Polymorphism analyses of 19 STRs in Labrador Retriever population from China and its heterozygosity comparisons with other retriever breeds
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Pure breed dogs of Western origin are increasingly more popular in China as is a need to differentiate breeds and individual dogs for personal and forensic reasons. Research on genetic diversities of the canine population in China is rarely conducted. In this study, genetic distributions and forensic efficiencies of 19 canine STR loci in Labrador Retriever population from China were evaluated by using one available commercial canine kit in China. This panel was used to genetically define 214 Labrador Retrievers in China, as an example of one of the most important Western breeds and to compare them with Labrador Retrievers from America based on three overlapping STR loci. Moreover, genetic relationship analyses between Labrador Retriever population and two reference populations in America were performed. All 19 STR loci were polymorphic and conformed to Hardy–Weinberg equilibrium in the studied population. The STR panel was able to discern individual dogs with a high degree of accuracy. Breed-wide genetic heterozygosity comparisons based on present and published allele frequencies revealed that the studied population had the lower genetic heterozygosity than canine populations in America. Principal component analysis among Labrador Retriever population and other reference populations showed that the studied Labrador Retrievers were genetically close to the retriever breeds in America. Population genetic structure analyses among these canine breeds further revealed genetic differentiations between the studied Labrador Retriever population and other compared breeds. In conclusion, these STR loci had relatively high forensic values in Labrador Retriever population in China, which could be employed for individual identification and kinship testing.
KeywordsSTRs Labrador Retriever Polymorphic Forensic values Genetic heterozygosity
This work was supported by Shaanxi Science and Technology Co-ordination and Innovation Project (Grant No. 2015KTCL03-03).
Compliance with ethical standards
Conflict of interest
These authors declared no conflict of interest.
- 2.Halverson JL, Basten C (2005) Forensic DNA identification of animal-derived trace evidence: tools for linking victims and suspects. Croat Med J 46:598–605Google Scholar
- 3.Wang HS, Wang X, Zhang WP (2003) A study of genetic polymorphisms on10STR loci of the police dogs in Hubei area. Forensic Sci Technol 2:005Google Scholar
- 4.Xiong X, Zhang XF, Mu LW, Wang ML, Yang JL, Zhang B, Mang JW, Deng YJ (2013) Genetic polymorphisms of 16 STR loci in Tibetan Mastiff. Fa Yi Xue Za Zhi 29:282Google Scholar
- 6.Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic-mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49:746–756Google Scholar
- 13.Walsh PS, Metzger DA, Higuchi R (1991) Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513Google Scholar
- 16.Kanthaswamy S, Tom BK, Mattila AM, Johnston E, Dayton M, Kinaga J, Erickson BJ, Halverson J, Fantin D, DeNise S, Kou A, Malladi V, Satkoski J, Budowle B, Smith DG, Koskinen MT (2009) Canine population data generated from a multiplex STR kit for use in forensic casework. J Forensic Sci 54:829–840CrossRefGoogle Scholar
- 18.Tereba A (1999) Tools for the analysis of population statistics. Profiles DNA 2:14–16Google Scholar
- 21.Falush D, Stephens M, Pritchard JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567–1587Google Scholar
- 22.Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New YorkGoogle Scholar
- 23.Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic-linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331Google Scholar