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Molecular Biology Reports

, Volume 45, Issue 5, pp 1263–1268 | Cite as

Study of association between interleukin-8 − 845 T/C and + 781 C/T polymorphisms with periodontitis disease among population from Western Iran

  • Mehdi Sajadi
  • Azin Shahmohammadi
  • Sanaz Mahmazi
  • Hoda Bashiri
  • Mehrdad Bavandpour
  • Kheirollah Yari
Original Article
  • 62 Downloads

Abstract

Periodontitis is a chronic inflammatory disease that influences the protective tissues of teeth. IL-8, a member of the chemokine super-family, plays vital roles in the pathogenesis of periodontitis with activation and migration of neutrophils in inflammatory regions. The purpose of present study was to evaluate the association of interleukin-8 − 845 T/C and + 781 C/T polymorphisms with periodontitis in an Iranian population. A total of 65 patients with periodontitis including 18 patients with chronic periodontitis and 47 patients with aggressive periodontitis and 55 controls were enrolled into our study. Interleukin-8 − 845 T/C and + 781 C/T polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. For + 781C/T locus, in the dominant genetic model there was a significant difference between TT vs. CC + CT genotypes that significantly had a protective role against periodontitis disease with a value of 0.38 (95% CI 0.16–0.90, p = 0.02). Also, the analysis of results showed a significant positive association between the distribution of IL-8 − 845 T/C alleles and the risk of periodontitis disease (χ2 = 6.2, p = 0.01) that presence of C allele of IL-8 − 845 increased the risk of periodontitis disease by 9.08-fold [OR 9.08 (95% CI 1.14–72.12, p = 0.03)]. In conclusion, our results demonstrate a positive association between distribution of IL-8 − 845 T/C alleles and risk of periodontitis disease.

Keywords

Periodontitis Interleukin-8 Polymorphism − 845 T/C + 781 C/T 

Notes

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the Ethics Committee of Kermanshah University of Medical Sciences, Iran. All participants signed the informed consent form, in line with the principles of the Helsinki II declarations.

References

  1. 1.
    Flemmig TF (1999) Periodontitis. Ann Periodontol 4:32–38CrossRefPubMedGoogle Scholar
  2. 2.
    Schenkein HA (2006) Host responses in maintaining periodontal health and determining periodontal disease. Periodontol 2000 40:77–93CrossRefPubMedGoogle Scholar
  3. 3.
    Dye BA (2012) Global periodontal disease epidemiology. Periodontol 2000 58:10–25CrossRefPubMedGoogle Scholar
  4. 4.
    Corbet EF, Zee KY, Lo EC (2002) Periodontal diseases in Asia and Oceania. Periodontol 2000 29:122–152CrossRefPubMedGoogle Scholar
  5. 5.
    Armitage GC (2004) Periodontal diagnoses and classification of periodontal diseases. Periodontol 2000 34:9–21CrossRefPubMedGoogle Scholar
  6. 6.
    Genco RJ, Ho AW, Grossi SG, Dunford RG, Tedesco LA (1999) Relationship of stress, distress and inadequate coping behaviors to periodontal disease. J Periodontol 70(7):711–723.  https://doi.org/10.1902/jop.1999.70.1.100 CrossRefPubMedGoogle Scholar
  7. 7.
    Bergstro¨m J (1989) Cigarette smoking as risk factor in chronic periodontal disease. Community Dent Oral Epidemiol 17:245–247CrossRefGoogle Scholar
  8. 8.
    Karimbux NY, Saraiya VM, Elangovan S et al (2012) Interleukin-1 gene polymorphisms and chronic periodontitis in adult whites: a systematic review and metaanalysis. J Periodontol 83:1407–1419CrossRefPubMedGoogle Scholar
  9. 9.
    Ding C, Zhao L, Sun Y, Li L, Xu Y (2012) Interleukin-1 receptor antagonist polymorphism (rs2234663) and periodontitis susceptibility: a meta-analysis. Arch Oral Biol 57(6):585–593.  https://doi.org/10.1016/j.archoralbio.2012.01.016 CrossRefPubMedGoogle Scholar
  10. 10.
    Ding C, Ji X, Chen X, Xu Y, Zhong L (2014) TNF-a gene promoter polymorphisms contribute to periodontitis susceptibility: evidence from 46 studies. J Clin Periodontol 41:748–759CrossRefPubMedGoogle Scholar
  11. 11.
    Bickel M (1993) The role of interleukin-8 in inflammation and mechanisms of regulation. J Periodontol 64(Suppl. 5):456–460PubMedGoogle Scholar
  12. 12.
    Bascones A, Noronha S, Gomez M, Mota P, Gonzalez Moles MA, Villarroel Dorrego M (2005) Tissue destruction in periodontitis: bacteria or cytokines fault? Quintessence Int 36(4):299–306PubMedGoogle Scholar
  13. 13.
    Hull J, Thomson A, Kwiatkowski D (2000) Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. Thorax 55(12):1023–1027CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Zhang N, Xu Y, Zhang B, Zhang T, Yang H, Zhang B, Feng Z, Zhong D (2014) Analysis of Interleukin-8 gene variants reveals their relative importance as genetic susceptibility factor s for chronic periodontitis in the han population. PLoS ONE 9(8):e104436CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Kim YJ, Viana AC, Curtis KM, Orrico SR, Cirelli JA, Scarel-Caminaga RM (2009) Lack of association of a functional polymorphism in the interleukin 8 gene with susceptibility to periodontitis. DNA Cell Biol 28(4):185–190.  https://doi.org/10.1089/dna.2008.0816 CrossRefPubMedGoogle Scholar
  16. 16.
    Andia DC, Letra A, Casarin RC, Casati MZ, Line SR et al (2012) Genetic analysis of the IL-8 gene polymorphism (rs4073) in generalized aggressive periodontitis. Arch Oral Biol 58:211–217CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Rahimi Z, Yari K, Rahimi Z (2015) Matrix metalloproteinase-9-1562T allele and its combination with MMP-2-735 C allele are risk factors for breast cancer. Asian Pac J Cancer Prev 16(3):1175–1179CrossRefPubMedGoogle Scholar
  18. 18.
    Koensgen D, Bruennert D, Ungureanu S, Sofroni D, Braicu EI, Sehouli J, Sümnig A, Delogu S, Zygmunt M, Goyal P, Evert M, Olek S, Biebler KE, Mustea A (2015) Polymorphism of the IL-8 gene and the risk of ovarian cancer. Cytokine 71(2):334–338CrossRefPubMedGoogle Scholar
  19. 19.
    Atalay A, Arıkan S, Ozturk O, Öncü M, Tasli ML, Duygulu S, Atalay EO (2016) The IL-8 gene polymorphisms in Behçet’s disease observed in Denizli province of Turkey. Immunol Invest 45(4):298–311CrossRefPubMedGoogle Scholar
  20. 20.
    Yari K, Payandeh M, Rahimi Z (2016) Association of the hypermethylation status of PTEN tumor suppressor gene with the risk of breast cancer among Kurdish population from Western Iran. Tumor Biol 37:8145CrossRefGoogle Scholar
  21. 21.
    Campa D, Hung RJ, Mates D, Zaridze D, Szeszenia-Dabrowska N et al (2005) Lack of association between 2251 TNA polymorphism of IL8 and lung cancer risk. Cancer Epidemiol Biomarkers Prev 14:2457–2458CrossRefPubMedGoogle Scholar
  22. 22.
    Fey MF, Tobler A (1993) An interleukin-8 (IL-8) cDNA clone identifis a frequent HindIII polymorphism. Hum Genet 91(3):298CrossRefPubMedGoogle Scholar
  23. 23.
    Renzoni E, Lympany P, Sestini P et al (2000) Distribution of novel polymorphisms of the interleukin-8 and CXC receptor 1 and 2 genes in systemic sclerosis and cryptogenic firosing alveolitis. Arthritis Rheum 43(7):1633–1640CrossRefPubMedGoogle Scholar
  24. 24.
    Hull J, Thmson A, Kwiatkowski D (2000) Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. Thorax 55(12):1023–1027CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Rovin BH, Lu L, Zhang X (2002) A novel interleukin-8 polymorphism is associated with severe systemic lupus erythematosus nephritis. Kidney Int 62(1):261–265CrossRefPubMedGoogle Scholar
  26. 26.
    Kim YJ, Viana AC, Curtis KM, Orrico SR, Cirelli JA et al (2010) Association of haplotypes in the IL-8 gene with susceptibility to chronic periodontitis in a Brazilian population. Clin Chim Acta 411:1264–1268CrossRefPubMedGoogle Scholar
  27. 27.
    Scarel-Caminaga RM, Kim YJ, Viana AC, Curtis KM, Corbi SC et al (2011) Haplotypes in the interleukin 8 gene and their association with chronic periodontitis susceptibility. Biochem Genet 49:292–302CrossRefPubMedGoogle Scholar
  28. 28.
    Andia DC, de Oliveira NF, Letra AM, Nociti FH Jr, Line SR et al (2011) Interleukin-8 gene promoter polymorphism (rs4073) may contribute to chronic periodontitis. J Periodontol 82:893–899CrossRefPubMedGoogle Scholar
  29. 29.
    Corbi SC, Anovazzi G, Finoti LS, Kim YJ, Capela MV et al (2012) Haplotypes of susceptibility to chronic periodontitis in the Interleukin 8 gene do not influence protein level in the gingival crevicular fluid. Arch Oral Biol 57:1355–1361CrossRefPubMedGoogle Scholar
  30. 30.
    Andia DC, Letra A, Casarin RC, Casati MZ, Line SR, de Souza AP (2013) Genetic analysis of the IL8 gene polymorphism(rs4073) in generalized aggressive periodontitis. Arch Oral Biol 58(2):211–217CrossRefPubMedGoogle Scholar
  31. 31.
    Borrell LN, Papapanou PN (2005) Analytical epidemiology of periodontitis. J Clin Periodontol 32(Suppl 6):132–158.  https://doi.org/10.1111/j.1600-051X.2005.00799.x CrossRefPubMedGoogle Scholar
  32. 32.
    Houshmand B, Hajilooi M, Rafii A, Bidgoli M, Soheilifar S (2012) Evaluation of IL-8 gene polymorphisms in patients with periodontitis in Hamedan, Iran. Dental Res J 9(4):427–432Google Scholar
  33. 33.
    Linhartova P, Vokurka J, Poskerova H, Fassmann A, Holla LI (2013) Haplotype analysis of interleukin-8 gene polymorphisms in chronic and aggressive periodontitis. Mediat Infammation 342351Google Scholar
  34. 34.
    Sippert EA, de Oliveira e Silva C, Visentainer JE, Sell AM (2013) Association of duffy blood group gene polymorphisms with IL8 gene in chronic periodontitis. PLoS ONE 8(12):e83286.  https://doi.org/10.1371/journal.pone.0083286 CrossRefPubMedPubMedCentralGoogle Scholar
  35. 35.
    Chen X, Huang J, Zhong L, Ding C (2015) Quantitative assessment of the associations between interleukin-8 polymorphisms and periodontitis susceptibility. J Periodontol 86(Number 2):292–300CrossRefPubMedGoogle Scholar
  36. 36.
    da Silva MK, de Carvalho ACG, Alves EHP, da Silva FRP, Pessoa LDS, Vasconcelos DFP (2017) Genetic factors and the risk of periodontitis development: findings from a systematic review composed of 13 studies of meta-analysis with 71,531 participants. Int J Dent.  https://doi.org/10.1155/2017/1914073 CrossRefPubMedPubMedCentralGoogle Scholar
  37. 37.
    Ni X-B, Jia C, Yu H-D, Li Y-Q, Zeng X-T, Leng W-D (2017) Comprehensive analysis of interleukin-8 gene polymorphisms and periodontitis susceptibility. Oncotarget 8:48996PubMedPubMedCentralGoogle Scholar
  38. 38.
    Ayazi G, Pirayesh M, Yari K (2013) Analysis of interleukin-1 b gene polymorphism and its association with generalized aggressive periodontitis disease. DNA Cell Biol 32(Number 7):1–5CrossRefGoogle Scholar
  39. 39.
    Kazemi E, Moradi M-T, Yari K, Mousavi SAR, Kahrizi D (2015) Association between manganese superoxide dismutase (MnSOD Val-9Ala) genotypes with the risk of generalized aggressive periodontitis disease. Cell Mol Biol 61(8):49–52PubMedGoogle Scholar
  40. 40.
    Darvishi E, Aziziaram Z, Yari K, Bagheri Dehbaghi M, Kahrizi D, Karim H, Vaziri S, Zargooshi J, Ghadiri K, Muhammadi S, Kazemi E, Moradi MT, Shokrinia M, Mohammadi N (2016) Lack of association between the TNF-α-1031genotypes and generalized aggressive periodontitis disease. Cell Mol Biol 62(11):62–65Google Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Mehdi Sajadi
    • 1
  • Azin Shahmohammadi
    • 2
  • Sanaz Mahmazi
    • 1
  • Hoda Bashiri
    • 3
  • Mehrdad Bavandpour
    • 1
  • Kheirollah Yari
    • 2
    • 3
  1. 1.Department of Genetics, Faculty of Basic SciencesIslamic Azad UniversityZanjanIran
  2. 2.Medical Biology Research CenterKermanshah University of Medical SciencesKermanshahIran
  3. 3.Zagros Bioidea Co.Razi University IncubatorKermanshahIran

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