Isolation and characterization of three skeletal troponin genes and association with growth-related traits in Exopalaemon carinicauda
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Growth is among the most important traits for animal breeding. Muscle growth is controlled by different cellular and molecular pathways and environments, and it also relies heavily on high-quality muscle contractions. The troponin complex, composed of troponin T (TnT), troponin C (TnC) and troponin I (TnI), plays a vital role in the regulation of muscle contraction. In this study, the cDNA of EcTnT, EcTnC and EcTnI of the ridgetail white prawn Exopalaemon carinicauda were cloned and characterized. The full length cDNA of EcTnT, EcTnC and EcTnI were 1 373 bp, 692 bp, and 1 475 bp, encoding a protein of 385, 150 and 193 amino acid residues, respectively. The expression of all genes was predominantly detected in abdominal muscle, while extremely lesser expressed in gill and hepatopancreas. Higher expression level of EcTnI was observed in heavier shrimp of the same age during different developmental stages, excepted for 120 days. Eleven single nucleotide polymorphisms (SNPs) were revealed in the three skeletal troponin genes, and only c.TnI66 A>G from EcTnI was significantly associated with both body weight and body length (P < 0.05). In summary, the result of this study suggested that EcTnI is growth-related gene of the troponin complex gene and the presence of SNP suggests that it could be a candidate gene for shrimp genetic improvement research.
KeywordsExopalaemon carinicauda Troponin Gene expression Growth-related SNP
This project was financially supported by the earmarked fund for National Key R & D Program of China (2018YFD0901302), Modern Agro-industry Technology Research System (No. CARS-48), The Program of Shandong Leading Talent (No. LNJY2015002), National Natural Science Foundation of China (No. 31472275) and Qingdao Industrial Development Program Science and Technology Benefit Special Project (17-3-3-62-nsh).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The collection and handling of the animals in this study was approved by the Animal Care and Use Committee at the Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, and all experimental animal protocols were carried out in accordance with national and institutional guidelines for the care and use of laboratory animals at the Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences.
- 4.Chen H, Wang D, Xu G, Zheng C (2012) Cloning, structural analysis and expression of cardiac troponin C (TNNC1) gene in goat. Afr J Biotechnol 11(16):3903–3910Google Scholar
- 5.Jensen D, Reynolds N, Yang YP, Shakya S, Wang ZQ, Stuehr DJ, Wei CC (2015) The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca2+-induced hydrophobicity and alter the interaction with Orai1: a spectroscopic, thermodynamic and kinetic study. BMC Biochem 16(1):1–18CrossRefGoogle Scholar
- 18.Divet A, Paesante S, Grasso C, Cavagna D, Tiveron C, Paolini C, Protasi F, Huchet-Cadiou C, Treves S, Zorzato F (2007) Increased Ca2+ storage capacity of the skeletal muscle sarcoplasmic reticulum of transgenic mice over-expressing membrane bound calcium binding protein junctate. J Cell Physiol 213(2):464–474PubMedCrossRefGoogle Scholar
- 25.Blanck DV, Penteado MDA, Valenti WC, Freitas PDD, Luis J, Da L, Rocha C, Pedro, Galetti M (2016) SNPs within HSC70 gene are associated to growth traits in the Amazon River prawn. Pan-American J Aquat Sci 11(3):210–219Google Scholar
- 33.Zhao YZ, Chen XL, Yang CL, Peng M, Ping-Ping HE, Chen XH (2013) Sequence analysis of four different spliced variants of troponin I gene in Litopenaeus vannamei. J Shanghai Ocean Univ 22(6):807–814Google Scholar
- 41.Marden JH, Fitzhugh GH, Girgenrath M, Wolf MR, Girgenrath S (2001) Alternative splicing, muscle contraction and intraspecific variation: associations between troponin T transcripts, Ca(2+) sensitivity and the force and power output of dragonfly flight muscles during oscillatory contraction. J Exp Biol 204(20):3457–3470PubMedGoogle Scholar
- 46.Zhao Y, Yao Q, Tang X, Wang Q, Yin H, Hu Z, Lu J, Chen K (2007) Cloning and homologic analysis of Tpn I gene in silkworm Bombyx mori. Afr J Biotechnol 6(6):672–676Google Scholar
- 47.Umasuthan N, Elvitigala DAS, Revathy KS, Lee Y, Whang I, Park MA, Lee J (2013) Identification and in silico analysis of a novel troponin C like gene from Ruditapes philippinarum (Bivalvia: Veneridae) and its transcriptional response for calcium challenge. Gene 519(1):194–201PubMedCrossRefGoogle Scholar
- 49.Ma T, Zhou YZ, Xiang FY, Shen J, Zhou JL (2008) Molecular cloning of troponin I gene from the hard tick Rhipicephalus haemaphysaloides and its distribution in it. Chin J Vet Sci 28(2):160–162Google Scholar
- 51.Liang XQ, Li YJ, Zhou ZM (1988) The larval development of Exopalaemon carinicauda. J Fish China 12(2):157–168Google Scholar