Molecular Biology Reports

, Volume 46, Issue 2, pp 2461–2471 | Cite as

H2O2 promotes the aging process of melanogenesis through modulation of MITF and Nrf2

  • Hyeju Ko
  • Moon-Moo KimEmail author
Original Article


The purpose of this study is to investigate the effect of H2O2 on the aging of melanogenesis in human melanocytes. The staining of SA-β-galactosidase, an aging marker, was remarkably increased in the cells aged with H2O2 at 62.5 µM or more compared with young cells. The intracellular H2O2 level and melanin synthesis were also reduced in both H2O2-treated cells and senescent cells compared with young cells in DCFH-DA assay. Both the senescent cells and the H2O2-treated cells showed higher expression level of Catalase than young cells in western blot and immunofluorescence staining. Furthermore, the expression levels of TRP-1, TRP-2 and p300 were reduced in both senescent cells and the H2O2-treated cells, but that of SIRT-1 was inverted compared with young cells. In addition, H2O2 reduced the expression level of MITF but increased that of Nrf2 in nucleus. Those results indicate that the expression levels of antioxidant enzymes in senescent cells and H2O2-treated cell are upregulated, but the expression levels of proteins involved in melanin synthesis are downregulated. Above findings suggest that H2O2 could play a key role in the aging process of melanogenesis through modulation of MITF and Nrf2.


Melanocytes Aging H2O2 MITF SIRT-1 P300 



3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide


2′,7′-Dichlorodihydrofluorescein diacetate


Methionine-S-sulfoxide reductase


Methionine-R-sulfoxide reductase


Superoxide dismutase


Tyrosinase-related protein





This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No.2017R1D1A3B06028000).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Ethical approval and informed consent not required for the paper.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Chemistry & BiologyDong-Eui UniversityBusanRepublic of Korea
  2. 2.Department of Applied ChemistryDong-Eui UniversityBusanRepublic of Korea

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