Molecular Biology Reports

, Volume 46, Issue 2, pp 2505–2512 | Cite as

Serum levels of adiponectin and vitamin D correlate with activity of Rheumatoid Arthritis

  • Sholeh Khajoei
  • Maryam Hassaninevisi
  • Nahid Kianmehr
  • Farhad Seif
  • Majid Khoshmirsafa
  • Mehdi Shekarabi
  • Azam Samei
  • Anousheh HaghighiEmail author
Original Article


Rheumatoid arthritis (RA) is a chronic inflammatory disease in which numerous cells and mediators affect inflammatory conditions and disease severity. To compare the serum levels of adiponectin, vitamin D, copper, and zinc in patients with RA and to investigate the relationship between these parameters and RA severity. Ninety patients with RA and 30 healthy controls participated in this cross-sectional case-control study between November 2016 and April 2017; according to the ACR/EULAR criteria for RA. Serum levels of adiponectin were determined by ELISA; copper and zinc by colorimetric spectrophotometry; and vitamin D by HPLC. Kruskal-Wallis and Spearman tests were performed using SPSS software and data were depicted by GraphPad Prism software. Compared with healthy controls, the serum level of adiponectin was significantly increased, whereas vitamin D was significantly decreased in patients with RA. Adiponectin and vitamin D levels were inversely correlated in RA subgroups (P < 0.001, r = − 0.410). Adiponectin and vitamin D correlated with RA severity. Furthermore, no significant difference was found in copper and zinc levels between RA groups and controls. The definitive roles of adiponectin, vitamin D, copper, and zinc are not completely determined in RA development. Based on disease activity, these parameters can modulate inflammatory conditions, thus they have the potential to be used as promising therapeutic biomarkers to follow up the severity of disease, as well as the progression and treatment success in patients with RA.


Rheumatoid arthritis Adiponectin Vitamin D Copper Zinc 



C-reactive protein


Disease activity score


Erythrocyte sedimentation rate


Enzyme-linked immunosorbent assay


High-performance liquid chromatography




Matrix metalloproteinase


Nuclear factor-kappa B


Rheumatoid arthritis


Systemic lupus erythematosus


Tumor necrosis factor



This study was supported by a Grant of Rasoul Akram hospital. We would also appreciate the kind staff of Massoud clinical laboratory for their collaboration.

Compliance with ethical standards

Conflict of interest

The authors declare no financial or non-financial conflict of interest to in this study.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Sholeh Khajoei
    • 1
  • Maryam Hassaninevisi
    • 1
  • Nahid Kianmehr
    • 1
  • Farhad Seif
    • 2
    • 3
  • Majid Khoshmirsafa
    • 2
    • 4
  • Mehdi Shekarabi
    • 2
    • 4
  • Azam Samei
    • 2
    • 3
  • Anousheh Haghighi
    • 1
    Email author
  1. 1.Department of RheumatologyIran University of Medical SciencesTehranIran
  2. 2.Department of Immunology, School of MedicineIran University of Medical SciencesTehranIran
  3. 3.Neuroscience Research CenterIran University of Medical SciencesTehranIran
  4. 4.Immunology Research Center, Institute of Immunology and Infectious DiseasesIran University of Medical SciencesTehranIran

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