Molecular Biology Reports

, Volume 46, Issue 1, pp 569–580 | Cite as

Cloning, characterization and paper pulp applications of a newly isolated DyP type peroxidase from Rhodococcus sp. T1

  • Miray Sahinkaya
  • Dilsat Nigar Colak
  • Aysegul Ozer
  • Sabriye Canakci
  • Ilhan Deniz
  • Ali Osman BelduzEmail author
Original Article


A newly identified ligninolytic Rhodococcus strain (Rhodococcus sp. T1) was isolated from forestry wastes (Trabzon/Turkey). The DyP type peroxidase of Rhodococcus sp. T1 (DyPT1) was cloned, characterized and paper treated for industrial applications. Molecular weight of the protein was about 38 kDa. The kinetic parameters were 0.94 mM and 1417.53 µmol/min/mg for Km and Vmax, respectively. The enzyme was active at the temperature range of 25–65 °C and optimum temperature was 35 °C, enzyme was stable up to 6 days at room temperature. Optimum pH of the DyPT1 was 4.0 and it was stable between pH 4.0–6.0 up to 8 days at room temperature. Effects of some metal ions, Hemin, and some chemical agents on DyPT1 were determined. Hemin has implemented protective effects on the stability and the activity of the enzyme in long time periods when added into growing medium. DyPT1 was applied to eucalyptus kraft pulp for analyzing the bleaching efficiency, physical and optical tests of the manufuctared paper were carried out. Application of lignin peroxidase to kraft pulp caused a decrease of 5.2 units for kappa number and an increase from 52.05 to 64.18% in the delignification rate.


Pulp bleaching Lignin peroxidase Rhodococcus sp. Kappa number 



This study was financially supported by Karadeniz Technical University Research Foundation (Project No: FBA-2015-5182).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Miray Sahinkaya
    • 1
  • Dilsat Nigar Colak
    • 2
  • Aysegul Ozer
    • 1
  • Sabriye Canakci
    • 1
  • Ilhan Deniz
    • 3
  • Ali Osman Belduz
    • 1
    Email author
  1. 1.Department of Biology, Faculty of SciencesKaradeniz Technical UniversityTrabzonTurkey
  2. 2.Department of Forestry, Vocational School of DereliGiresun UniversityGiresunTurkey
  3. 3.Department of Forest Industrial Engineering, Faculty of ForestryKaradeniz Technical UniversityTrabzonTurkey

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