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Differential expression of miRNAs related to angiogenesis and adipogenesis in subcutaneous fat of obese and nonobese women

  • Aline S. GasparottoEmail author
  • Diego O. Borges
  • Marina G. M. Sassi
  • Adriana Milani
  • Darwin L. Rech
  • Marcia Terres
  • Pedro B. Ely
  • Mauricio J. Ramos
  • Nelson G. Meihnardt
  • Vanessa S. Mattevi
Original Article
  • 42 Downloads

Abstract

To disclose the mechanisms surrounding obesity, we selected microRNAs (miRNAs) that target genes involved in adipogenesis, angiogenesis, and inflammation and compared their expression levels in the subcutaneous adipose tissue of 40 obese and nonobese women. Mature miRNAs were extracted from subcutaneous adipose tissue samples that were collected during surgery and quantified by real-time polymerase chain reaction. miR-16 was overexpressed in the nonobese group (n-expression ratio = − 151.1; P < 0.001). Furthermore, the expression levels of two other miRNAs were significantly correlated with waist circumference in nonobese women (miR-27b, r = 0.453; P = 0.027 and miR-424-5p, r = 0.502, P = 0.014). Central and total subcutaneous adipose tissue thicknesses were correlated with miR-424-5p levels (r = 0.506, P = 0.034 and r = 0.475, P = 0.046, respectively) in the nonobese group. In the obese group, miR-424-5p expression was correlated with body mass index (r = 0.582, P = 0.018). miR-16 and miR-424 have shown correlations with body-fat-mass-related parameters. Because these miRNAs have vascular endothelial growth factor (VEGF) and its receptors as target genes, they may be involved in the alterations of angiogenesis observed in obesity. In addition, higher levels of miR-27 and miR-424 were correlated with higher fat depot measurements in nonobese women. These results highlight the importance of miRNA expression in subcutaneous adipose tissue and encourage further investigation of miRNAs as prognostic markers.

Keywords

Adipose tissue Obesity microRNA miR-16 miR-424 miR-27 

Notes

Funding

This work has been financially supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil - 471437/2012-3) and the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Brazil - 12/2226-9 and 1153-2551/13-9). A.S.G. and D.O.B. received scholarships from the REUNI program from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study complied with the ethical principles outlined in the Declaration of Helsinki. All enrolled participants were informed and signed informed consent at the beginning of their participation in the study.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Aline S. Gasparotto
    • 1
    Email author return OK on get
  • Diego O. Borges
    • 1
  • Marina G. M. Sassi
    • 1
  • Adriana Milani
    • 2
  • Darwin L. Rech
    • 2
  • Marcia Terres
    • 2
  • Pedro B. Ely
    • 2
  • Mauricio J. Ramos
    • 3
  • Nelson G. Meihnardt
    • 3
  • Vanessa S. Mattevi
    • 1
  1. 1.Programa de Pós-graduação em Ciências da SaúdeUniversidade Federal de Ciências da Saúde de Porto AlegrePorto AlegreBrazil
  2. 2.Irmandade Santa Casa de Misericórdia de Porto AlegrePorto AlegreBrazil
  3. 3.Hospital Nossa Senhora da ConceiçãoPorto AlegreBrazil

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