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Molecular Biology Reports

, Volume 46, Issue 2, pp 1661–1666 | Cite as

Serum miR-30c-5p is a potential biomarker for multiple system atrophy

  • Annamaria VallelungaEmail author
  • Tommaso Iannitti
  • Giovanna Dati
  • Sabrina Capece
  • Marco Maugeri
  • Ersilia Tocci
  • Marina Picillo
  • Giampiero Volpe
  • Autilia Cozzolino
  • Massimo Squillante
  • Giulio Cicarelli
  • Paolo Barone
  • Maria Teresa Pellecchia
Original Article
  • 194 Downloads

Abstract

Multiple system atrophy (MSA) is a neurodegenerative disease that belongs to the α synucleinopathies. Clinically, there is an overlap between MSA and Parkinson’s disease (PD), especially at the early disease stage. However, these two pathologies differ in terms of disease progression. Currently, no biomarker exists to differentiate MSA from PD. MicroRNAs are non-coding RNAs implicated in gene expression regulation. MiRNAs modulate cellular activity and they control a range of physiological and pathological functions. miRNAs are found in biofluids, such as blood, serum, plasma, saliva, and cerebrospinal fluid. Many groups, including ours, found that circulating miRNAs are differently expressed in blood, plasma, serum and cerebrospinal fluid of PD and MSA patients. In the present study, our primary aim was to determine if serum mir-30-5p and mir-148b-5p can be used as biomarkers for early diagnosis of PD and/or MSA. Our secondary goal was to determine if serum levels of those miRNAs can be correlated with the patients’ clinical profile. Using quantitative PCR (qPCR), we evaluated expression levels of miR-30c-5p and miR148b-5p in serum samples from PD (n = 56), MSA (n = 49), and healthy control (n = 50) subjects. We have found that miR-30c-5p is significantly upregulated in MSA if compared with PD and healthy control subjects. Moreover, serum miR-30c-5p levels correlate with disease duration in both MSA and PD. No significant difference was found in miR-148b-5p among MSA, PD and healthy control subjects. Our results suggest a possible role of serum miR-30-5p as a biomarker for diagnosis and progression of MSA.

Keywords

Parkinson’s disease MiRNAs Multiple system atrophy MiR-30c-5p Biomarker Synucleinopathies 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no known conflicts of interest in relation to this article.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Annamaria Vallelunga
    • 1
    Email author
  • Tommaso Iannitti
    • 2
  • Giovanna Dati
    • 1
  • Sabrina Capece
    • 1
  • Marco Maugeri
    • 3
  • Ersilia Tocci
    • 4
  • Marina Picillo
    • 1
  • Giampiero Volpe
    • 5
  • Autilia Cozzolino
    • 6
  • Massimo Squillante
    • 5
  • Giulio Cicarelli
    • 6
  • Paolo Barone
    • 1
  • Maria Teresa Pellecchia
    • 1
  1. 1.Neuroscience Section, Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”University of SalernoSalernoItaly
  2. 2.KWS BioTestPortisheadUK
  3. 3.Department of Rheumatology and Inflammation Research, Sahlgrenska AcademyUniversity of GothenburgGothenburgSweden
  4. 4.Laboratorio Analisi PO Serra San BrunoASP Vibo ValentiaVibo ValentiaItaly
  5. 5.Clinica NeurologicaAOU San Giovanni di Dio e Ruggi d’AragonaSalernoItaly
  6. 6.AO San Giuseppe MoscatiAvellinoItaly

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