Analysis of differential gene expression of the transgenic pig with overexpression of PGC1α in muscle

  • Hao Gu
  • Jianan Li
  • Fei Ying
  • Bo Zuo
  • Zaiyan XuEmail author
Original Article


In order to better understand the key regulatory mechanisms of PGC1α in muscle fiber type transition, the RNA-seq was used to compare the change of gene expression in gastrocnemius muscles between wild type pigs and transgenic pigs with overexpression of PGC1α gene in muscle. 371 differentially expressed genes (P ≤ 0.05 and Ratio ≥ 2), including 184 up-regulated genes and 187 down-regulated genes, were identified. Five main signaling pathways including metabolic pathways, ECM-receptor interaction, PPAR signaling pathway, adipocytokine signaling pathway and insulin signaling pathway, were authenticated using KEGG pathway analysis. Our results indicate that the fat metabolism pathway plays an important role in the transformation of muscle fiber types regulated by PGC1α.


RNA-seq Transgenic pig PGC1α 



This work was financially supported by National Key Project for Transgenic Grant Nos. 2018ZX08010-12B and 2016ZX08006-002, The Natural Science Foundation of Hubei Province (Grant No. 2018CFA015) and the Fundamental Research Funds for the Central Universities (Program No. 2662018PY037).

Compliance with ethical standards

Conflict of interest

The authors have declared that no conflict of interest exists.

Supplementary material

11033_2019_4805_MOESM1_ESM.docx (3.4 mb)
Supplementary material 1 (DOCX 3463 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs and Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.The Cooperative Innovation Center for Sustainable Pig ProductionWuhanPeople’s Republic of China

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