Molecular Biology Reports

, Volume 45, Issue 6, pp 1575–1585 | Cite as

Polymorphism distribution of RYR1, PRKAG3, HFABP, MYF-5 and MC4R genes in crossbred pigs

  • B. L. SainiEmail author
  • G. K. Gaur
  • N. R. Sahoo
  • S. K. Mendiratta
  • A. Kumar
  • B. C. Naha
  • A. Baranwal
  • V. Yadav
  • R. K. Jaiswal
Original Article


This study was designed to screen the crossbred pigs for SNPs in five candidate genes, associated with pork quality traits and to differentiate their genotypes by PCR–RFLP. The results indicated that genotypes of crossbred pigs were NN (90%) and Nn (10%) for RYR1; RR (83%) and QR (17%) for PRKAG3; HH (98%), Hh (1%) and hh (1%) for HFABP; DD (99%) and CD (1%) for MYF-5; and AG (57%), GG (26%) and AA (17%) for MC4R SNPs, respectively. Allelic frequencies for five SNPs {RYR1 (1843C>T), PRKAG3 (c.599G>A), HFABP (c.1322C>T), MYF-5 (c.1205A>C) and MC4R (c.1426A>G)} were 0.95 and 0.05 (N/n), 0.08 and 0.92 (Q/R), 0.99 and 0.01 (H/h), 0.00 and 1.00 (C/D) and 0.45 and 0.55 (A/G), respectively. The effect of RYR1 (1843C>T) SNP was significant on pH45 (P < 0.05), pH24 (P < 0.05) and protein % (P < 0.05). The PRKAG3 (c.599G>A) and MC4R (c.1426A>G) SNP had significant association with dressing percentages. The results revealed that RYR1, PRKAG3 and MC4R SNPs may be used in marker associated selection for pork quality traits in crossbred pigs.


Crossbred pig PCR–RFLP SNP Pork quality 



The help rendered by Director, Joint Director (Research) and Joint Director (Academic); Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh and financial support by Indian Council of Agricultural Research, New Delhi is highly acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests for this publication.


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© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Division of Animal GeneticsICAR-IVRIIzatnagarIndia
  2. 2.Livestock Production and Management SectionICAR-IVRIIzatnagarIndia
  3. 3.Division of Livestock Products TechnologyICAR-IVRIIzatnagarIndia
  4. 4.Dairy Cattle Breeding DivisionICAR-NDRIKarnalIndia

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