In vitro antioxidant, anti-glycation and immunomodulation activities of fermented blue-green algae Aphanizomenon flos-aquae
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To clarify the antioxidant, anti-glycation and immunomodulatory capacities of fermented blue-green algae Aphanizomenon flos-aquae (AFA), hot aqueous extract suspensions made from 10% AFA were fermented by Lactobacillus plantarum AN7 and Lactococcus lactis subsp. lactis Kushiro-L2 strains isolated from a coastal region of Japan. The DPPH and O2− radical scavenging capacities and Fe-reducing power were increased in the fermented AFA. The increased DPPH radical scavenging capacity of the fermented AFA was fractionated to mainly < 3 kDa and 30–100 kDa. The increased O2− radical scavenging capacities were fractionated to mainly < 3 kDa. Anti-glycation activity in BSA-fructose model rather than BSA-methylglyoxal model was increased by the fermentation. The increased anti-glycation activity was fractionated to mainly 30–100 kDa. The NO concentration in the murine macrophage RAW264.7 culture media was high with the fermented AFA. The increased immunomodulation capacity was also fractionated to mainly 30–100 kDa. These results suggest that the fermented AFA is a more useful material for health foods and supplements.
KeywordsBlue-green algae Aphanizomenon flos-aquae Fermentation Antioxidant Anti-glycation RAW264.7 cells
This work was partially supported by the Japan Health & Research Institute, Tokyo, Japan; Suzuki Nori Co., Choshi, Japan; and Dr’s Choice, Tokyo, Japan.
Compliance with ethical standards
Conflict of interest
The authors do not declare any conflicts of interest.
- 3.Kushak C, Drapeau EM, Van Cott HH (2000) Winter, favorable effects of blue-green algae Aphanizomenon flos-aquae on rat plasma lipids. J Am Nutr Assoc 2:59–65Google Scholar
- 5.Taveme YJ, Merkus D, Bogers AJ, Halliwell B, Duncker DJ, Lyons TW (2018) Reactive oxygen species: Radical factors in the evolution of animal life: a molecular timescale from earth’s earliest history to the rise of complex life. Bioessays 40: https://doi.org/10.1002/bies.201700158
- 8.Birnbaum JH, Wanner D, Gietl AF, Saake A, Kündig TM, Hock C et al (2018) Oxidative stress and altered mitochondrial protein expression in the absence of amyloid-β and tau pathology in iPSC-derived neurons from sporadic Alzheimer’s disease patients. Stem Cell Res 27:121–130CrossRefPubMedGoogle Scholar
- 25.Nuzzo D, Presti G, Picone P, Galizzi G, Gulotta E, Giuliano S et al (2018) Effects of the Aphanizomenon flos-aquae extract (Klamin®) on a neurodegeneration cellular model. Oxid Med Cell Longev. 9089016, https://doi.org/10.1155/2018/9089016
- 27.Mohammed SA, Abdelhafez HM, Eid FA, Abdel-Raouf OM, Ibrahim RM (2016) The possible anti-inflammatory role of the blue green algae, Aphanizomenon flos-aquae on liver of adult male rats. J Biosci Appl Res 2:414–425Google Scholar
- 41.Tallino S, Duffy M, Ralle M, Cortés MP, Latorre M, Burkhead JL (2015) Nutrigenomics analysis reveals that copper deficiency and dietary sucrose up-regulate inflammation, fibrosis and lipogenic pathways in a mature rat model of nonalcoholic fatty liver disease. J Nutr Biochem 26:996–1006CrossRefPubMedPubMedCentralGoogle Scholar
- 56.Mysliwa KB, Solymosi K (2017) Phycobilins and phycobiliproteins used in food industry and medicine. J Mini-Rev Med Chem 17:1173–1193Google Scholar
- 57.Kondo S, Kuda T, Nemoto M, Usami Y, Takahashi H, Kimura B (2016) Protective effects of rice bran fermented by Saccharomyces cerevisiae Misaki-1 and Lactobacillus plantarum Sanriki-SU8 in dextran sodium sulphate-induced inflammatory bowel disease model mice. Food Biosci 16:44–49CrossRefGoogle Scholar