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Molecular Biology Reports

, Volume 45, Issue 6, pp 2307–2312 | Cite as

Ruthenium pyridyl thiocyanate complex increased the production of pro-inflammatory TNFα and IL1β cytokines by the LPS stimulated mammalian macrophages in vitro

  • Furkan Ayaz
Original Article
  • 51 Downloads

Abstract

Every cell in our body depends on the electron transport processes in order to generate energy and function properly. Being able to regulate the metabolic activity of a cell would enable us altering its function and eventually lead us to a desired biological outcome at the cellular level and more desirably at a systemic level. Immunomodulatory or immunostimulatory molecules have been focus of the recent studies in order to regulate or boost the activities of the immune system cells and suppress or eliminate the disease conditions such as cancer, autoimmune reactions, inflammatory disorders as well as infections. In our study we used a ruthenium pyridyl thiocyanate complex, K330, to examine its effect on the activity of the innate immune system cells, macrophages in vitro. K330 was our candidate due to its application in the solar cells. Especially, due to its ability to get involved in electron transfer systems we hypothesized that it could change the activity of the immune system cells at cellular level, possibly by interfering the electron transfer reactions of the cells. Our results support our hypothesis since K330 lead to a significant increase in TNFα and IL1β cytokine production levels by LPS stimulated macrophages compared to only LPS treated control groups. Based on our in vitro results, K330 can also be utilized as an adjuvant candidate in vaccinations where the antigen itself is not sufficient to generate a proper immune response.

Keywords

TNF-α IL-6 IL-1β Inflammation Macrophage Immunomodulation Adjuvants Innate immunity 

Abbreviations

TNF-α

Tumor nacrosis factor-α

IL-6

Interleukin 6

IL1β

Interleukin 1β

RAW 264.7

Mouse macrophage cell line

ELISA

Enzyme linked immunosorbent assay

LPS

Lipopolysachharide

Notes

Acknowledgements

I greatly appreciate the material supports of Prof. Dr. Kasım Ocakoglu from Tarsus University and Prof. Dr. Juan Anguita from CICBiogune.

Compliance with ethical standards

Conflict of interest

The author declare no competing financial or non-financial conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Biotechnology, Faculty of Arts and ScienceMersin UniversityMersinTurkey

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