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Molecular Biology Reports

, Volume 46, Issue 2, pp 1931–1940 | Cite as

Characterization of aneuploidy in interspecific hybrid between Urochloa ruziziensis (R. Germ. & Evrard) Crins and Urochloa decumbens (Stapf) R. D. Webster

  • Isabella de Campos Moraes
  • Gabriel de Campos Rume
  • Fausto Souza Sobrinho
  • Vânia Helena TechioEmail author
Original Article

Abstract

The aim of the study was to characterize the type of aneuploidy present in the hybrid Urochloa ruziziensis × Urochloa decumbens and to confirm the origin of the additional chromosomes through comparative analysis of the hybrid and parental karyotypes. C and CMA banding techniques were used for chromosome differentiation. The parental genotypes showed 36 chromosomes. The hybrid presented plants with 36 + 2 chromosomes and plants with 36 + 1 chromosomes. Urochloa ruziziensis (4x) presented four chromosomes with CMA and C bands co-located in the terminal position. In U. decumbens, four chromosomes presented terminal CMA bands, eight chromosomes were distinguished by C banding with pericentromeric and terminal bands, one chromosome with terminal band at both ends and one chromosome presented one C terminal band. For the hybrid, CMA bands were found on five chromosomes and C bands on seven chromosomes, all in terminal position. Aneuploidy was identified in pairs 3′ and 4′ in the hybrid plants with 36 + 2 chromosomes, characterizing it as double trisomy. The karyotype of hybrid plants with 36 + 1 chromosomes indicated elimination of the additional chromosome identified in pair 4′ and maintenance of trisomy on pair 3′. The comparative analysis of karyotypes indicates that the additional chromosomes that characterize the trisomy were inherited from U. ruziziensis (artificial tetraploid).

Keywords

Brachiaria Trisomy Karyotype Chromosome banding 

Notes

Acknowledgements

The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq, Fundação de Amparo à pesquisa de Minas Gerais – FAPEMIG e Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES for the financial support to research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biology/DBI-Plant Cytogenetics LaboratoryFederal University of Lavras (UFLA)LavrasBrazil
  2. 2.Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Embrapa Gado de LeiteJuiz de ForaBrazil

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