Advertisement

Molecular Biology Reports

, Volume 46, Issue 1, pp 841–845 | Cite as

MnSOD, CAT and GPx-3 genetic polymorphisms in coronary artery disease

  • Ayşe DoğanEmail author
  • Yusuf Özşensoy
  • Fevzi Sarper Türker
Original Article
  • 49 Downloads

Abstract

In this study, we aimed to determine the gene polymorphisms of antioxidant enzymes that determine or affect antioxidant activity in the occurrence of the disease and/or complications during and after the surgery in patients who were decided to undergo coronary artery bypass surgery due to coronary artery disease. Blood samples taken before operation in 26 coronary artery patients who were decided to be operated according to the international procedure and the phenol/chloroform method was used to isolate DNA. DNA samples were amplified by using polymerase chain reaction (PCR) method with specific primers for MnSOD, CAT, GPx-3 antioxidant gene regions. As a result of the increasing process, the PCR products for the purpose of determining gene polymorphism, NGOMIV SMA f and BSA I restriction enzymes were used for MNSOD, CAT and GPx-3 gene region, respectively. Allele frequencies were determined and compared by Chi square test. VV (46.15%) and VA (53.85%) genotype for MnSOD region, i TT (22.22%), TC (16.67%) and CC (61.11%) genotype for CAT region, and CC (12.50%), TC (25%) and TT (62.50%) genotypes for GPx-3 region were obtained. While there was no statistically significant significance in terms of genotypes obtained in MnSOD and GPx-3 gene regions (P > 0.05), a significant difference was found in the CAT gene region in terms of genotypes (P < 0.01). Although oxidative stress is important in relation to cardiovascular diseases and postoperative complications, virtually no study of antioxidant enzymes in gene polymorphism are included in the literature. Work is lacking in relation to the subject.

Keywords

MnSOD GPx-3 CAT Polymorphism Coronary artery 

Notes

Acknowledgements

This study was funded by Cumhuriyet Üniversitesi (Grant no 1) and Bitlis Eren Üniversitesi (Grant no 2).

References

  1. 1.
    Grundy SM, Pasternak R, Greenland P, Smith S Jr, Fuster V (1999) Assesment of cardiovascular risk by use of multiple risk factor assessment equation. A statement for healthcare professionals from the American Heart Association and the American College of cardiology. Circulation 100:1481–1492CrossRefGoogle Scholar
  2. 2.
    Harrison D, Griendling KG, Landmesser U, Hornig B, Drexlar H (2003) Role of oxidative stress in atherosclerosis. Am J Cardio 91:7–11CrossRefGoogle Scholar
  3. 3.
    Blankenberg S, Rupprechi MJ, Bickel C, Torzewski M, Hafner G, Tiret L et al (2003) Glutathione peroxidase 1 activity and cardiovascular events in patients with coronary artery disease. N Engl J Med 349(17):1605–1613CrossRefGoogle Scholar
  4. 4.
    Cohn LH (2003) Fifty years of open heart surgery. Circulation 107:2168–2170CrossRefGoogle Scholar
  5. 5.
    Lazar HL (2014) Should off-pump coronary artery bypass surgery be abandoned: a potential solution. J Thorac Cardiovasc Surg 148(6):2475–2476CrossRefGoogle Scholar
  6. 6.
    Barzegar AOM, Sciesser CH, Taylor MK (2014) New reagents for detecting free radicals and oxidative stress. Org Blomol Chem 12(35):6757–6766CrossRefGoogle Scholar
  7. 7.
    Gupta RK, Patel AK, Shah N, Chaudhary AK, Jha UK, Yadav UC, Gupta PK, Pakuval U (2014) Oxidative stress and antioxidants in disease and cancer: a review. Asian Pac Cancer Prev 15(11):4405–4409CrossRefGoogle Scholar
  8. 8.
    Ray PD, Huang BW, Tsuji Y (2012) Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal 24(5):981–990CrossRefGoogle Scholar
  9. 9.
    Trachootham D, Alexandre J, Huang P (2009) Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat Rev Drug Discov 8:579–591CrossRefGoogle Scholar
  10. 10.
    Andersen JK (2004) Oxidative stress in neurodegeneration: cause or consequence? Nat Med 10:18–25CrossRefGoogle Scholar
  11. 11.
    Shukla V, Mishra SK, Pant HC (2011) Oxidative stress in neurodegeneration. Adv Pharmacol Scl 572–634Google Scholar
  12. 12.
    Paravicini TM, Touyz RM (2006) Redox signaling in hypertension. Cardiovasc Res 247–258Google Scholar
  13. 13.
    Haigis MC, Yankner BA (2010) The aging stress response. Mol Cell 40(2):333–344CrossRefGoogle Scholar
  14. 14.
    Ishikawa K, Takenaga K, Akimoto M, Koshikawa N, Yamaguchi A, Imanishi H et al (2008) ROS-generating mitochondrial DNA mutations can regulate tumor cell metastasis. Science 320: 661–664CrossRefGoogle Scholar
  15. 15.
    Pearson TA, Mensah GA, Alexander RW et al (2003) Markers of inflammation and cardiovascular disease: application to clinical and public health practice: a statement for healthcare professionals from the centers for disease control and prevention ant the American Heart Association. Circulation 107(3):499–511CrossRefGoogle Scholar
  16. 16.
    Doğan A, Türker FS (2017) Cardiopulmonary bypass and oxidative stress. Chem Res J 2(6):156–162Google Scholar
  17. 17.
    Batinic-Haberle I, Reboucas JS, Spasojevich I (2010) Superoxide dismutase mimics: chemistry, pharmacology and therapeutic potential. Antioxid Redox Signal 13:877–918CrossRefGoogle Scholar
  18. 18.
    Ambrosone CB, Freudenheim JL, Thompson PA, Browman E, Vena JE, Marshall JR, Graham S, Laughlin R, Nemoto T, Shields PG (1999) Manganase superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, risk of breast cancer. Cancer Res 59:602–606Google Scholar
  19. 19.
    Zhou XF, Cui J, De S Al, Chazaro I, Farrer LA, Manolis AJ, Gavras H, Baldwin CT (2005) Polymorphisms in the promoter region of catalase gene and essential hypertension. Dis Markers 21:3–7CrossRefGoogle Scholar
  20. 20.
    Shuvalova YA, Kaminnyi AI, Meshkov AN, Shirokov RO, Samko AN (2012) Association between polymorphisms of eNOS and GPx-1 genes activity of free-radical processes an in-stent restenosis. Mol Cell Biochem 370:241–249CrossRefGoogle Scholar
  21. 21.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Pres, Cold Spring Harbor. pp 9.16–9.19Google Scholar
  22. 22.
    Forsberg L, Lyrenas L, Faire UD, Morgenstern R (2001) A common functional C–T substitution polymorphism in the promoter region of the human catalase gene influences transcription factor binding, reporter gene transcription and is correlated to blood catalase levels. Free Radical Biol Med 30(5):500–505CrossRefGoogle Scholar
  23. 23.
    Voetsch B, Jin RC, Bierl C, Benke KS, Kenet G, Simioni P, Ottaviano F, Damasceno BP, Annichino-Bizacchi JM, Handy DE, Loscalzo J (2007) Promoter polymorphisms in the plasma glutathione peroxidase (GPx-3) gene: a novel risk factor for arterial ischemic stroke among young adults and children. Stroke 38(1):41–49CrossRefGoogle Scholar
  24. 24.
    Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) Touchdown’ PCR to circumvent spurious priming during gene amplification. Nucl Acids Res 19:4008CrossRefGoogle Scholar
  25. 25.
    Ohata T, Mitsuno M, Yamamura M, Tanaka H, Kobayashi Y, Ryomoto M, Yoshioka Y, Miyomoto Y (2007) Minimal cardiopulmonary bypass attenuates neutrophil activation and cytokine release in coronary artery bypass grafting. J Artif Organs 10:92–95CrossRefGoogle Scholar
  26. 26.
    Karahalil B, Kesimci E, Emerce E, Gumus T, Kanbak O (2011) The impact of OGG1, MTH1 and MnSOD gene polymorphisms on 8-hydroxy-2′-deoxyguanosine and cellular superoxide dismutase activity in myocardial ischemia-reperfusion. Mol Biol Rep 38:2427–2435CrossRefGoogle Scholar
  27. 27.
    Neomoto M, Nishimura R, Sasaki T, Hiki Y, Miyashita Y, Nishioka M, Fujimoto K, Sakuma T, Ohashi T, Fukuda K, Eto Y, Tajima N (2007) Genetic association of glutathione peroxidase-1 with coronary artery calcification in type 2 diabetes: a case control study with multi-slice computed tomography. Cardiovasc Diabetol 6:23–27CrossRefGoogle Scholar
  28. 28.
    Wickremasinghe D, Peiris H, Chandrasena LG, Senaratne V, Perera R (2016) Case control feasibility study assessing the association between severity of coronary artery disease with Glutathione Peroxidase 1 (GPX-1) and GPX-1 polymorphism. BMC Cardiovasc Disord 16:111CrossRefGoogle Scholar
  29. 29.
    Türker FS, Doğan A, Ozan G, Kıbar K, Erışır M (2016) Change in free radical and antioxidant enzyme levels in patients undergoing open heart surgery with cardiopulmonary bypass. Oxid Med Cell Longev 2016:1783728CrossRefGoogle Scholar
  30. 30.
    Venardos KM, Perkins A, Headrick J, Kaye DM (2007) Myocardial ischemia-reperfusion injury, antioxidant enzyme systems, and selenium: a review. Curr Med Chem 14:1539–1549CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Ayşe Doğan
    • 1
    Email author
  • Yusuf Özşensoy
    • 2
  • Fevzi Sarper Türker
    • 3
  1. 1.Department of Physiotherapy and Rehabilitation, Health High SchoolBitlis Eren UniversityBitlisTurkey
  2. 2.Department of Veterinary Biometrics and Genetics, Faculty of Veterinary MedicineSivas Cumhuriyet UniversitySivasTurkey
  3. 3.Elazığ Training and Research Hospital Cardiovascular SurgeryUniversity of Health SciencesElazığTurkey

Personalised recommendations