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Immunomodulatory effect of mesenchymal stem cells: Cell origin and cell quality variations

  • Marwa El-SayedEmail author
  • Mohamed Ali El-Feky
  • Mostafa I. El-Amir
  • Al Shaimaa Hasan
  • Mohammed Tag-Adeen
  • Yoshishige Urata
  • Shinji Goto
  • Lan Luo
  • Chen Yan
  • Tao-Sheng Li
Original Article
  • 46 Downloads

Abstract

The immunomodulatory property of mesenchymal stem cells (MSCs) has been previously reported. Still it is unclear if this property can be affected by the cell origin and cell quality. Using primary MSCs expanded from bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of mice, we investigated whether the immunomodulatory property of MSCs varied with cell origin and cell quality (early- vs. late-passaged BM-MSCs). BM-MSCs (p1) and AD-MSCs (p1) had a typical spindle shape, but morphological changes were observed in late-passaged BM-MSCs (p6). A pathway-focused array showed that the expression of chemokine/cytokine genes varied with different cell origins and qualities. By co-culturing with spleen mononuclear cells (MNC) for 3 days, the expression of CD4 was suppressed by all types of MSCs. By contrast, the expression of CD8 was suppressed by BM-MSCs and increased by AD-MSCs. The expression ratio of CD206 to CD86 was at a comparable level after co-culture with AD-MSCs and BM-MSCs, but was lower with late-passaged BM-MSCs. AD-MSCs highly induced the release of IL6, IL-10 and TGF-β in culture medium. Compared with early-passaged BM-MSCs (p1), late-passaged BM-MSCs (p6) released less TGF-β. Our data suggests that the immunomodulatory properties of MSCs vary with cell origin and cell quality and that BM-MSCs of good quality are likely the optimal source of immunomodulation.

Keywords

Mesenchymal stem cell BM-MSC AD-MSC Immunomodulation T cell Macrophage 

Notes

Funding

This study was supported by the Ministry of Higher Education, Egypt and a Grant-in-Aid from the Ministry of Education, Science, Sports, Culture and Technology, Japan.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Ethics approval

All experiments were approved by the Institutional Animal Care and Use Committee of Nagasaki University and performed in accordance with the institutional and national guidelines.

Supplementary material

11033_2018_4582_MOESM1_ESM.doc (5.7 mb)
Supplementary material 1 (DOC 5881 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Marwa El-Sayed
    • 1
    • 2
    Email author return OK on get
  • Mohamed Ali El-Feky
    • 2
    • 3
  • Mostafa I. El-Amir
    • 2
  • Al Shaimaa Hasan
    • 1
    • 4
  • Mohammed Tag-Adeen
    • 5
    • 6
  • Yoshishige Urata
    • 1
  • Shinji Goto
    • 1
  • Lan Luo
    • 1
  • Chen Yan
    • 1
  • Tao-Sheng Li
    • 1
  1. 1.Department of Stem Cell BiologyNagasaki University Graduate School of Biomedical SciencesNagasakiJapan
  2. 2.Department of Microbiology and Immunology, Faculty of MedicineSouth Valley UniversityQenaEgypt
  3. 3.Department of Microbiology and Immunology, Faculty of MedicineAssiut UniversityAssiutEgypt
  4. 4.Department of Pharmacology, Faculty of MedicineSouth Valley UniversityQenaEgypt
  5. 5.Internal Medicine Department, Faculty of MedicineSouth Valley UniversityQenaEgypt
  6. 6.Hepatology and Gastroenterology DepartmentNagasaki University HospitalNagasakiJapan

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