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Molecular Breeding

, 39:10 | Cite as

Synchronous improvement of subgenomes in allopolyploid: a case of Sclerotinia resistance improvement in Brassica napus

  • Yijuan Ding
  • Jiaqin Mei
  • Qinan Wu
  • Zhiyong Xiong
  • Yuehua Li
  • Chaoguo Shao
  • Lei Wang
  • Wei Qian
Article
  • 34 Downloads

Abstract

The rare chance of homoelogous exchange results in a low efficiency to transfer elite locus between subgenomes of allopolyploid in vivo. Here, we propose a breeding strategy to synchronously improve the subgenomes of allopolyploid, as a case of Sclerotinia sclerotiorum resistance improvement in Brassica napus. The resistance of both parental species was firstly improved by identifying resistance source in B. oleracea and transferring the resistance loci from resistant B. oleracea into B. rapa. The resistance loci from B. rapa and B. oleracea were then pyramided by resynthesizing B. napus. Four groups of resynthesized B. napus, comprising of 37 lines with or without resistance loci from B. rapa and/or B. oleracea, were evaluated for Sclerotinia resistance across 3 years. Significant differences were found among the four groups for both leaf and stem resistance. The group of resynthesized B. napus carrying resistance loci from B. rapa and B. oleracea exhibited the highest level of resistance, out of which one prominent line showed 2.7-fold higher stem resistance than “Zhongshuang 9,” a partial resistant Chinese rapeseed variety. Our data highlights that the strategy of synchronous improvement of subgenomes can efficiently improve allopolyploids.

Keywords

Allopolyploid Brassica napus Sclerotinia sclerotiorum Subgenome improvement 

Notes

Funding information

This study was financially supported by the 973 Program (2015CB150201), Key Projects in National Science and Technology (2014BAD01B07), National Nature Science Foundation of China (31671726, 31801395), the Science and Technology Innovation Program for the Social Undertakings and the People’s Livelihood in Chongqing (cstc2016shmsx0674, cstc2016shmszx80074, cstc2017shms-xdny80050) and Fundamental Research Funds for the Central Universities (XDJK2018AA004 and XDJK2018B022).

Supplementary material

11032_2018_915_MOESM1_ESM.docx (328 kb)
Supplementary Figure 1 Molecular characterization of the ten female B. rapa individuals. (A) Eight molecular markers flanked with the major QTL on the C09 chromosome of B. oleracea in the previous study (Mei et al. 2013; Ding et al. 2015) were used to screen resistance loci in parent B. rapa individuals. The ‘+’ represents the resistance band and ‘-’ represents no resistance band; red box represents the resistance QTL region; (B) Molecular characterization of parental B. oleracea‘C01’ (lane 1), parental B. rapa ‘6Y733’ (lane 2) and R01-R10 (lane 3–12) revealed with SSR marker ‘SWU01’ (left) and ‘SWU02’ (right). M denotes marker lane; (C) Information of eight markers used to track the resistance loci in the progenies (DOCX 327 kb)
11032_2018_915_MOESM2_ESM.xlsx (13 kb)
Supplementary Table 1 (XLSX 13.1 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of Agronomy and BiotechnologySouthwest UniversityChongqingChina
  2. 2.Inner Mongolia Potato Engineering and Technology Research CentreInner Mongolia UniversityHohhotChina

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