Molecular Biology Reports

, Volume 46, Issue 2, pp 1855–1871 | Cite as

The effects of Pueraria mirifica extract, diadzein and genistein in testosterone-induced prostate hyperplasia in male Sprague Dawley rats

  • Jamaludin MohamadEmail author
  • Siti Saleha Masrudin
  • Zazali Alias
  • Nur Airina Muhamad
Original Article


Pueraria mirifica (PM) is a medicinal plant native to Thailand contained high amount of phytoestrogen and possesses anticancer activity. This study reports the effect of P. mirifica extract, phytoestrogen of diadzein and genistein for its benign prostate hyperplasia properties in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. The P. mirifica extract was evaluated for its total phenols, flavonoid and antioxidant activity using DPPH, FRAP and metal chelating assay. The assessment of P. mirifica, diadzein and genistein against benign prostate hyperplasia was determined in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. The total phenol was higher than flavonoid but showed low antioxidant activity of DPPH, FRAP and metal chelating. The aqueous PM extract at 1000 mg/kg significantly increased testosterone levels in testosterone-induced rats by 13% while diadzein and genistein increased it by 11% and 17% respectively. However, levels of FSH, LH, triglyceride and HDL are not affected by the oral administration of PM, diadzein and genistein to the rats. Similarly, total protein, albumin, globulin, total bilirubin, conjugated bilirubin, alkaline phosphatase, alanine aminotransferase, AST, and G-glutamyltransferase showed no significant difference as compared with negative control rats. The body weight of the rats, testis, kidney and liver showed no toxic effect. The zinc content increased significantly and the zinc transporter gen of ZnT4 and ZIP4 highly expressed suggesting that the PM, diadzein and genistein plays essential role in modulating prostate zinc homeostasis. Similarly, the expression of IL-6, AR and ER was significantly reduced indicating functioning in regulation of prostate growth and acts as anti-inflammatory role in preventing BPH. In conclusion, the results indicated that PM reduced BPH and contributed to the regulation in the zinc transport expression of the prostate cells in the benign prostate hyperplasia (BPH).


Antioxidant Phytoestrogen Prostate hyperplasia Pueraria mirifica Testosterone Zinc transport 



Pueraria mirifica


Benign prostatic hyperplasia


Prostatic weight


prostatic index


Messenger ribonucleic acid




Ferric ion reducing antioxidant power


Follicle-stimulating hormone


Luteinizing hormone


High density lipid


Aspartate aminotransferase


Zinc transporter ZnT4


Zrt-Irt-like protein


Interleukin 6


Androgen receptor


Estrogen receptor



We are grateful to the Institute of Biological Sciences for providing laboratory facilities to conduct the research works.


This research was supported by the University of Malaya, Grant No. PV010-2012A.

Compliance with ethical standards

Conflict of interest

The author declare that they have no conflict of interest.

Ethical approval

This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of National Institute of Health. The protocol was approved by the University of Malaya Animal Care and Use Committee (No: ISB/30/05/2012/SSM(R)).

Informed consent

Informed consent was obtained from all researches participates in the study.


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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Institute of Biological Sciences, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia
  2. 2.Human Anatomy Department, Faculty of Medicine and Health SciencesUniversity Putra MalaysiaSelangorMalaysia

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