Molecular Biology Reports

, Volume 45, Issue 6, pp 2377–2391 | Cite as

Comparison of two different media for maturation rate of neural progenitor cells to neuronal and glial cells emphasizing on expression of neurotrophins and their respective receptors

  • Reihane Ebadi
  • Dor Mohammad Kordi-TamandaniEmail author
  • Kamran GhaediEmail author
  • Mohammad Hossein Nasr-EsfahaniEmail author
Original Paper


Neural cells derived from embryonic stem cells (ESCs) have potential usefulness for the treatment of neurodegenerative disorders. Modulation of intrinsic growth factors expression such as neurotrophins and their respective receptors by these cells is necessary to obtain functional neural cells for transplantation. In present study, we compared neural differentiation potential of two different media, NB + 5%ES-FBS + N2B27 and Ko-DMEM + 5%ES-FBS for conversion of mESC derived neural progenitors (NPs) into mature neural cells with emphasis on effect of the these two media on neurotrophins and their respective receptors expression. Immunofluorescence staining, RT-qPCR and western blot analysis showed that the expression of neuronal specific markers, MAP2 and Tuj-1, in NB + 5%ES-FBS + N2B27 medium was significantly higher than the other medium. Western blot assay revealed that the expression of BDNF and NGF increased significantly in mature neural cells obtained from NB + 5%ES-FBS + N2B27 medium but decreased in neural cells from Ko-DMEM + 5%ES-FBS medium compared to mESCs. TrkB protein was not detectable in mESCs but its expression increased in neural cells obtained from both media although its expression in NB + 5%ES-FBS + N2B27 medium was significantly higher than the other medium. In contrast to TrkB, p75NTR protein was detectable in mESCs and is remained constant in neural cells cultured in NB + 5%ES-FBS + N2B27 medium but decreased significantly in the other medium. In conclusion, our results indicated that NB + 5%ES-FBS + N2B27 medium promoted neural differentiation process of mESCs and caused enhancement of neurotrophins protein expression in addition to their cognate receptors.


Mouse embryonic stem cell Medium Neural differentiation Neurotrophins Neurotrophin receptors 



Two-way analysis of variance


Brain-derived neurotrophic factor


Basic helix–loop–helix


Bovine serum albumin


Central nervous system


Corrected total cell fluorescence




Dulbecco’s modified Eagle’s medium/Hams F12 medium


Embryoid body


Enhanced chemiluminescence


Extracellular signal-regulated kinase


Embryonic stem cell


Embryonic stem cell qualified fetal bovine serum


Fluorescein isothiocyanate


Glyceraldehyde 3-phosphate dehydrogenase


Glial fibrillary acidic protein


Horse radish peroxidase


Jun N-terminal kinase


Knock-out DMEM


Leukemia inhibitory factor


Microtubule associated protein 2


Mouse embryonic stem cell






Neural cell


Nerve growth factor


Neural progenitor


Neural stem cell








p75 neurotrophin receptor


Phosphate buffered saline




Polyvinylidene difluoride


Retinoic acid


Room temprature


Real-time quantitative polymerase chain reaction


Sodium dodecyl sulfate-polyacrylamide gel electrophoresis


Standard error of the mean


Total RNA isolation


Tropomyosin related kinase



Authors would like to thank University of Sistan and Baluchestan and Royan Institute for the financial support of this project. The authors are thankful to other members of Royan Institute for their excellent technical assistance and advice.

Author contributions

RE experimental design, data collection, data analysis, data interpretation and manuscript writing. DMKT experimental design, financial support, data analysis, data interpretation, and final approval of the manuscript. KG experimental design, data analysis, data interpretation, manuscript writing, and final approval of the manuscript. MHNE experimental design, financial support, data analysis, data interpretation, and final approval of manuscript.

Compliance with ethical standards

Conflict of interest

The authors indicate no potential conflict of interest.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of BiologyUniversity of Sistan and BaluchestanZahedanIran
  2. 2.Department of Biology, Faculty of SciencesUniversity of IsfahanIsfahanIran
  3. 3.Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for BiotechnologyACECRIsfahanIran

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