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Molecular Biology Reports

, Volume 45, Issue 6, pp 2555–2561 | Cite as

Cell line cytotoxicity, antiadipogenic and glucose uptake activity of Sarcostemma brevistigma Wight. & Arn.

  • Krishnamoorthy Vijayalakshmi
  • Chinnadurai Immanuel Selvaraj
Original Article
  • 40 Downloads

Abstract

Nature has gifted us with abundant plants possessing medicinal virtues which can cure several illnesses. Currently, diabetes mellitus is a severe threat to human well-being across the world due to the rapidly increasing incidence of diabetes. New effective bioactive drugs are in need, as plants do harbour and are proven to have potential antidiabetic activity than the present hypoglycemic medicines used in clinical therapy. In this study, in vitro cytotoxicity, glucose uptake, and anti-adipogenic activities of the plant extract (methanolic extract) of S. brevistigma were examined using 3T3L1 cell lines. The studies interpreted by MTT cytotoxicity assay and glucose uptake assay by using 3T3-L1 cell lines, it was found that a very low dosage (1 ng/mL) of plant extract showed lesser cytotoxicity effect (1.42%) and considerably higher glucose uptake activity of 38.04% which is equivalent to the glucose uptake shown by 100 nm insulin (40.10%). Though plant extract has antidiabetic activity, it is important to study whether it has any other related side effects when used at higher concentrations. Therefore, in this study, the appropriate non-toxic concentration was optimized.

Keywords

Anti-adipogenic activity Glucose uptake Hypoglycemic agents 3T3L1 S. brevistigma Cytotoxicity 

Notes

Acknowledgements

The author would like to thank VIT, Vellore and ARMATS Biotek, Chennai for providing lab and instrumentation facilities to complete this research work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval and Informed consent

The authors declare that ethical approval and the informed statement is not necessary for this research study.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Biotechnology, School of Biosciences and TechnologyVAIAL, VITVelloreIndia

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