Expression analysis of vitamin D receptor-associated lncRNAs in epileptic patients

  • Mehrdokht Mazdeh
  • Mehrdad Zamani
  • Mohammad Mahdi Eftekharian
  • Alireza Komaki
  • Shahram Arsang-Jang
  • Mohammad TaheriEmail author
  • Soudeh Ghafouri-FardEmail author
Original Article


Vitamin D has been vastly acknowledged as a neuroactive steroid controlling neurodevelopment. As it exerts its functions through activation of vitamin D receptor (VDR), several studies have assessed the role of VDR in brain function. More recently, a number of long non-coding RNAs (lncRNAs) have been recognized that alter expression of VDR. In the current study, we evaluated expression of four VDR-related lncRNAs (LINC00511, LINC00346, SNHG6 and SNHG16) in peripheral blood of 40 epileptic patients and 39 healthy subjects using quantitative real time PCR method. The relative expression levels of SNHG16 and LINC00511 were higher in epileptic patients compared with healthy subjects. For SNHG16, the difference was only significant between male patients and male controls, while LINC00511 had the opposite pattern. The results of Quantile regression model showed significant associations between SNHG6 and SNHG16 expressions and gender (P values of 0.027 and 0.009 respectively). Significant correlations were detected between expression levels of SNHG6 and SNHG16 (r = 0.32, P = 0.004), SNHG6 and LINC00346 (r = 0.37, P = 0.001), SNHG16 and LINC00346 (r = 0.30, P = 0.007) as well as SNHG16 and LINC00511 (r = 0.29, P = 0.009). Expression of LINC00346 was inversely correlated with vitamin D levels only in male epileptic patients (r = −0.58, P = 0.011). Expression of SNHG6 was correlated with vitamin D levels in male controls but no other subgroups (r = 0.51, P = 0.044). Based on the results of ROC curve analysis, SNHG16 had the diagnostic power of 0.86 in male subjects. Taken together, the current study provides evidences for dys-regulation of VDR-related lncRNAs in epileptic patients. The clinical significance of these finding should be explored in future studies.


Epilepsy VDR LINC00511 LINC00346 SNHG6 SNHG16 lncRNA 



The current study was supported by a grant from Hamadan University of Medical Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare they have no conflict of interest.

Supplementary material

11011_2019_446_MOESM1_ESM.pdf (191 kb)
ESM 1 (PDF 190 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Neurophysiology Research CenterHamadan University of Medical SciencesHamadanIran
  2. 2.Department of NeurologyHamadan University of Medical SciencesHamadanIran
  3. 3.Clinical Research Development Center (CRDU)Qom University of Medical SciencesQomIran
  4. 4.Urogenital Stem Cell Research CenterShahid Beheshti University of Medical SciencesTehranIran
  5. 5.Department of Medical GeneticsShahid Beheshti University of Medical SciencesTehranIran

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