Gradual common carotid artery occlusion as a novel model for cerebrovascular Hypoperfusion
Chronic cerebrovascular hypoperfusion results in vascular dementia and increases predisposition to lacunar infarcts. However, there are no suitable animal models. In this study, we developed a novel model for chronic irreversible cerebral hypoperfusion in mice. Briefly, an ameroid constrictor was placed on the right carotid artery to gradually occlude the vessel, while a microcoil was placed on the left carotid artery to prevent compensation of the blood flow. This procedure resulted in a gradual hypoperfusion developing over a period of 34 days with no cerebral blood flow recovery. Histological analysis of the brain revealed neuronal and axonal degeneration as well as necrotic lesions. The most severely affected regions were located in the hippocampus and the corpus callosum. Overall, our paradigm is a viable model to study brain pathology resulting from gradual cerebrovascular hypoperfusion.
KeywordsWhite matter pathology Hypoperfusion Small vessel disease Gradual vessel occlusion Cerebral blood flow Neuronal degeneration
The authors thank Dr. Gregory Konat for revising and editing the manuscript. This study was supported by the Helen Marie Lewis Medical Research Foundation (to JWS, XR, and AM), NIH CoBRE (P20 GM109098 to JWS), AHA SDG (16SDG31170008 to XR), NIH T32 (AG052375 to JWS), and funding from the Department of Neurology at WVU (to AM).
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