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Metabolic Brain Disease

, Volume 34, Issue 1, pp 61–69 | Cite as

Role of PUMA in the methamphetamine-induced migration of microglia

  • Lei Zhao
  • Longfei Du
  • Yanhong Zhang
  • Jie Chao
  • Ming Duan
  • Honghong Yao
  • Chuanlu Shen
  • Yuan ZhangEmail author
Original Article
  • 112 Downloads

Abstract

In this study, we demonstrated that PUMA was involved in the microglial migration induced by methamphetamine. PUMA expression was examined by western blotting and immunofluorescence staining. BV2 and HAPI cells were pretreated with a sigma-1R antagonist and extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase (MAPK), c-Jun N-terminal protein kinase (JNK), and phosphatidylinositol-3 kinase (PI3K)/Akt inhibitors, and PUMA expression was detected by western blotting. The cell migration in BV2 and HAPI cells transfected with a lentivirus encoding red fluorescent protein (LV-RFP) was also examined using a wound-healing assay and nested matrix model and cell migration assay respectively. The molecular mechanisms of PUMA in microglial migration were validated using a siRNA approach. The exposure of BV2 and HAPI cells to methamphetamine increased the expression of PUMA, reactive oxygen species (ROS), the MAPK and PI3K/Akt pathways and the downstream transcription factor signal transducer and activator of transcription 3 (STAT3) pathways. PUMA knockdown in microglia transfected with PUMA siRNA attenuated the increased cell migration induced by methamphetamine, thereby implicating PUMA in the migration of BV2 and HAPI cells. This study demonstrated that methamphetamine-induced microglial migration involved PUMA up-regulation. Targeting PUMA could provide insights into the development of a potential therapeutic approach for the alleviation of microglia migration induced by methamphetamine.

Keywords

PUMA Methamphetamine Microglia Migration 

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (No. 81322048 and No. 81473190).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Lei Zhao
    • 1
  • Longfei Du
    • 1
  • Yanhong Zhang
    • 1
  • Jie Chao
    • 2
  • Ming Duan
    • 3
  • Honghong Yao
    • 1
    • 4
  • Chuanlu Shen
    • 5
  • Yuan Zhang
    • 1
    Email author
  1. 1.Department of PharmacologyMedical School of Southeast University, Southeast UniversityNanjingChina
  2. 2.Department of PhysiologyMedical School of Southeast University, Southeast UniversityNanjingChina
  3. 3.Key Laboratory for Zoonosis Research, Ministry of EducationJilin UniversityChangchunChina
  4. 4.Institute of Life Sciences, Key Laboratory of Developmental Genes and Human DiseaseSoutheast UniversityNanjingChina
  5. 5.Department of PathophysiologyMedical School of Southeast University, Southeast UniversityNanjingChina

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