Baicalin augments the differentiation of osteoblasts via enhancement of microRNA-217
Baicalin (BAI), a sort of flavonoid monomer, acquires from Scutellaria baicalensis Georgi, which was forcefully reported in diversified ailments due to the pleiotropic properties. But, the functions of BAI in osteoblast differentiation have not been addressed. The intentions of this study are to attest the influences of BAI in the differentiation of osteoblasts. MC3T3-E1 cells or rat primary osteoblasts were exposed to BAI, and then cell viability, ALP activity, mineralization process, and Runx2 and Ocn expression were appraised through implementing CCK-8, p-nitrophenyl phosphate (pNPP), Alizarin red staining, western blot, and RT-qPCR assays. The microRNA-217 (miR-217) expression was evaluated in MC3T3-E1 cells or rat primary osteoblasts after BAI disposition; meanwhile, the functions of miR-217 in BAI-administrated MC3T3-E1 cells were estimated after miR-217 inhibitor transfection. The impacts of BAI and miR-217 inhibition on Wnt/β-catenin and MEK/ERK pathways were probed to verify the involvements in BAI-regulated the differentiation of osteoblasts. BAI accelerated cell viability, osteoblast activity, and Runx2 and Ocn expression in MC3T3-E1 cells or rat primary osteoblasts, and the phenomena were mediated via activations of Wnt/β-catenin and MEK/ERK pathways. Elevation of miR-217 was observed in BAI-disposed MC3T3-E1 cells or rat primary osteoblasts, and miR-217 repression annulled the functions of BAI in MC3T3-E1 cell viability and differentiation. Additionally, the activations of Wnt/β-catenin and MEK/ERK pathways evoked by BAI were both restrained by repression of miR-217. These explorations uncovered that BAI augmented the differentiation of osteoblasts via activations of Wnt/β-catenin and MEK/ERK pathways by ascending miR-217 expression.
KeywordsBone fracture Baicalin microRNA-217 Wnt/β-catenin MEK/ERK
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Conflict of interest
The authors declare that they have no conflict of interest.
- 9.Lai W, Jia J, Yan B, Jiang Y, Shi Y, Chen L, Mao C, Liu X, Tang H, Gao M, Cao Y, Liu S, Tao Y (2017) Baicalin hydrate inhibits cancer progression in nasopharyngeal carcinoma by affecting genome instability and splicing. Oncotarget 9(1):901–914. https://doi.org/10.18632/oncotarget.22868 CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Lin D, Du Q, Wang H, Gao G, Zhou J, Ke L, Chen T, Shaw C, Rao P (2017) Antidiabetic micro-/nanoaggregates from ge-gen-qin-lian-tang decoction increase absorption of baicalin and cellular antioxidant activity in vitro. Biomed Res Int 2017:9217912. https://doi.org/10.1155/2017/9217912 CrossRefPubMedPubMedCentralGoogle Scholar
- 16.Wu X, Zhi F, Lun W, Deng Q, Zhang W (2018) Baicalin inhibits PDGF-BB-induced hepatic stellate cell proliferation, apoptosis, invasion, migration and activation via the miR-3595/ACSL4 axis. Int J Mol Med 41(4):1992–2002. https://doi.org/10.3892/ijmm.2018.3427 CrossRefPubMedPubMedCentralGoogle Scholar
- 23.Guo AJ, Choi RC, Cheung AW, Chen VP, Xu SL, Dong TT, Chen JJ, Tsim KW (2011) Baicalin, a flavone, induces the differentiation of cultured osteoblasts: an action via the Wnt/beta-catenin signaling pathway. J Biol Chem 286(32):27882–27893. https://doi.org/10.1074/jbc.M111.236281 CrossRefPubMedPubMedCentralGoogle Scholar
- 24.Noguchi T, Ebina K, Hirao M, Otsuru S, Guess AJ, Kawase R, Ohama T, Yamashita S, Etani Y, Okamura G, Yoshikawa H (2018) Apolipoprotein E plays crucial roles in maintaining bone mass by promoting osteoblast differentiation via ERK1/2 pathway and by suppressing osteoclast differentiation via c-Fos, NFATc1, and NF-kappaB pathway. Biochem Biophys Res Commun 503(2):644–650. https://doi.org/10.1016/j.bbrc.2018.06.055 CrossRefPubMedGoogle Scholar
- 26.Bustos F, Sepúlveda H, Prieto CP, Carrasco M, Díaz L, Palma J, Lattus J, Montecino M, Palma V (2017) RUNX2 induction during differentiation of Wharton’s jelly mesenchymal stem cells to osteoblasts is regulated by JARID1B histone demethylase. Stem Cells (Dayton, Ohio) 35(12):2430–2441CrossRefGoogle Scholar
- 31.Huang Z, Cheng C, Wang J, Liu X, Wei H, Han Y, Yang S, Wang X (2018) Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3-E1 cells through microRNA-153 by targeting Runt-related transcription factor 2. Exp Ther Med 15(6):5159–5166. https://doi.org/10.3892/etm.2018.6127 CrossRefPubMedPubMedCentralGoogle Scholar
- 34.Fei Y, Xiao L, Doetschman T, Coffin DJ, Hurley MM (2011) Fibroblast growth factor 2 stimulation of osteoblast differentiation and bone formation is mediated by modulation of the Wnt signaling pathway. J Biol Chem 286(47):40575–40583. https://doi.org/10.1074/jbc.M111.274910 CrossRefPubMedPubMedCentralGoogle Scholar
- 36.Guo C, Yang RJ, Jang K, Zhou XL, Liu YZ (2017) Protective effects of pretreatment with quercetin against lipopolysaccharide-induced apoptosis and the inhibition of osteoblast differentiation via the MAPK and Wnt/beta-catenin pathways in MC3T3-E1 Cells. Cell Physiol Biochem 43(4):1547–1561. https://doi.org/10.1159/000481978 CrossRefPubMedGoogle Scholar