Production of eicosapentaenoic acid (EPA, 20:5n-3) in transgenic peanut (Arachis hypogaea L.) through the alternative Δ8-desaturase pathway
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An important alternative source of fish oil is its production by plants through metabolic engineering. To produce eicosapentaenoic acid (EPA, 20:5n-3) in peanut through the alternative Δ8-pathway, a plant expression vector containing five heterologous genes driven by the constitutive 35S promoter respectively, namely, ∆9-elongase (Isochrysis galbana), ∆8-desaturase (Euglena gracilis), ∆5-desaturase (Mortierella alpina), ∆15-desaturase (Arabidopsis thaliana) and ∆17-desaturase (Phytophthora infestans) were transferred into peanut through Agrobacterium-mediated transformation method. The gas chromatography results indicated that the average content of EPA in the leaves of the transgenic lines was 0.68%, and the highest accumulation of EPA in an individual line reached 0.84%. This finding indicates that it is feasible to synthesize EPA in peanut through metabolic engineering and lays the foundations for the production of very-long-chain polyunsaturated fatty acids (VLCPUFAs) in peanut seeds.
KeywordsEicosapentaenoic acid Peanut Δ8-pathway Transgenic plants
Very-long-chain polyunsaturated fatty acids
Multiple cloning site
Cetyltrimethyl ammonium bromide
Fatty acid methyl esters
Gas chromatograph–mass spectrometer
This study was funded by the Shandong Province Peanut Seed Industry Project, the National Natural Science Foundation of China (31601336, 31271757) and the National Opening Project of State Key Laboratory of Crop Biology (2016KF09). The sequencing and assembly were performed by the Beijing Genomics Institute (BGI).
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest.
- 6.Pawlosky RJ, Hibbeln JR, Novotny JA, Salem N (2001) Physiological compartmental analysis of α-linolenic acid metabolism in adult humans. J Lipid Res 42:1257–1265Google Scholar
- 10.Kinney A, Cahoon E, Damude H, Hitz W, Liu ZB, Kolar C (2004) Production of very long chain polyunsaturated fatty acids in oilseed plants, Google Patents 2004Google Scholar
- 11.Kinney A, Cahoon E, Damude H, Hitz W, Liu ZB, Kolar C (2011) Production of very long chain polyunsaturated fatty acids in oil seed plants, Google Patents 2011Google Scholar
- 24.Bhatnagar-Mathur P, Devi MJ, Reddy DS, Lavanya M, Vadez V, Serraj R, Yamaguchi-Shinozaki K, Sharma KK (2007) Stress-inducible expression of At DREB1A in transgenic peanut (Arachis hypogaea L.) increases transpiration efficiency under water-limiting conditions. Plant Cell Rep 26:2071–2082CrossRefGoogle Scholar
- 30.Zhu Q, Yu S, Zeng D, Liu H, Wang H, Yang Z, Xie X, Shen R, Tan J, Li H, Zhao X, Zhang Q, Chen Y, Guo J, Chen L, Liu YG (2017) Development of ‘‘Purple Endosperm Rice’’ by engineering anthocyanin biosynthesis in the endosperm with a high-efficiency transgene stacking system. Mol Plant 10:918–929CrossRefGoogle Scholar