Molecular Biology Reports

, Volume 46, Issue 2, pp 2523–2528 | Cite as

Vincristine combination with Ca+2 channel blocker increase antitumor effects

  • Ali Taghizadehghalehjoughi
  • Selma SezenEmail author
  • Ahmet Hacimuftuoglu
  • Medine Güllüce
Original Article


In this study, it was aimed to determine the effects of Amlodipine, a calcium channel blocker and vincristine (VCR) an antineoplastic, on human neuroblastomas using different doses. The cytotoxicity assays of the study were performed using the MTT method depending on time and concentration. After obtaining the mixture (up to 85% for SH-SY5Y) and sufficient branches (cortex neurons), the cells were treated with amlodipine (10 µM) and vincristine (0.5, 1 and 2 µg) at different concentrations for 24 h. MTT assay was performed by the commercially available kit (Sigma Aldrich, USA). Cells were harvested, washed and stained with PI and Annexin V, respectively, according to the manufacturer’s protocol (Biovision, USA). Than analyzes were carried out. The results were quite impressive. When amlodipine (10 µM) was administered alone there was little change compared to the control. However, all doses of amlodipine (10 µM) and vincristine (0.5, 1 and 2 µg) were greater than the deaths in the doses alone (0.5, 1 and 2 µg) of vincristine alone. (P < 0.05). As a result, the combination of vincristine and amlodipine is more effective than vincristine alone in reducing the viability of cancer cells.


Neuroblastoma Calcium channel blockers Amlodipine Vincristine 


Compliance with ethical standards

Conflict of interest

There is no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Pharmacology and Toxicology, Faculty of Veterinary MedicineErzurumTurkey
  2. 2.Department of Medical PharmacologyAtatürk UniveristyErzurumTurkey
  3. 3.Department of Biology, Faculty of ScienceAtaturk UniversityErzurumTurkey

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