Molecular Breeding

, 39:19 | Cite as

Molecular mapping of the downy mildew and rust resistance genes in a sunflower germplasm line TX16R

  • Z. Liu
  • L. Zhang
  • G. J. Ma
  • G. J. Seiler
  • C. C. Jan
  • L. L. QiEmail author


The sunflower germplasm line TX16R is resistant to Plasmopara halstedii (causal agent of sunflower downy mildew) and Puccinia helianthi (causal agent of sunflower rust), which are two destructive foliar diseases in sunflower production worldwide. This study reports the mapping of the downy mildew and rust resistance genes Pl33 and R16 from TX16R, respectively. Progeny testing of test crosses for downy mildew resistance suggested that Pl33 localizes to linkage group (LG) 4 of the sunflower genome. Molecular mapping of Pl33 using simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers identified Pl33 cosegregating with ORS644, ORS963, SFW04901, and SFW04052, and linking to two SNPs, NSA_006089 and NSA_008496, at a genetic distance of 0.2 cM on the proximal side. Bulked segregant analysis using SSR and EST-SSR markers from LGs previously reported for rust genes identified polymorphic SSR markers associated with rust resistance on LG13. R16 was mapped between SFW08875 and SFW04317 on LG13, with a genetic distance of 1.8 and 1.1 cM, respectively. The 15 linked markers span a genetic distance of 27.4 cM in LG13. The cosegregating or closely linked markers to the two resistance genes will facilitate marker-assisted selection (MAS) and gene pyramiding, and will further assist in identifying genes responsible for DM and rust resistance.


Sunflower Downy mildew Rust Resistance SSR SNP marker 



The authors are very grateful to Lisa Brown for the technical assistance in this study. We thank Ms. Puying Zheng for her help in molecular marker genotyping and Dr. Fang Wei for his help in phenotyping to rust. We appreciate Drs. Yunming Long and Zahirul Talukder for their help during the SNP primer design. This project was supported by the USDA-ARS CRIS Project No. 3060-21000-043-00D. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer.

Author’s contribution

Conceived and designed the experiments: CCJ, ZL. Performed the experiments: ZL, LZ, GJM, CCJ, GS. Analyzed the data: ZL, GJM, LLQ. Wrote the paper: ZL, LLQ. Commented on the manuscript before submission: CCJ, GJM, GS.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The experiments were performed in compliment with the current laws of the USA.

Supplementary material

11032_2018_921_MOESM1_ESM.docx (18 kb)
ESM 1 (DOCX 18 kb)
11032_2018_921_MOESM2_ESM.xlsx (13 kb)
ESM 2 (XLSX 12 kb)
11032_2018_921_MOESM3_ESM.pptx (308 kb)
Supplementary Fig. 1 PCR pattern of seven SNP markers for HA 434 (1), TX16R (2) and HA 458 (3). Four SNP markers had the same PCR pattern between TX16R and HA 458. Three SNP markers, i.e., SFW04052, NSA_006089, and SFW06856, showed different alleles between TX16R (Pl33) and HA 458 (Pl17), with HA 458 having the same allele as HA 434. (PPTX 308 kb)


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Copyright information

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019

Authors and Affiliations

  1. 1.Department of Plant SciencesNorth Dakota State UniversityFargoUSA
  2. 2.Baicheng Academy of Agricultural SciencesBaichengChina
  3. 3.USDA-ARS, Edward T. Schafer Agricultural Research Center, Northern Crop Science LaboratoryFargoUSA

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