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Molecular Breeding

, 39:144 | Cite as

Characterization of a new sg-5 variant with reduced biosynthesis of group A saponins in soybean (Glycine max (L.) Merr.)

  • Jagadeesh Sundaramoorthy
  • Sampathkumar Palaniswamy
  • Gyu Tae Park
  • Hae Reon Son
  • Chigen Tsukamoto
  • Jeong-Dong Lee
  • Jeong Hoe Kim
  • Hak Soo Seo
  • Jong Tae SongEmail author
Article
  • 38 Downloads

Abstract

Triterpenoid saponins are major secondary metabolites in soybean; they are classified into group A and DDMP saponins. Group A saponins cause bitterness and an astringent aftertaste, whereas DDMP saponins are less bitter and more beneficial to human health. Therefore, a need exists for genetic improvement to develop bitter-free soybeans without losing other beneficial saponins. In this study, an ethyl methanesulfonate (EMS)–induced mutant, PE1327, was isolated and characterized as having a reduced level of group A saponins by chromatography. The PE1327 phenotype, which exhibits no accumulation of group A saponins, resembles that of a previously reported sg-5 wild soybean mutant whose biosynthesis is controlled by the Sg-5 (Glyma.15G243300) gene. The Sg-5 gene sequence in PE1327 revealed a single-nucleotide polymorphism (G1036A) that causes an amino acid change from Glu346 to Lys346 in the sg-5 protein. Multiple alignment analysis of cytochrome P450 enzymes from numerous organisms revealed that Glu346 is highly conserved in eukaryotes, including plants and animals, but not in prokaryotes. The predicted 3D model of the Sg-5 protein showed that the Glu346 residue is located in the J helix and is likely involved in the linkage between the J and K helices and the stabilization of the J-K loop, suggesting that the amino acid substitution from Glu346 to Lys346 in the PE1327 mutant results in hypofunction of Sg-5. Co-segregation analysis revealed that the Sg-5 locus is tightly linked to reduced group A saponin biosynthesis in PE1327 and that the novel sg-5 variant is recessive to Sg-5.

Keywords

Soybean Saponin Sg-5 CYP72A69 Group A saponin 

Notes

Funding information

This work was carried out with the support of “Cooperative Research Program for Agricultural Science and Technology Development (Project No. PJ01327602),” Rural Development Administration, Korea.

Supplementary material

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.School of Applied BiosciencesKyungpook National UniversityDaeguSouth Korea
  2. 2.Food Science, Bioresources Sciences, United Graduate School of Agricultural SciencesIwate UniversityMoriokaJapan
  3. 3.Department of BiologyKyungpook National UniversityDaeguSouth Korea
  4. 4.Department of Plant ScienceSeoul National UniversitySeoulSouth Korea

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