Molecular Biology Reports

, Volume 45, Issue 6, pp 2403–2409 | Cite as

TCF4 and GRM8 gene polymorphisms and risk of schizophrenia in an Iranian population: a case-control study

  • Javad Tavakkoly-Bazzaz
  • Asaad Azarnezhad
  • Negar Mousavi
  • Pouya Salehipour
  • Esmaeil Shahsavand Ananloo
  • Fatemeh AlizadehEmail author
Original Article


TCF4 and GRM8, two significant genes involved in the normal nervous development and glutamate pathway, are thought to be involved in the pathogenesis of schizophrenia (SCZ). We aimed to explore the association of TCF4 and GRM8 gene polymorphisms with risk of SCZ. The rs8766 in TCF4 and rs712723 in GRM8 were selected for genotyping in a set of Iranian case-control samples including 215 patients and 220 matched healthy controls using polymerase chain reaction-restriction fragment length polymorphism. Although rs8766 increased the OR, we found that rs8766 allele and genotype frequencies were not significantly different between case and control groups and a significant association cannot be suggested for the selected SNP. However, allele C and genotype CC (allele C: OR 1.48, 95% CI 1.13–1.94; genotype CC: OR 1.71, 95% CI 1.09–2.68) of rs712723 polymorphism was found to have a significant association with risk of SCZ. Frequency of allele C (P = 0.003) and genotype CC (P = 0.017) was higher in the schizophrenic patients, while allele T (P = 0.003) and genotype TT (P = 0.028) frequencies were found lower in patients. Our findings indicate that rs712723 in GRM8 may play an important role in the pathogenesis of SCZ. However, our conclusion needs to be confirmed in other population.


Schizophrenia Association study TCF4 GRM8 



This research was supported by Tehran University of Medical Sciences under registered Grant 32178. We thank patients and healthy subjects who provided us with a valuable resource of information to greatly assist the research.

Compliance with ethical standards

Conflict of interest

All the authors declare no conflict of interest relevant to this article.

Ethical approval

This study was approved by the Ethics Committee of Tehran University of Medical Sciences, Iran.

Informed consent

Informed consent forms were obtained from all individual participants included in the study.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Medical Genetics, School of MedicineTehran University of Medical SciencesTehranIran
  2. 2.Cellular and Molecular Research CenterKurdistan University of Medical SciencesSanandajIran
  3. 3.Department of Biology, Parand BranchIslamic Azad UniversityParandIran
  4. 4.Department of Genomic Psychiatry and Behavioral Genomics (DGPBG), Roozbeh Hospital, School of MedicineTehran University of Medical Sciences (TUMS)TehranIran

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