Development of a highly specific co-dominant marker for genotyping the Ph-3 (tomato late blight resistance) locus by comparing cultivated and wild ancestor species
- 130 Downloads
Late blight is a devastating disease for tomato especially in areas with high humidity and low temperature caused by Phytophthora infestans. A late blight resistance gene, Ph-3, has been widely used in tomato breeding program as it confers incomplete dominant resistance to a wide range of P. infestans isolates of tomato. This gene was derived from a wild ancestor species of cultivated tomato, Solanum pimpinellifolium accession L3708, and located in a resistance (R) gene cluster. Although this gene was cloned a few years ago, and some markers have been developed and used, the effectiveness of these markers was not evaluated with a diverse cultivars panel. Based on the comparative analysis of the Ph-3 locus sequences from L3708, cultivar Heinz1706 and S. pimpinellifolium accession LA1589, we developed a robust co-dominant PCR-based marker Ph-3-GLR/S for the Ph-3 locus. We performed a comparison about efficiency and accuracy of Ph-3-GLR/S with two cleaved amplified polymorphic sequence (CAPS) markers Ph3.gsm/HincII and TG328 developed previously. Ph-3-GLR/S exhibited robust co-dominant patterns compared to Ph3.gsm/HincII. For certain accessions, TG328 and Ph-3-GLR/S yielded the contrast genotyping result. To clarify this discrepancy, disease resistance evaluation by detached leaf inoculation supported the consistency between Ph-3-GLR/S genotyping and resistance phonotype, showing Ph-3-GLR/S was more accurate than TG328. All these findings indicated that our marker Ph-3-GLR/S could serve as a highly specific and robust co-dominant marker for marker-assisted selection of Ph-3.
KeywordsTomato Molecular marker Late blight Phytophthora infestans Ph-3 Resistance gene
We are grateful to Dr. Jinshuai Shu for providing the tomato race T1,2,4 of P. infestans and to Xiaoling Yu for guidance on the detached leaf inoculation. We also thank Prof. Tingting Huang for kindly providing the seeds of Wanxia.
This project was supported by the grants of the National Key R&D Program of China (2017YFD0101902), China Agriculture Research System (CARS-23-A-03).
- AVDRC (1994) Tomato pathology. In: AVRDC 1993 Progress Report. Asian Vegetable Research and Development Center, Taiwan(ROC), pp 201–203Google Scholar
- Dianese EC, de Fonseca MEN, Goldbach R, Kormelink R, Inoue-Nagata AK, Resende RO, Boiteux LS (2010) Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 (Tospovirus resistance) gene cluster in a wide range of tomato accessions. Mol Breed 25:133–142. https://doi.org/10.1007/s11032-009-9313-8 CrossRefGoogle Scholar
- Feng L, Yang Y, Xie B, Feng D, Yang C (2004) Identification of physiological races of Phytophthora infestans on tomato in eighteen provinces of China. Acta Hortic Sin 31:758–761. https://doi.org/10.16420/j.issn.0513-353x.2004.06.013 CrossRefGoogle Scholar
- Hanson P, Lu S, Wang J, Chen W, Kenyon L, Tan C, Tee KL, Wang Y, Hsu YY, Schafleitner R (2016) Conventional and molecular marker-assisted selection and pyramiding of genes for multiple disease resistance in tomato. Sci Hortic 201:346–354. https://doi.org/10.1015/j.scienta.2016.02.020 CrossRefGoogle Scholar
- Huang T, Liu B, Liu S, Li P, Zhang Y, Sun Z (2011) A new tomato hybrid Wanxia resistant to tomato late blight. Acta Hortic Sin 38:1413–1414. https://doi.org/10.16420/j.issn.0513-353x.2011.07.029 CrossRefGoogle Scholar
- Kong F, Li D, Xu X, Jiang J, Li J (2015) Creation of multi-resistant and storage tolerant tomato material. China Veget 9:20–25Google Scholar
- Li M (2017) The winter control of tomato late blight. China Veget 2:85–86Google Scholar
- Nicholas KB (1997) GeneDoc: analysis and visualization of genetic variation. EMBnet News 4:14Google Scholar
- Peirce L (1971) Linkage tests with Ph conditioning resistance to race 0, Phytophthora infestans. Rep Tomato Genet Coop 21:30Google Scholar
- Seah S, Williamson VM, Garcia BE, Mejía L, Salus MS, Martin CT, Maxwell DP (2007) Evaluation of a co-dominant SCAR marker for detection of the Mi-1 locus for resistance to root-knot nematode in tomato germplasm. Rep Tomato Genet Coop 57:37–40Google Scholar
- Simons G, Groenendijk J, Wijbrandi J, Reijans M, Groenen J, Diergaarde P, Van der Lee T, Bleeker M, Onstenk J, de Both M (1998) Dissection of the Fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy. Plant Cell 10:1055–1068. https://doi.org/10.1105/tpc.10.6.1055 CrossRefPubMedPubMedCentralGoogle Scholar
- Thomas CM, Jones DA, Parniske M, Harrison K, Balint-Kurti PJ, Hatzixanthis K, Jones J (1997) Characterization of the tomato Cf-4 gene for resistance to Cladosporium fulvum identifies sequences that determine recognitional specificity in Cf-4 and Cf-9. Plant Cell 9:2209–2224. https://doi.org/10.1105/tpc.9.12.2209 CrossRefPubMedPubMedCentralGoogle Scholar
- Van der Hoorn RA, Kruijt M, Roth R, Brandwagt BF, Joosten MH, De Wit PJ (2001) Intragenic recombination generated two distinct Cf genes that mediate AVR9 recognition in the natural population of Lycopersicon pimpinellifolium. Proc Natl Acad Sci U S A 98:10493–10498. https://doi.org/10.1073/pnas.181241798 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhang C, Liu L, Wang X, Vossen J, Li G, Li T, Zheng Z, Gao J, Guo Y, Visser RG (2014) The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans. Theor Appl Genet 127:1353–1364. https://doi.org/10.1007/s00122-014-2303-1 CrossRefPubMedPubMedCentralGoogle Scholar