help to reveal disease-related metabolic pathways, molecular regulatory networks, and key genes involved in sugarcane responses to F. verticillioides.

Keywords

Sugarcane Pokkah boeng disease Fusarium verticillioides Transcriptome Infection 

Notes

Acknowledgements

This research was supported jointly by National Natural Science Foundation of China (31801422), Natural Science Foundation of Guangxi Province (2018GXNSFAA281213, 2016GXNSFBA380046), Natural Science Foundation of Guangxi Province (2016GXNSFAA380010).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11033_2019_4820_MOESM1_ESM.xls (51.6 mb)
Supplementary material 1 (XLS 52,857 kb)

References

  1. 1.
    Luo T, Duan WX, Huang YZ, Tang SY, Wang ZP, Li YJ, Liu PW, Lin SH (2017) Occurrence of sugarcane pokkah boeng in sugarcane planting areas in cities of Liuzhou and Laibin, Guangxi in 2016 and variety resistance analysis. J South Agric 48(02):292–296Google Scholar
  2. 2.
    Siti NMS, Nur AIMZ, Azmi AR, Salleh B (2008) Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in peninsular malaysia. IEEE J Quant Electron 25(5):965–975Google Scholar
  3. 3.
    Lin ZY, Xu SQ, Que YX, Wang JH, Comstock JC (2014) Species-specific detection and identification of Fusarium species complex, the causal agent of sugarcane pokkah boeng in china. PLoS ONE 9(8):e104195CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Wang ZP, Liu L, Deng YC, Li YJ, Zhang GM, Lin SH, He TG (2017) Establishing a forecast mathematical model of sugarcane yield and Brix reduction based on the extent of pokkah boeng disease. Sugar Technol 19(6):656–661CrossRefGoogle Scholar
  5. 5.
    Wang ZP, Sun HJ, Guo Q, Xu SQ, Wang JH, Lin SH, Zhang MQ (2017) Artificial inoculation method of pokkah boeng disease of sugarcane and screening of resistant germplasm resources in subtropical China. Sugar Technol 19(3):283–292CrossRefGoogle Scholar
  6. 6.
    Zhang CK, Wang JQ, Tao H, Dang X, Wang Y, Chen MP, Zhai ZZ, Yu WY, Xu LP, Bo SW, Lu GD, Wang ZH (2015) FvBck1, a component of cell wall integrity MAP kinase pathway, is required for virulence and oxidative stress response in sugarcane pokkah boeng pathogen. Front Microbiol 6:1096PubMedPubMedCentralGoogle Scholar
  7. 7.
    Lin ZY, Wang JH, Bao YX, Guo Q, Powell CA, Xu SQ, Chen BS, Zhang MQ (2016) Deciphering the transcriptomic response of Fusarium verticillioides in relation to nitrogen availability and the development of sugarcane pokkah boeng disease. Sci Rep 6:29692CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Lin S, Zhou MM, Chen T, Chen GS, Zhou YF, Pan DR (2012) The expression analysis of pathogenesis-related protein encoding genes in chewing cane leaves infected by Gibberella fujikuroi. J Trop Subtrop Bot 20(02):141–148Google Scholar
  9. 9.
    Romão-Dumaresq AS, Dourado MN, de Lima Fávaro LC, Mendes R, Ferreira A, Araújo WL (2016) Diversity of cultivated fungi associated with conventional and transgenic sugarcane and the interaction between Endophytic Trichoderma virens and the host plant. PLoS ONE 11(7):e0158974CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Su YC (2014) Transcriptomics and proteomics of sugarcane response to Sporisorium scitamineum infection and mining of resistance-related genes. Fujian Agriculture & Forestry University, FuzhouGoogle Scholar
  11. 11.
    Zhang XQ (2017) Characteristics of Leifsonia xyli subsp.xyli and influences of its inoculation on physiology and gene expressions in sugarcane. College of Agriculture, Guangxi University, NanningGoogle Scholar
  12. 12.
    Zhang YY (2015) Differentially expressed microRNAs in sugarcane challenged by Sporisorium scitamineum and their target gene function identification. Fujian Agriculture & Forestry University, FuzhouGoogle Scholar
  13. 13.
    Bano A, Muqarab R (2017) Plant defence induced by PGPR against Spodoptera litura in tomato (Solanum lycopersicum L.). Plant Biol 19(3):406CrossRefPubMedGoogle Scholar
  14. 14.
    Benveniste I, Tijet N, Adas F, Philipps G, Salaün JP, Durst F (1998) Cyp86a1 from arabidopsis thaliana encodes a cytochrome p450-dependent fatty acid omega-hydroxylase. Biochem Biophys Res Commun 243(3):688–693CrossRefPubMedGoogle Scholar
  15. 15.
    Höfer R, Briesen I, Beck M, Pinot F, Schreiber L, Franke R (2008) The arabidopsis cytochrome p450 cyp86a1 encodes a fatty acid ω-hydroxylase involved in suberin monomer biosynthesis. J Exp Bot 59(9):2347–2360CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Wellesen K, Durst F, Pinot F, Benveniste I, Nettesheim K, Wisman E et al (2001) Functional analysis of the lacerata gene of arabidopsis provides evidence for different roles of fatty acid ω-hydroxylation in development. Proc Natl Acad Sci USA 98(17):9694–9699CrossRefPubMedGoogle Scholar
  17. 17.
    Xiao F, Goodwin SM, Xiao Y, Sun Z, Baker D, Tang X et al (2004) Arabidopsis cyp86a2 represses pseudomonas syringae type iii genes and is required for cuticle development. EMBO J 23(14):2903–2913CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Benveniste I, Bronner R, Wang Y, Compagnon V, Michler P, Schreiber L et al (2005) Cyp94a1, a plant cytochrome p450-catalyzing fatty acid ω-hydroxylase, is selectively induced by chemical stress in vicia sativa, seedlings. Planta 221(6):881–890CrossRefPubMedGoogle Scholar
  19. 19.
    Dobritsa AA, Shrestha J, Morant M, Pinot F, Matsuno M, Swanson R et al (2009) Cyp704b1 is a long-chain fatty acid omega-hydroxylase essential for sporopollenin synthesis in pollen of arabidopsis. Plant Physiol 151(2):574–589CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Serra O, Soler M, Hohn C, Sauveplane V, Pinot F, Franke R et al (2009) Cyp86a33-targeted gene silencing in potato tuber alters suberin composition, distorts suberin lamellae, and impairs the periderm’s water barrier function. Plant Physiol 149(2):1050–1060CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Compagnon V, Diehl P, Benveniste I, Meyer D, Schaller H, Schreiber L et al (2009) Cyp86b1 is required for very long chain omega-hydroxyacid and alpha, omega-dicarboxylic acid synthesis in root and seed suberin polyester. Plant Physiol 150(4):1831–1843CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Li H, Pinot F, Sauveplane V, Werck-Reichhart D, Diehl P, Schreiber L et al (2010) Cytochrome p450 family member cyp704b2 catalyzes the {omega-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice. Plant Cell 22(1):173CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Wang ZP, Lin SH, Liang Q, Li YJ, Li CN, Duan WX, He TG (2017) Effect of sugarcane canopy structure on pokkah boeng disease resistance. J Chin Agric Univ 22(7):40–46Google Scholar
  24. 24.
    Wang WW, Tang L, Zhou WL, Yang Y, Gao B, Zhao YF, Wang W (2014) Progress in the biosynthesis and metabolism of glutathione. China Biotechnol 34(07):89–95Google Scholar
  25. 25.
    Zhang X, Tao L, Qiao S, Du BH, Guo CH (2017) Roles of glutathione S-transferase in plant tolerance to abiotic stresses. China Biotechnol 37(3):92–98Google Scholar
  26. 26.
    Que YX, Li JW, Zeng JS, Ruan MH, Xu LP, Zhang MQ (2008) Molecular cloning and characterisation of a non-TIR-NBS-LRR type disease resistance gene analogue from sugarcane. Sugar Technol 10(1):71–73CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research InstituteGuangxi Academy of Agricultural Sciences, Sugarcane Research Center, Chinese Academy of Agricultural ScienceNanningChina

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