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Molecular cloning and expression analysis of SRLK1 gene in self-incompatible Asteraceae species Erigeron breviscapus

  • Wei ZhangEmail author
  • Mo Chen
  • Heng-ling Meng
  • Jin Yang
  • Xiang Wei
  • Sheng-chao YangEmail author
Original Article

Abstract

Based on the transcriptome data, using RACE techniques, we cloned the full-length EbSRLK1 gene in a medicinal, self-incompatible Asteraceae species, Erigeron breviscapus. Bioinformatics approaches were used to analyze the DNA and protein sequences, physical and chemical properties, and domains of the encoded protein. The full-length EbSRLK1 cDNA is 2891 base pairs (bp) with an open reading frame (ORF) of 2634 bp, which encodes the EbSRLK1 protein with 878 amino acids and an estimated molecular weight of 98.13 kD. The EbSRLK1 protein has the characteristic domain structure of S-locus receptor-like protein kinases, which contains one transmembrane domain but lacks the signal peptide. Quantitative real-time PCR (qRT-PCR) analysis showed that the EbSRLK1 gene is lowly expressed in roots, stems and leaves, but highly expressed in flowers, especially in flowers one day prior to opening. Western blot analysis showed that the EbSRLK1 protein is expressed in stems, leaves, and flowers, but is almost undetectable in roots. The EbSRLK1 protein expression is induced in self-pollinated but not in cross-pollinated E. breviscapus flowers. Cloning and expression analysis of EbSRLK1 lay a solid foundation for elucidating the role of EbSRLK1 in regulating self-incompatibility in E. breviscapus.

Keywords

Erigeron breviscapus SRLK gene RACE cloning Gene expression 

Notes

Acknowledgements

This study was funded by National Natural Science Foundation of China (NSFC) (Grant No: 81503184, 81760692), the Project of Young and Middleaged Talent of Yunnan Province (Grant No. 2017HB076). Doctor and Master Specific Projects of Honghe University (XJ17B09).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Honghe UniversityMengziChina
  2. 2.National and Local Joint Engineering Research Center on Germplasm Utilization & Innovation of Chinese Medicinal Materials in SouthwestYunnan Agricultural UniversityKunmingChina

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