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Molecular and Cellular Biochemistry

, Volume 462, Issue 1–2, pp 167–172 | Cite as

MicroRNA signature of human blood mononuclear cells

  • Qiuwang Zhang
  • Anthony Cannavicci
  • Si-Cheng Dai
  • Chenxi Wang
  • Michael J. B. KutrykEmail author
Article
  • 63 Downloads

Abstract

MicroRNAs (miRNAs) regulate a wide range of cellular processes and functions. Blood mononuclear cells (BMNCs) participate in the immune response, inflammatory reaction and angiogenesis. In 2010, a total of 157 miRNAs were quantified by RT-qPCR and a miRNA signature was determined for human peripheral BMNCs. With the advent of technologies such as RNA sequencing, many new miRNAs have been identified. This study was designed to provide an up-to-date miRNA signature for human BMNCs. Peripheral BMNCs were isolated by Ficoll density gradient centrifugation. Using the qPCR array assay, we identified 108 highly expressed miRNAs (Ct value < 30) in human BMNCs. Further validation of the array results by quantifying select miRNAs with RT-qPCR revealed a strong correlation between Ct values derived from array analysis and RT-qPCR, suggesting the array results presented in this study are accurate and reliable. Of note, the function of the majority of the highly expressed miRNAs we have identified has not yet been studied. Our findings may help direct further studies of the regulatory roles of miRNAs in BMNC function.

Keywords

MicroRNA Blood mononuclear cell Microarray analysis RT-qPCR 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Cardiology, Keenan Research Center for Biomedical Science, St. Michael’s HospitalUniversity of TorontoTorontoCanada
  2. 2.Institute of Medical ScienceUniversity of TorontoTorontoCanada
  3. 3.Department of Cardiovascular Surgery, Renji HospitalShanghai Jiaotong UniversityShanghaiChina
  4. 4.St. Michael’s HospitalTorontoCanada

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