QTL-seq and marker development for resistance to Fusarium oxysporum f. sp. niveum race 1 in cultivated watermelon
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Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. niveum (Fon), is one of the predominant diseases of watermelon. Resistance to Fon race 1 is conferred by a single major quantitative trait locus (QTL), Fo-1.1, but resolution of this region has been poor due to low marker density. In this study, a combination of whole genome resequencing of bulked segregants (QTL-seq analysis) followed by QTL mapping with kompetitive allele specific PCR (KASP) markers developed across Fo-1.1 successfully increased the resolution from 2.03 to 1.56 Mb and 315 kb, respectively. The linkage of the KASP markers to Fon race 1 resistance across a wide range of watermelon germplasm was validated in a set of elite watermelon cultivars. The linked markers described here provide a breeder-friendly toolkit immediately available for high-throughput genotyping in large-scale breeding programs for fine mapping and incorporation of Fon race 1 resistance in watermelon.
KeywordsDisease resistance QTL-seq Fusarium wilt Watermelon Resequencing KASP
This study was funded by the National Institute of Food and Agriculture, project no. 6080-21000-018-08.
Compliance with ethical standards
USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The experiment conducted complies with the laws of the United States.
This research project was developed through a non-funded cooperative agreement (ARS Number: 58-6659-8-102) between USDA-ARS and HM.CLAUSE Seed Company.
This research was supported by a grant from USDA National Institute of Food and Agriculture Specialty Crop Research Initiative (2015-51181-24285).
Conflict of interest
The authors declare that they have no conflict of interest.
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