As one of the important traits, hull color is the morphological marker of rice, which plays an important role in the mechanized color selection of hybrid rice seed production but lacks good application. Here, we obtained a reddish-brown hull 1 (rbh1) mutant from an Indica maintainer material H9808 by aerospace mutagenesi. In the rbh1 mutant, the hull color was reddish brown, and the grain width and 1000-grain weight decreased significantly, but the other agronomic traits did not change significantly. Furthermore, the total flavonoids and anthocyanin content in the rbh1 hulls deposition was remarkably higher than WT, and the lignin level in the rbh1 hull was reduced. Genetic analysis indicated that the reddish-brown hull trait was controlled by a pair of recessive nuclear genes. Map-based cloning indicated that RBH1 was located within the physical distance of 48 kb on the short arm of chromosome 2. The comparative analysis of genome DNA sequence between rbh1 and WT found that a substitution from T to C (+ 1001) occurred in the fourth exon of LOC_Os02g09490 in rbh1 mutant. Genetic complementation experiments indicate that RBH1 is an allele of the previously reported GH2, which encoding a cinnamyl alcohol dehydrogenase protein involving in lignin biosynthesis. qRT-PCR showed that the relative expression of lignin and flavonoid-related genes in the hulls of rbh1 mutant was significantly upregulated, confirming that GH2/RBH1 is an important gene in the metabolism of lignin and flavonoids; and provides material basis for further studying the mechanism of GH2/RBH1 regulating the rice hull color.. In addition, the hybrid F1 combination analysis indicated that the rbh1 mutation site did not affect the agronomic traits and yield of the hybrid rice, which was to cultivate the rbh1 locus into a new sterile line or restorer line with reddish-brown hulls and rice breeding application of rbh1 locus on mechanized seed production of hybrid rice provides a theoretical basis.
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Bariola PA, MacIntosh GC, Green PJ (1999) Regulation of S-like ribonuclease levels in Arabidopsis. Antisense inhibition of RNS1 or RNS2 elevates anthocyanin accumulation. Plant Physiol 119(1):331–342
Barrière Y, Ralph J, Méchin V, Guillaumie S, Grabber JH, Argillier O, Chabbert B, Lapierre C (2004) Genetic and molecular basis of grass cell wall biosynthesis and degradability. II Lessons from brown-midrib mutants Comptes rendus biologies 327(9–10):847–860
Baucher M, Chabbert B, Pilate G, Van Doorsselaere J, Tollier M-T, Petit-Conil M, Cornu D, Monties B, Van Montagu M, Inze D (1996) Red xylem and higher lignin extractability by down-regulating a cinnamyl alcohol dehydrogenase in poplar. Plant Physiol 112(4):1479–1490
Baucher M, Bernard-Vailhé MA, Chabbert B, Besle J-M, Opsomer C, Van Montagu M, Botterman J (1999) Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility. Plant Mol Biol 39(3):437–447
Boerjan W, Ralph J, Baucher M (2003) Lignin biosynthesis. Annu Rev Plant Biol 54(1):519–546
Campbell MM, Sederoff RR (1996) Variation in lignin content and composition (mechanisms of control and implications for the genetic improvement of plants). Plant Physiol 110(1):3–13
Cherney J, Cherney D, Akin D, Axtell J (1991) Potential of brown-midrib, low-lignin mutants for improving forage quality. In: advances in agronomy. Elsevier 46:157–198
Ferrer J-L, Austin M, Stewart C Jr, Noel J (2008) Structure and function of enzymes involved in the biosynthesis of phenylpropanoids. Plant Physiol Biochem 46(3):356–370
Fitzgerald MA, McCouch SR, Hall RD (2009) Not just a grain of rice: the quest for quality. Trends Plant Sci 14(3):133–139
Furukawa T, Maekawa M, Oki T, Suda I, Iida S, Shimada H, Takamure I, Ki K (2007) The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp. Plant J 49(1):91–102
Gu X-Y, Kianian SF, Hareland GA, Hoffer BL, Foley ME (2005) Genetic analysis of adaptive syndromes interrelated with seed dormancy in weedy rice (Oryza sativa). Theor Appl Genet 110(6):1108–1118
He L-b, L-y C, Qian Q (2001) The prospects of hybrid rice seed production by using rice chaff colour marker. ACTA AGRICULTURAE ZHEJIANGENSIS 13(6):357–360
Hirano K, Aya K, Kondo M, Okuno A, Morinaka Y, Matsuoka M (2012) OsCAD2 is the major CAD gene responsible for monolignol biosynthesis in rice culm. Plant Cell Rep 31(1):91–101
Hong L, Qian Q, Tang D, Wang K, Li M, Cheng Z (2012) A mutation in the rice chalcone isomerase gene causes the golden hull and internode 1 phenotype. Planta 236(1):141–151
Humphreys JM, Chapple C (2002) Rewriting the lignin roadmap. Curr Opin Plant Biol 5(3):224–229
Johnson D (1961) The spectrophotometric determiantion of lignin in small wood samples. Tappi 44:793–798
Koes R, Verweij W, Quattrocchio F (2005) Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. Trends Plant Sci 10(5):236–242
Koshiba T, Murakami S, Hattori T, Mukai M, Takahashi A, Miyao A, Hirochika H, Suzuki S, Sakamoto M, Umezawa T (2013) CAD2 deficiency causes both brown midrib and gold hull and internode phenotypes in Oryza sativa L. cv. Nipponbare. Plant biotechnol 13:0527
Liu L, Tang W, Yu X, Liu Y (2012) Studies on methods of agrobacterium-mediated transformation of Indica Rice (Shuhui 527). Journal of Agricultural Science and Technology (Beijing) 14(4):135–141
MacKay JJ, O’Malley DM, Presnell T, Booker FL, Campbell MM, Whetten RW, Sederoff RR (1997) Inheritance, gene expression, and lignin characterization in a mutant pine deficient in cinnamyl alcohol dehydrogenase. Proc Natl Acad Sci 94(15):8255–8260
M-e T (1957) Analysis on apiculus color genes essential to anthocyanin coloration rice. Journal of the Faculty of Agriculture, Hokkaido University 50(3):266–362
Nagao S (1951) Genie analysis and linkage relationship of characters in rice. In: advances in genetics. Elsevier 4:181–212
Ookawa T, Inoue K, Matsuoka M, Ebitani T, Takarada T, Yamamoto T, Ueda T, Yokoyama T, Sugiyama C, Nakaba S (2014) Increased lodging resistance in long-culm, low-lignin gh2 rice for improved feed and bioenergy production. Sci Rep 4:6567
Reddy A (1996) Genetic and molecular analysis of the anthocyanin pigmentation pathway in rice. In: Khush G,Hettel G, Rola T (eds) Rice Genetics III: (In 2 Parts). World Scientific, pp 341–352
Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5(2):69–76
Saitoh K, Onishi K, Mikami I, Thidar K, Sano Y (2004) Allelic diversification at the C (OsC1) locus of wild and cultivated rice: nucleotide changes associated with phenotypes. Genetics 168(2):997–1007
Sattler SE, Saathoff AJ, Haas EJ, Palmer NA, Funnell-Harris DL, Sarath G, Pedersen JF (2009) A nonsense mutation in a cinnamyl alcohol dehydrogenase gene is responsible for the sorghum brown midrib6 phenotype. Plant Physiol 150(2):584–595
Shao T, Qian Q, Tang D, Chen J, Li M, Cheng Z, Luo Q (2012) A novel gene IBF1 is required for the inhibition of brown pigment deposition in rice hull furrows. Theor Appl Genet 125(2):381–390
Sibout R, Eudes A, Mouille G, Pollet B, Lapierre C, Jouanin L, Séguin A (2005) CINNAMYL ALCOHOL DEHYDROGENASE-C and-D are the primary genes involved in lignin biosynthesis in the floral stem of Arabidopsis. Plant Cell 17(7):2059–2076
Sweeney MT, Thomson MJ, Pfeil BE, McCouch S (2006) Caught red-handed: Rc encodes a basic helix-loop-helix protein conditioning red pericarp in rice. Plant Cell 18(2):283–294
Tobias CM, Chow EK (2005) Structure of the cinnamyl-alcohol dehydrogenase gene family in rice and promoter activity of a member associated with lignification. Planta 220(5):678–688
Wyrambik D, Grisebach H (1975) Purification and properties of isoenzymes of cinnamyl-alcohol dehydrogenase from soybean-cell-suspension cultures. Eur J Biochem 59(1):9–15
Xia X, Xiao-bo Z, Yong-feng S, Hui-mei W, Bao-hua F, Xiao-hong L, Qi-na H, Li-xin S, Dan G, Yan H (2016) A point mutation in an F-box domain-containing protein is responsible for brown hull phenotype in rice. Rice Sci 23(1):1–8
Yang C, Zeng D, Qin R, Alamin M, Jin X, Shi C (2018) Rice gene, BBH/Lsi1, regulates the color of rice hull by reducing the absorption and deposition of silicon and accumulating excess flavonoid. Plant Growth Regul 85(1):133–142
Zeng D-L, Qian Q, Dong G-J, Zhu X-D, Dong F-G, Teng S, Guo L-B, Cao L-Y, Cheng S-H, Xiong Z-M (2003) Development of isogenic lines of morphological markers in indica rice. ACTA BOTANICA SINICA-CHINESE EDITION 45(9):1116–1120
Zhang K, Qian Q, Huang Z, Wang Y, Li M, Hong L, Zeng D, Gu M, Chu C, Cheng Z (2006) GOLD HULL AND INTERNODE2 encodes a primarily multifunctional cinnamyl-alcohol dehydrogenase in rice. Plant Physiol 140(3):972–983
Zhu B-F, Si L, Wang Z, Zhu YZJ, Shangguan Y, Lu D, Fan D, Li C, Lin H, Qian Q (2011) Genetic control of a transition from black to straw-white seed hull in rice domestication. Plant Physiol 155(3):1301–1311
This research was supported by the National Key Technology Research and Development Program of China (2016YFD0102102 to Ping Wang), the Sichuan Innovation Ability Rising Government Financial Project (2018QNJJ-026 to Binhua Hu), and Sichuan Science and Technology Program (2017JY0280 to Xiaoli Xiang).
The authors declare that they have no competing interests.
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Wang, P., Hu, B., Xiang, X. et al. A single nucleotide mutation in the fourth exon of RBH1 is responsible for brown hull phenotype in rice. Mol Breeding 40, 22 (2020). https://doi.org/10.1007/s11032-020-1099-8
- Oryza sativa L
- Reddish brown hull
- Mechanization of hybrid seed production