Anti-proliferative and anti-apoptotic potential effects of epigallocatechin-3-gallate and/or metformin on hepatocellular carcinoma cells: in vitro study
- 149 Downloads
The effects of epigallocatechin-3-gallate (EGCG) and metformin single treatment have been tested against hepatocellular carcinoma (HCC). This study aimed to assess the combination effects of EGCG and metformin on proliferation and apoptosis of HepG2cells and identified new potential molecular targets. The effect of EGCG and metformin against cell proliferation in HepG2 was determined using MTT assay. Reverse transcription polymerase chain reaction was applied to examine the gene expression of cyclin D1, lncRNA-AF085935, caspase-3, survivin and VEGF. The level of protein expression of glypican-3 was assessed by western blot. In HepG2 cells, EGCG and metformin combination treatment exhibited high significant effect against tumor proliferation. It significantly reduced cyclin D1, lncRNA-AF085935, glypican-3 and promoted apoptosis through increasing caspase3 and decreasing survivin compared to control cells. Moreover, EGCG and metformin treated cells showed decreased expression levels of VEGF. Our study provided new insights of the anticarcinogenic effects of EGCG and metformin on HCC through their effects on glypican-3 and lncRNA-AF085935.
KeywordsHepatocellular carcinoma EGCG Metformin Glypican-3 LncRNA-AF085935
Compliance with Ethical Standards
Conflict of interest
All authors declared that there was no conflict of interest.
- 18.Li S, Wu L, Jiao F, Li J, Liu T, Rong Z, Shizan X, Keran C, Yuqing Z, Shunfeng Z, Rui K, Kan C, Fan W, Yujing X, Lu J, Yingqun Z, Weiqi D, Chuanyong G (2016) In vitro and in vivo study of epigallocatechin-3-gallate-induced apoptosis in aerobic glycolytic hepatocellular carcinoma cells involving inhibition of phosphofructokinase activity. Sci Rep 6:28479CrossRefPubMedPubMedCentralGoogle Scholar
- 25.Cai X, Hu X, Cai B, Wang Q, Li Y, Tan X, Hu H, Chen X, Huang J, Cheng J, Jing X (2013) Metformin suppresses hepatocellular carcinoma cell growth through induction of cell cycle G1/G0 phase arrest and p21CIP and p27KIP expression and downregulation of cyclin D1 in vitro and in vivo. Oncol Rep 30:2449–2457CrossRefPubMedGoogle Scholar
- 29.Xiao-ting Z, Ji-hang Y, Teng-teng Z, Yang-yang L and Xiao-yang C. (2016) Long noncoding RNA glypican 3 (GPC3) antisense transcript 1 promotes hepatocellular carcinoma progression via epigenetically activating GPC3. FEBS J. 283:3739–3754Google Scholar
- 47.Shirakami Y, Shimizu M, Adachi S (2009) (–)-Epigallocate-chin gallate suppresses the growth of human hepatocellular carcinoma cells by inhibiting activation of the vascular endothelial growth factor-vascular endothelial growth factor receptor axis. Cancer Sci 100:1957–1962. [PubMed: 19558547]CrossRefPubMedGoogle Scholar