Molecular and Cellular Biochemistry

, Volume 462, Issue 1–2, pp 107–114 | Cite as

Vaspin protects mouse mesenchymal stem cells from oxidative stress-induced apoptosis through the MAPK/p38 pathway

  • Xiao Zhu
  • Lingyan Zhang
  • Youming Chen
  • Bo Chen
  • Haifeng Huang
  • Jicheng Lv
  • Shidi Hu
  • Jie ShenEmail author


The aim of the work was to study the influence of vaspin on oxidative stress-induced apoptosis of mouse mesenchymal stem cells (MSCs). MSCs originated from bone marrow of C57BL/6 mouse were treated with vaspin and/or H2O2 in a dose-dependent manner. Cellular viability detected by CCK-8 and cell apoptosis studied by flow cytometry and TUNEL assay were observed in these cells. The protein expressions of PI3K, p-PI3K, Akt, p-Akt, T-ERK1/2, p-ERK1/2, p38, p-p38, JNK, and p-JNK were tested by Western blot. Vaspin had no significant effect on cellular viability, but significantly reduced H2O2-induced apoptosis. Western blot assay showed that pretreatment with vaspin promoted the activation of p-p38. Inhibition of p38 by SB203580 suppressed the protective effect of vaspin on oxidative stress-induced apoptosis. Vaspin inhibits oxidative stress-induced apoptosis of MSCs via the activation of MAPK/p38 signaling pathway. These findings indicate that vaspin is prone to osteoporosis protection.


Vaspin Mesenchymal stem cells Apoptosis 



This study was funded by the National Natural Science Foundation of China (Grant No. 81400849); the Natural Science Foundation of Guangdong Province (Grant No. 2014A030310490); the Science and Technology Planning Project of Guangdong Province, China (Grant No. 2017A020215189); the Science and Technology Planning Project of Tianhe District, Guangdong Province, China (Grant No. 2013kw004).

Compliance with ethical standards

Conflict of interest

The authors declare that they there are no competing interests.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Endocrinology and MetabolismThe Third Affiliated Hospital of Southern Medical UniversityGuangzhouChina
  2. 2.Department of Medical ImageThe Third Affiliated Hospital of Southern Medical UniversityGuangzhouChina
  3. 3.Department of Medical LaboratoryThe Third Affiliated Hospital of Southern Medical UniversityGuangzhouChina
  4. 4.Department of EndocrinologyGuangdong Second Provincial HospitalGuangzhouChina
  5. 5.Department of Internal MedicineThe First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouChina

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