Molecular Biology Reports

, Volume 46, Issue 1, pp 1099–1105 | Cite as

CD147 as an apoptosis regulator in spermatogenesis: deciphering its association with matrix metalloproteinases’ pathway

  • Rezvan Asgari
  • Kamran Mansouri
  • Mitra BakhtiariEmail author
  • Asad Vaisi-Raygani
Original Article


CD147 plays an important role in germ cells migration and survival/apoptosis during the spermatogenesis process. However, to best of our knowledge, there is no report on the exact role of CD147 gene in the regulation of germ cells apoptosis through matrix metalloproteinases (MMPs). So, the current study aims to evaluate the role of CD147 gene expression in the regulation of germ cells apoptosis in conjunction with MMPs. Real-Time PCR was applied to investigate the expression of CD147, MMP2, MMP7, and MMP9 genes in the azoospermic patients and fertile males. Receiver-operating characteristic curve was used to interpret gene expression data. According to our results, a significant decrease in the expression of CD147 gene and an increase in MMPs genes expression were observed in infertile patients compared to fertile males. These results proved this fact that the CD147 gene has an important role in the regulation of germ cells apoptosis via a MMPs-dependent pathway.


CD147 MMPs Apoptosis Spermatogenesis Infertility 



Matrix metalloproteinases


Extracellular matrix


Receiver-operating characteristic


Base pair



We would like to thank Dr. Hossein Abdi, and Farzaneh Esmailli for their excellent technical help.


This study has been supported financially by a Grant (96689) from the Vice Chancellor for Research of Kermanshah University of Medical Sciences in Iran.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest to disclose and the manuscript was not published previously. All authors have read the manuscript and approved the final version.

Informed consent

The present study has been approved by the Ethics Committee of the Kermanshah University of Medical Sciences. A signed consent form was obtained from all participants who donated testis tissue samples.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Rezvan Asgari
    • 1
  • Kamran Mansouri
    • 1
  • Mitra Bakhtiari
    • 2
    • 3
    Email author
  • Asad Vaisi-Raygani
    • 4
  1. 1.Medical Biology Research Center (MBRC)Kermanshah University of Medical SciencesKermanshahIran
  2. 2.Department of Anatomical Sciences & Cell BiologyKermanshah University of Medical SciencesKermanshahIran
  3. 3.Fertility and Sterility Research CenterKermanshah University of Medical SciencesKermanshahIran
  4. 4.Department of Clinical BiochemistryKermanshah University of Medical SciencesKermanshahIran

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