Advertisement

Molecular Breeding

, 39:32 | Cite as

Development of 1AS.1AL-1DL durum wheat chromosome carrying Glu-D1a locus encoding high molecular weight glutenin subunits 2 + 12

  • Olivier CoritonEmail author
  • Annie Faye
  • Etienne Paux
  • Jocelyne Lemoine
  • Virginie Huteau
  • Gerard Branlard
  • Joseph Jahier
Article

Abstract

High molecular weight glutenin subunits (HMW-GS) are well-known to directly influence the bread wheat quality. In a previous paper, our group reported the replacement of the locus Glu-A1 by the locus Glu-D1—allele Glu-D1a encoding the HMW-GS (2–12)—in a partial isohomoeoallelic lines of the French bread wheat variety cv. Courtot. We plan to exploit the novel translocated 1AS.1AL-1DL chromosome in durum wheat in order to develop durum lines presenting novel properties. The translocated chromosome was first introduced into durum wheat. However, as the distal translocated 1DL segment represents 25% (i.e., 84 Mb) of the recombinant 1AL length, it appeared worthwhile to shorten it through homoeologous recombination induced by the ph1c mutation. Two rounds of recombination were made. The shortest interstitial 1DL segment detected through SNP genotyping measured 9.19 Mb and could not be visualized by genomic in situ hybridization. Such reduced transfer not previously achieved hitherto provides regularity of the meiotic pairing and will allow the use of those subunits absent in durum wheat species for direct exploitability in breeding.

Keywords

Durum wheat High molecular weight glutenin subunit Translocation GISH 

Notes

Acknowledgements

This project was supported by the CASDAR project. We thank the UMR INRA 1095 “GENTYANE platform” (Clermont-Ferrand, France, http://gentyane.clermont.inra.fr/) for the generation of genotyping data.

Author contributions

JJ and GB conceived and designed research. AF, JL, and VH conducted experiments. EP contributed to analytical tools. JJ, GB, and OC analyzed data. JJ and OC wrote the manuscript. All authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Blanco A, Cenci A, Simeone R, Gadaleta A, Pignone D, Galasso I (2002) The cytogenetics and molecular characteristics of a translocated chromosome 1AS.1AL-1DL with a Glu-D1 locus in durum wheat. Cell Mol Biol Lett 7:559–567PubMedGoogle Scholar
  2. Branlard G, Dardevet M, Saccomano R, Lagoutte F, Gourdon J (2001) Genetic diversity of wheat storage proteins and bread wheat quality. Euphytica 119:59–67CrossRefGoogle Scholar
  3. Dumur J, Branlard G, Tanguy AM, Dardevet M, Coriton O, Huteau V, Lemoine J, Jahier J (2009) Development of isohomoeoallelic lines within the wheat cv. Courtot for high molecular weight glutenin subunits: transfer of the Glu-D1 locus to chromosome 1A. Theor Appl Genet 119:471–481CrossRefGoogle Scholar
  4. Dumur J, Jahier J, Dardevet M, Chiron H, Tanguy AM, Branlard G (2010) Effects of the replacement of the Glu-A1 by Glu-D1 locus on agronomic performance and bread-making quality of the hexaploid wheat cv. Courtot. J Cereal Sci 51:175–181CrossRefGoogle Scholar
  5. Gennaro A, Forte P, Panichi D, Lafiandra D, Pagnotta MA, D’Egidio MG, Ceoloni C (2012) Stacking small segments of the 1D chromosome of bread wheat containing major gluten quality genes into durum wheat: transfer strategy and breeding prospects. Mol Breeding 30:149–167CrossRefGoogle Scholar
  6. Giorgi B (1978) A homoeologous pairing mutant isolated in Triticum durum cv. Cappelli. Mutat Breed Newsl 11:4–5Google Scholar
  7. IWGSC (The International Wheat Genome Sequencing Consortium) (2018) Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science 361:661Google Scholar
  8. Joppa LR, Klindworth DL, Hareland GA (1998) Transfer of high molecular weight glutenins from spring wheat to durum wheat. In: Slinkard AE (ed) Proceedings of the Ninth International Wheat Genetic Symposium. University of Saskatchewan Ext. Press, Saskatoon, pp 257–260Google Scholar
  9. Lukaszewski AJ (2003) Registration of six germplasms of durum wheat with introgressions of the Glu-D1locus. Crop Sci 43:1138–1139CrossRefGoogle Scholar
  10. Lukaszewski AJ, Lapinski B, Rybka K (2005) Limitations of in situ hybridization with total genomic DNA in routine screening for alien introgressions in wheat. Cytogenetic and Genome Research 109 (1-3):373–377CrossRefGoogle Scholar
  11. Lukaszewski AJ, Curtis CA (1994) Transfer of the Glu-D1 gene from chromosome 1D to chromosome 1A in hexaploid triticale. Plant Breed 112:177–182CrossRefGoogle Scholar
  12. Naranjo T, Roca A, Giraldez R, Goicoechea PG (1988) Chromosome pairing in hybrids of ph1b mutant wheat with rye. Genome 30:639–646CrossRefGoogle Scholar
  13. Payne P (1987) Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu Rev Plant Physiol 38:141–153CrossRefGoogle Scholar
  14. Pestsova E, Ganal M, Röder M (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697CrossRefGoogle Scholar
  15. Rimbert H, Darrier B, Navarro J, Kitt J, Choulet F, Leveugle M, Duarte J, Rivière N, Eversole K, Le Gouis J, Davassi A, Balfourier F, Le Paslier MC, Berard A, Brunel D, Feuillet C, Poncet C, Sourdille P, Paux E (2018) High throughput SNP discovery and genotyping in hexaploid wheat. PLoS One 13(1):e0186329.  https://doi.org/10.1371/journal.pone.0186329 CrossRefPubMedPubMedCentralGoogle Scholar
  16. Rogers W, Rickatson J, Sayers E, Law C (1990) Dosage effects of chromosomes of homoeologous groups 1 and 6 upon bread-making quality in hexaploid wheat. Theor Appl Genet 80:281–287CrossRefGoogle Scholar
  17. Shewry PR, Halford NG, Lafiandra D (2003) Genetics of wheat gluten proteins. Adv Genet 49:111–184CrossRefGoogle Scholar
  18. Singh N, Shepherd K (1988a) Linkage mapping of genes controlling endosperm storage proteins in wheat. 2. Genes on the long arms of group 1 chromosomes. Theor Appl Genet 75:642–650CrossRefGoogle Scholar
  19. Singh N, Shepherd K (1988b) Linkage mapping of genes controlling endosperm storage proteins in wheat. 1. Genes on the short arms of group 1 chromosomes. Theor Appl Genet 75:628–641CrossRefGoogle Scholar
  20. Singh NK, Shepherd KW, Cornish GB (1991) A simplified SDSPAGE procedure for separating the LMW subunit of glutenin. J Cereal Sci 14:203–208CrossRefGoogle Scholar
  21. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114CrossRefGoogle Scholar
  22. Vitellozzi F, Ciaffi M, Dominici L, Ceoloni C (1997) Isolation of a chromosomally engineered durum wheat line carrying the common wheat Glu-D1d allele. Agronomie 17:413–419Google Scholar
  23. Xu S, Faris JD, Cai X, Klindworth DL (2005) Molecular cytogenetic characterization and seed storage protein analysis of 1A/1D translocation lines of durum wheat. Chromosom Res 13:559–568CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Olivier Coriton
    • 1
    Email author
  • Annie Faye
    • 2
  • Etienne Paux
    • 2
  • Jocelyne Lemoine
    • 1
  • Virginie Huteau
    • 1
  • Gerard Branlard
    • 2
  • Joseph Jahier
    • 1
  1. 1.INRA UMR 1349 IGEPPLe Rheu cedexFrance
  2. 2.INRA UMR 1095 GDECClermont Ferrand cedexFrance

Personalised recommendations